Infection, excretion and seroconversion dynamics of porcine circovirus type 2 (PCV2) in pigs from post-weaning multisystemic wasting syndrome (PMWS) affected farms in Spain and Denmark

Veterinary Microbiology

Volumn 135, Issue 3-4, 2009, Pages 272-282

  Grau Roma, Llorenç ^a,b   Hjulsager, Charlotte K ^c   Sibila, Marina ^a   Kristensen, Charlotte S ^d   López Soria, Sergio ^a   Enøe, Claes ^c   Casal, Jordi ^a,b   Bøtner, Anette ^c   Nofrarías, Miquel ^a   Bille Hansen, Vivi ^c   Fraile, Lorenzo ^a,e   Baekbo, Poul ^d   Segalés, Joaquim ^a,b   Larsen, Lars E ^c  

^a CENTRE DE RECERCA EN SANITAT ANIMAL CRESA   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^d Fed Danish Pig Producers S   (Denmark)

^e IRTA   (Spain)

Author keywords

Epidemiology; Longitudinal case control study; Porcine circovirus type 2 (PCV2); Post weaning multisystemic wasting syndrome (PMWS); Quantitative real time PCR (qPCR)

Indexed keywords

MATERNAL ANTIBODY;

ANIMAL TISSUE; ANTIBODY TITER; ARTICLE; CASE CONTROL STUDY; CIRCOVIRIDAE; CONTROLLED STUDY; DENMARK; HISTOPATHOLOGY; LONGITUDINAL STUDY; LYMPHOID TISSUE; NONHUMAN; NOSE SMEAR; PIG FARMING; POLYMERASE CHAIN REACTION; POSTWEANING MULTISYSTEMIC WASTING SYNDROME; SENSITIVITY AND SPECIFICITY; SEROCONVERSION; SERODIAGNOSIS; SPAIN; VIRUS DETECTION; VIRUS EXCRETION; VIRUS INFECTION; VIRUS LOAD;

AGING; ANIMALS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DENMARK; DISEASE OUTBREAKS; NOSE; POLYMERASE CHAIN REACTION; RECTUM; SPAIN; SWINE; SWINE DISEASES; VIRAL LOAD; WASTING SYNDROME; WEANING;

PORCINE CIRCOVIRUS 2; SUIDAE;

EID: 61849151905     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2008.10.007     Document Type: Article

References (39)

1. Allan G.M., Kennedy S., McNeilly F., Foster J.C., Ellis J.A., Krakowka S.J., Meehan B.M., and Adair B.M. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J. Comp. Pathol. 121 (1999) 1-11
2. Armstrong, D., Bishop, S., 2004. Does genetics or litter effect influence mortality in PMWS. Proceedings of the International Pig Veterinary Society Congress. p. 809.
3. Brunborg I.M., Moldal T., and Jonassen C.M. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. J. Virol. Methods 122 (2004) 171-178
4. Calsamiglia M., Fraile L., Espinal A., Cuxart A., Seminati C., Martin M., Mateu E., Domingo M., and Segales J. Sow porcine circovirus type 2 (PCV2) status effect on litter mortality in postweaning multisystemic wasting syndrome (PMWS). Res. Vet. Sci. 82 (2007) 299-304
5. Caprioli A., McNeilly F., McNair I., Lagan-Tregaskis P., Ellis J., Krakowka S., McKillen J., Ostanello F., and Allan G. PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs. Res. Vet. Sci. 81 (2006) 287-292
6. Carasova P., Celer V., Takacova K., Trundova M., Molinkova D., Lobova D., and Smola J. The levels of PCV2 specific antibodies and viremia in pigs. Res. Vet. Sci. 83 (2007) 274-278
7. Cler L., Bu D., Lewis C., and Euhus D. A comparison of five methods for extracting DNA from paucicellular clinical samples. Mol. Cell. Probes 20 (2006) 191-196
8. EnØe, C., Baekbo, P., Vigre, H., Larsen, L.E., Jorsal, S.E., Nielsen, E.O., 2006. Serological testing for porcine circovirus type 2 in Danish pig herds with and without PMWS. Proceedings of the International Symposium on Emerging and Re-emerging Pig Diseases. p. 102.
9. Fahle G.A., and Fischer S.H. Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens. J. Clin. Microbiol. 38 (2000) 3860-3863
10. Fort M., Olvera A., Sibila M., Segales J., and Mateu E. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet. Microbiol. 125 (2007) 244-255
11. Grasland B., Loizel C., Blanchard P., Oger A., Nignol A.C., Bigarre L., Morvan H., Cariolet R., and Jestin A. Reproduction of PMWS in immunostimulated SPF piglets transfected with infectious cloned genomic DNA of type 2 porcine circovirus. Vet. Res. 36 (2005) 685-697
12. Grau-Roma L., Crisci E., Sibila M., Lopez-Soria S., Nofrarias M., Cortey M., Fraile L., Olvera A., and Segales J. A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet. Microbiol. 128 (2008) 23-35
13. Harding J.C.E. Recognizing and diagnosing porcine multisystemic wasting syndrome (PMWS). J. Swine Health Prod. 5 (1997) 201-203
14. Hassing, A.G., Bøtner, A., Ladekjær-Mikkelsen, A.-S., Bækbo, P., Jorsal, S.E., Bille-Hansen, V., 2002. Postweaning multisystemic wasting syndrome in Denmark. Proceedings of the Congress of the International Pig Veterinary Society. p. 173.
15. Hjulsager C., Grau-Roma L., Sibila M., EnØe C., Larsen L.E., and Segalés J. Inter-laboratory comparison on two real-time PCR techniques for quantification of PCV2 nucleic acid extracted from field samples. Vet. Microbiol. 133 (2009) 172-178
16. Jensen T.K., Vigre H., Svensmark B., and Bille-Hansen V. Distinction between porcine circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis. J. Comp. Pathol. 135 (2006) 176-182
17. Krakowka S., Ellis J., McNeilly F., Waldner C., and Allan G. Features of porcine circovirus-2 disease: correlations between lesions, amount and distribution of virus, and clinical outcome. J. Vet. Diagn. Invest. 17 (2005) 213-222
18. Krakowka S., Ellis J., McNeilly F., Waldner C., Rings D.M., and Allan G. Mycoplasma hyopneumoniae bacterins and porcine circovirus type 2 (PCV2) infection: induction of postweaning multisystemic wasting syndrome (PMWS) in the gnotobiotic swine model of PCV2-associated disease. Can. Vet. J. 48 (2007) 716-724
19. Larochelle R., Magar R., and D'Allaire S. Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. Can. J. Vet. Res. 67 (2003) 114-120
20. López-Soria S., Segales J., Rose N., Vinas M.J., Blanchard P., Madec F., Jestin A., Casal J., and Domingo M. An exploratory study on risk factors for postweaning multisystemic wasting syndrome (PMWS) in Spain. Prev. Vet. Med. 69 (2005) 97-107
21. McIntosh K.A., Harding J.C., Ellis J.A., and Appleyard G.D. Detection of Porcine circovirus type 2 viremia and seroconversion in naturally infected pigs in a farrow-to-finish barn. Can. J. Vet. Res. 70 (2006) 58-61
22. Meerts P., Misinzo G., Lefebvre D., Nielsen J., Botner A., Kristensen C.S., and Nauwynck H.J. Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet. Res. 2 (2006) 6
23. Olvera A., Sibila M., Calsamiglia M., Segales J., and Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J. Virol. Methods 117 (2004) 75-80
24. Opriessnig T., McKeown N.E., Zhou E.M., Meng X.J., and Halbur P.G. Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence. J. Gen. Virol. 87 (2006) 2923-2932
25. Pensaert M.B., Sanchez Jr. R.E., Ladekjaer-Mikkelsen A.S., Allan G.M., and Nauwynck H.J. Viremia and effect of fetal infection with porcine viruses with special reference to porcine circovirus 2 infection. Vet. Microbiol. 98 (2004) 175-183
26. Quintana J., Segalés J., Rosell C., Calsamiglia M., Rodriguez-Arrioja G.M., Chianini F., Folch J.M., Maldonado J., Canal M., Plana-Duran J., and Domingo M. Clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome. Vet. Rec. 149 (2001) 357-361
27. Rodríguez-Arrioja G.M., Segalés J., Calsamiglia M., Resendes A.R., Balasch M., Plana-Duran J., Casal J., and Domingo M. Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome. Am. J. Vet. Res. 63 (2002) 354-357
28. Rodríguez-Arrioja G.M., Segalés J., Balasch M., Rosell C., Quintant J., Folch J.M., Plana-Duran J., Mankertz A., and Domingo M. Serum antibodies to porcine circovirus type 1 and type 2 in pigs with and without PMWS. Vet. Rec. 146 (2000) 762-764
29. Rose N., Larour G., Le Diguerher G., Eveno E., Jolly J.P., Blanchard P., Oger A., Le Dimna M., Jestin A., and Madec F. Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 French farrow-to-finish herds. Prev. Vet. Med. 61 (2003) 209-225
30. Rosell C., Segalés J., Plana-Duran J., Balasch M., Rodríguez-Arrioja G.M., Kennedy S., Allan G.M., McNeilly F., Latimer K.S., and Domingo M. Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J. Comp. Pathol. 120 (1999) 59-78
31. Segalé s, J., Larsen, L., Wallgren, P., Rose, N., Grau-Roma, L., Sibila, M., Fraile, L., Casal, J., Baekbo, P., 2007. What do we know on epidemiology, control and prevention of porcine circovirus diseases? Proceedings of the International Symposium on Emerging and Re-emerging Pig Diseases. p. 35.
32. Segalés J., Allan G.M., and Domingo M. Porcine circovirus diseases. Anim. Health Res. Rev. 6 (2005) 119-142
33. Segalés J., Calsamiglia M., Olvera A., Sibila M., Badiella L., and Domingo M. Quantification of porcine circovirus type 2 (PCV2) DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without postweaning multisystemic wasting syndrome (PMWS). Vet. Microbiol. 111 (2005) 223-229
34. Segalés J., Domingo M., Chianini F., Majo N., Dominguez J., Darwich L., and Mateu E. Immunosuppression in postweaning multisystemic wasting syndrome affected pigs. Vet. Microbiol. 98 (2004) 151-158
35. Segalés J. Update on postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome diagnostics. J. Swine Health Prod. 10 (2002) 277-281
36. Segalés J., Sitjar M., Domingo M., Dee S., Del Pozo M., Noval R., Sacristan C., De las Heras A., Ferro A., and Latimer K.S. First report of post-weaning multisystemic wasting syndrome in pigs in Spain. Vet. Rec. 141 (1997) 600-601
37. Shibata I., Okuda Y., Kitajima K., and Asai T. Shedding of porcine circovirus into colostrum of sows. J. Vet. Med. B Infect. Dis. Vet. Public Health 53 (2006) 278-280
38. Sibila M., Calsamiglia M., Segales J., Blanchard P., Badiella L., Le Dimna M., Jestin A., and Domingo M. Use of a polymerase chain reaction assay and an ELISA to monitor porcine circovirus type 2 infection in pigs from farms with and without postweaning multisystemic wasting syndrome. Am. J. Vet. Res. 65 (2004) 88-92
39. Vigre H., Baekbo P., Jorsal S.E., Bille-Hansen V., Hassing A.G., Enoe C., and Botner A. Spatial and temporal patterns of pig herds diagnosed with Postweaning Multisystemic Wasting Syndrome (PMWS) during the first two years of its occurrence in Denmark. Vet. Microbiol. 110 (2005) 17-26