Purification and characterization of recombinant human soluble guanylate cyclase produced from baculovirus-infected insect cells

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 133-139

  Emmons, Thomas L ^a   Mathis, Karl J ^a   Shuck, Mary E ^a   Reitz, Beverly A ^a   Curran, Daniel F ^a   Walker, Mark C ^a   Leone, Joseph W ^a   Day, Jacqueline E ^a   Bienkowski, Michael J ^a   Fischer, H David ^a   Tomasselli, Alfredo G ^a  

^a PFIZER INC   (United States)

Author keywords

3 ethyl 3 (ethylaminoethyl) 1 hydroxy 2 oxo 1 triazene (NOC 12); GTP agarose; Protein purification; Soluble guanylate cyclase (sGC)

Indexed keywords

CELL RECEPTOR; GUANYLATE CYCLASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); RECOMBINANT PROTEIN; SEPHAROSE; SOLUBLE GUANYLYL CYCLASE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BACULOVIRUS; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; ENZYME ACTIVATION; GENETICS; HUMAN; INSECT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; VIROLOGY;

ANIMALS; BACULOVIRIDAE; CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; GUANYLATE CYCLASE; HUMANS; INSECTS; KINETICS; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT PROTEINS; SEPHAROSE;

HEXAPODA;

EID: 61749099316     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.01.001     Document Type: Article

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