Multi-lot analysis of custom collagenase enzyme blend in human islet isolations

Transplantation Proceedings

Volumn 37, Issue 8, 2005, Pages 3417-3419

  O'Gorman, D ^a   Kin, T ^a   McGhee Wilson, D ^a   Shapiro, A M J ^a   Lakey, J R T ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CADAVER; CELL ISOLATION; CHI SQUARE TEST; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; MEDICAL RECORD; ORGAN WEIGHT; PANCREAS; PANCREAS ISLET CELL; PRIORITY JOURNAL; RETROSPECTIVE STUDY; STANDARDIZATION; STATISTICAL SIGNIFICANCE; STUDENT T TEST; TREATMENT OUTCOME;

BRAIN DEATH; CELL SEPARATION; COLLAGENASES; HUMANS; ISLETS OF LANGERHANS; RETROSPECTIVE STUDIES; THERMOLYSIN; TISSUE AND ORGAN HARVESTING;

EID: 27844524977     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2005.09.139     Document Type: Conference Paper

References (8)

1. A.M.J. Shapiro, J.R.T. Lakey, E.A. Ryan Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen N Engl J Med 343 2000 230
2. E.A. Ryan, J.R.T. Lakey, R.V. Rajotte Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol Diabetes 50 2001 710
3. L.H. Toledo-Pereyra, M. Zammit, S. Malcom Inconsistency of collagenase activity for the isolation of islet cells for transplantation Transplantation 27 1979 222
4. H. Brandhorst, D. Brandhorst, B.J. Hering Significant progress in porcine islet mass isolation utilizing Liberase HI for enzymatic low temperature pancreas digestion Transplantation 68 1999 355
5. P. Bucher, D. Bosco, D. Mathe Optimization of neutral protease to collagenase activity ratio for islets of Langerhans isolation Transplant Proc 36 2004 1145
6. H. Brandhorst, A. Alt, N. Huettler The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas Transplant Proc 37 2005 215
7. H. Brandhorst, M.D. Brendel, M. Eckhard Influence of neutral protease activity on human islet isolation outcome Transplant Proc 37 2005 241
8. D. Brandhorst, M. Iken, M.D. Brendel Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas Transplant Proc 37 2005 458