PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs

Research in Veterinary Science

Volumn 81, Issue 2, 2006, Pages 287-292

  Caprioli, A ^a   McNeilly, F ^b   McNair, I ^b   Lagan Tregaskis, P ^b   Ellis, J ^c   Krakowka, S ^d   McKillen, J ^b   Ostanello, F ^a   Allan, G ^b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b AGRI FOOD AND BIOSCIENCES INSTITUTE   (United Kingdom)

^c UNIVERSITY OF SASKATCHEWAN   (Canada)

^d OHIO STATE UNIVERSITY   (United States)

Author keywords

Diagnosis; PCR; PCV2; Pig; PMWS; Porcine circovirus type 2; Post weaning multisystemic wasting syndrome

Indexed keywords

VIRUS ANTIGEN;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD ANALYSIS; CIRCOVIRIDAE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA DETERMINATION; DNA VIRUS; EARLY DIAGNOSIS; EXPERIMENTAL ANIMAL; FECES CULTURE; GENE AMPLIFICATION; HISTOPATHOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; SAMPLE SIZE; STATISTICAL SIGNIFICANCE; SWINE DISEASE; TONSIL; VIROLOGY; VIRUS INFECTION; WASTING SYNDROME; WEANING;

ANIMALS; ANTIGENS, VIRAL; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DNA, VIRAL; FECES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HISTOCYTOCHEMISTRY; POLYMERASE CHAIN REACTION; RANDOM ALLOCATION; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; SWINE DISEASES; TONSIL; WASTING SYNDROME;

ANIMALIA; PORCINE CIRCOVIRUS 2;

EID: 33745202642     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2006.01.001     Document Type: Article

References (22)

1. Allan G.M., and Ellis J.A. Porcine circoviruses: a review. Journal of Veterinary Diagnostic Investigation 12 (2000) 3-14
2. Allan G.M., Kennedy S., McNeilly F., Foster J.C., Ellis J.A., Krakowka S.J., Meehan B.M., and Adair B.M. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. Journal of Comparative Pathology 121 (1999) 1-11
3. Allan G.M., Mc Neilly F., Meehan B.M., Kennedy S., Mackie D.P., Ellis J.A., Clark E.G., Espuna E., Saubi N., Riera P., Bøtner A., and Charreyre C.E. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Veterinary Microbiology 66 (1999) 115-123
4. Brunborg I.M., Moldal T., and Jonassen C.M. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. Journal of Virological Methods 122 (2004) 171-178
5. Calsamiglia M., Segales J., Quintana J., Rosell C., and Domingo M. Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. Journal of Clinical Microbiology 40 (2002) 1848-1850
6. Chae C. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Veterinary Journal 168 (2004) 41-49
7. Darwich L., Segales J., and Mateu E. Pathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2: An immune riddle. Archives of Virology 149 (2004) 857-874
8. Ellis J., Hassard L., Clark E., Harding J., Allan G., Willson P., Strokappe J., Martin K., McNeilly F., Meehan B., Todd D., and Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Canadian Veterinary Journal 39 (1998) 44-51
9. Krakowka S., Ellis J., Meehan B., Kennedy S., Mc Neilly F., and Allan G.M. Viral wasting syndrome of swine: experimental reproduction of PMWS in gnotobiotic swine by coinfection with porcine circovirus-2 (PCV-2) and porcine parvovirus (PPV). Veterinary Pathology 37 (2000) 254-263
10. Krakowka S., Ellis J., McNeilly F., Waldner C., and Allan G. Features of porcine circovirus-2 disease: correlations between lesions, amount and distribution of virus, and clinical outcome. Journal of Veterinary Diagnostic Investigation 17 (2005) 213-222
11. Ladekjaer-Mikkelsen A.S., Nielsen J., Stadejek T., Storgaard T., Krakowka S., Ellis J., McNeilly F., Allan G., and Botner A. Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Veterinary Microbiology 89 (2002) 97-114
12. Larochelle R., Bielanski A., Müller P., and Magar R. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. Journal of Clinical Microbiology 384 (2000) 629-632
13. Liu Q., Wang L., Willson P., and Babiuk L.A. Quantitative, competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome. Journal of Clinical Microbiology 38 (2000) 3474-3477
14. Magar R., Larochelle R., Thibault S., and Lamontagne L. Experimental trasmission of porcine circovirus type 2 (PCV2) in weaned pigs: a sequential study. Journal of Comparative Pathology 123 (2000) 258-269
15. McNair I., Marshall M., McNeilly F., Botner A., Ladekjaer-Mikkelsen A.S., Vincent I., Herrmann B., Sanchez R., and Rhodes C. Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2. Journal of Veterinary Diagnostic Investigation 16 (2004) 164-166
16. McNeilly F., McNair I., Mackie D.P., Meehan B.M., Kennedy S., Moffett D., Ellis J.A., Krakowka S., and Allan G.M. Production, characterization and applications of monoclonal antibodies to PCV2. Archives of Virology 146 (2001) 1-14
17. Meehan B., McNeilly F., Todd D., Kennedy K., Jewhurst V., Ellis J., Hassard L., Clark C., Haines D., and Allan G. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. Journal of General Virology 79 (1998) 2171-2179
18. Olvera A., Sibila M., Calsamiglia M., Segales J., and Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. Journal of Virological Methods 117 (2004) 75-80
19. Ouardani M., Wilson L., Jette R., Montpetit C., and Dea S. Multiplex PCR for detection and typing of porcine circoviruses. Journal of Clinical Microbiology 37 (1999) 3917-3924
20. Segalés J., and Domingo M. Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. Veterinary Quarterly 24 (2002) 109-124
21. Shibata I., Okuda Y., Yazawa S., Ono M., Sasaki T., Itagaki M., Nakajima N., Okabe Y., and Hidejima I. PCR detection of porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases. Journal of Veterinary Medical Science 65 (2003) 405-408
22. Tischer I., Gelderblom H., Vettermann W., and Koch M.A. A very small porcine virus with circular single-stranded DNA. Nature 295 (1982) 64-66