Preparation of isotopically labelled recombinant β-defensin for NMR studies

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 179-184

  Seo, Emily S ^a   Vargues, Thomas ^a   Clarke, David J ^a   Uhrín, Dušan ^a   Campopiano, Dominic J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

15N; Antimicrobial peptide; Defensin; Isotopic label; Mass spectrometry; NMR

Indexed keywords

BETA DEFENSIN; DEFB4 PROTEIN, HUMAN; HYBRID PROTEIN; NITROGEN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; STANDARD; X RAY CRYSTALLOGRAPHY;

BETA-DEFENSINS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; NITROGEN ISOTOPES; RECOMBINANT FUSION PROTEINS; REFERENCE STANDARDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 61749093995     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.11.007     Document Type: Article

References (42)

1. Ganz T., Selsted M.E., Szklarek D., Harwig S.S., Daher K., Bainton D.F., and Lehrer R.I. Defensins. Natural peptide antibiotics of human neutrophils. J. Clin. Invest. 76 (1985) 1427-1435
2. Kluver E., Adermann K., and Schulz A. Synthesis and structure-activity relationship of beta-defensins, multi-functional peptides of the immune system. J. Pept. Sci. 12 (2006) 243-257
3. Oppenheim J.J., Biragyn A., Kwak L.W., and Yang D. Roles of antimicrobial peptides such as defensins in innate and adaptive immunity. Ann. Rheum. Dis. 62 (2003) 17-21
4. Pazgier M., Hoover D.M., Yang D., Lu W., and Lubkowski J. Human beta-defensins. Cell. Mol. Life Sci. 63 (2006) 1294-1313
5. Tang Y.Q., Yuan J., Osapay G., Osapay K., Tran D., Miller C.J., Ouellette A.J., and Selsted M.E. A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated alpha-defensins. Science 286 (1999) 498-502
6. Cole A.M., Hong T., Boo L.M., Nguyen T., Zhao C., Bristol G., Zack J.A., Waring A.J., Yang O.O., and Lehrer R.I. Retrocyclin: a primate peptide that protects cells from infection by T- and M-tropic strains of HIV-1. Proc. Natl. Acad. Sci. USA 99 (2002) 1813-1818
7. Diamond G., Zasloff M., Eck H., Brasseur M., Maloy W.L., and Bevins C.L. Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA. Proc. Natl. Acad. Sci. USA 88 (1991) 3952-3956
8. Tang Y.Q., and Selsted M.E. Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils. J. Biol. Chem. 268 (1993) 6649-6653
9. Schonwetter B.S., Stolzenberg E.D., and Zasloff M.A. Epithelial antibiotics induced at sites of inflammation. Science 267 (1995) 1645-1648
10. Bensch K.W., Raida M., Magert H.J., Schulz-Knappe P., and Forssmann W.G. hBD-1: a novel beta-defensin from human plasma. FEBS Lett. 368 (1995) 331-335
11. Garcia J.R., Jaumann F., Schulz S., Krause A., Rodriguez-Jimenez J., Forssmann U., Adermann K., Kluver E., Vogelmeier C., Becker D., Hedrich R., Forssmann W.G., and Bals R. Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306 (2001) 257-264
12. Harder J., Bartels J., Christophers E., and Schroder J.M. A peptide antibiotic from human skin. Nature 387 (1997) 861
13. Harder J., Bartels J., Christophers E., and Schroder J.M. Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic. J. Biol. Chem. 276 (2001) 5707-5713
14. Alp S., Skrygean M., Schlottmann R., Kreuter A., Otte J.M., Schmidt W.E., Brockmeyer N.H., and Bastian A. Expression of beta-defensin 1 and 2 in nasal epithelial cells and alveolar macrophages from HIV-infected patients. Eur. J. Med. Res. 10 (2005) 1-6
15. Zilbauer M., Dorrell N., Boughan P.K., Harris A., Wren B.W., Klein N.J., and Bajaj-Elliott M. Intestinal innate immunity to Campylobacter jejuni results in induction of bactericidal human beta-defensins 2 and 3. Infect. Immun. 73 (2005) 7281-7289
16. Abiko Y., Saitoh M., Nishimura M., Yamazaki M., Sawamura D., and Kaku T. Role of beta-defensins in oral epithelial health and disease. Med. Mol. Morphol. 40 (2007) 179-184
17. Patil A.A., Cai Y., Sang Y., Blecha F., and Zhang G. Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract. Physiol. Genomics 23 (2007) 5-17
18. Zimmermann G.R., Legault P., Selsted M.E., and Pardi A. Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins. Biochemistry 34 (1995) 13663-13671
19. Hoover D.M., Chertov O., and Lubkowski J. The structure of human beta-defensin-1: new insights into structural properties of beta-defensins. J. Biol. Chem. 276 (2001) 39021-39026
20. Bauer F., Schweimer K., Kluver E., Conejo-Garcia J.R., Forssmann W.G., Rosch P., Adermann K., and Sticht H. Structure determination of human and murine beta-defensins reveals structural conservation in the absence of significant sequence similarity. Protein Sci. 10 (2001) 2470-2479
21. Hoover D.M., Rajashankar K.R., Blumenthal R., Puri A., Oppenheim J.J., Chertov O., and Lubkowski J. The structure of human beta-defensin-2 shows evidence of higher order oligomerization. J. Biol. Chem. 275 (2000) 32911-32918
22. Sawai M.V., Jia H.P., Liu L., Aseyev V., Wiencek J.M., McCray Jr. P.B., Ganz T., Kearney W.R., and Tack B.F. The NMR structure of human beta-defensin-2 reveals a novel alpha-helical segment. Biochemistry 40 (2001) 3810-3816
23. Schibli D.J., Hunter H.N., Aseyev V., Starner T.D., Wiencek J.M., McCray Jr. P.B., Tack B.F., and Vogel H.J. The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus. J. Biol. Chem. 277 (2002) 8279-8289
24. Landon C., Thouzeau C., Labbe H., Bulet P., and Vovelle F. Solution structure of spheniscin, a beta-defensin from the penguin stomach. J. Biol. Chem. 279 (2004) 30433-30439
25. White S.H., Wimley W.C., and Selsted M.E. Structure, function, and membrane integration of defensins. Curr. Opin. Struct. Biol. 5 (1995) 521-527
26. Brogden K.A. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nat. Rev. Microbiol. 3 (2005) 238-250
27. Yang D., Chertov O., Bykovskaia S.N., Chen Q., Buffo M.J., Shogan J., Anderson M., Schroder J.M., Wang J.M., Howard O.M., and Oppenheim J.J. Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286 (1999) 525-528
28. Pazgier M., Prahl A., Hoover D.M., and Lubkowski J. Studies of the biological properties of human beta-defensin 1. J. Biol. Chem. 282 (2007) 1819-1829
29. Taylor K., Clarke D.J., McCullough B., Chin W., Seo E., Yang D., Oppenheim J., Uhrin D., Govan J.R., Campopiano D.J., MacMillan D., Barran P., and Dorin J.R. Analysis and separation of residues important for the chemoattractant and antimicrobial activities of beta-defensin 3. J. Biol. Chem. 283 (2008) 6631-6639
30. Morgera F., Antcheva N., Pacor S., Quaroni L., Berti F., Vaccari L., and Tossi A. Structuring and interactions of human beta-defensins 2 and 3 with model membranes. J. Pept. Sci. (2007) 518-523
31. Krishnakumari V., and Nagaraj R. Interaction of antibacterial peptides spanning the carboxy-terminal region of human beta-defensins 1-3 with phospholipids at the air-water interface and inner membrane of E. coli. Peptides 29 (2008) 7-14
32. Andersson E., Rydengard V., Sonesson A., Morgelin M., Bjorck L., and Schmidtchen A. Antimicrobial activities of heparin-binding peptides. Eur. J. Biochem. 271 (2004) 1219-1226
33. Pristovsek P., and Kidric J. Solution structure of polymyxins B and E and effect of binding to lipopolysaccharide: an NMR and molecular modeling study. J. Med. Chem. 42 (1999) 4604-4613
34. Bhunia A., Domadia P.N., and Bhattacharjya S. Structural and thermodynamic analyses of the interaction between melittin and lipopolysaccharide. Biochim. et Biophys. Acta 1768 (2007) 3282-3291
35. T. Vargues, G.J. Morrison, E.S. Seo, D.J. Clarke, H.L. Fielder, J. Bennani, U. Pathania, F. Kilanowski, J.R. Dorin, J.R.W. Govan, L. Mackay, D. Uhrín, D.J. Campopiano, Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli, Prot. Pept. Lett., in press.
36. Studier F.W. Protein production by autoinduction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207-234
37. He F., Hendrickson C.L., and Marshall A.G. Unequivocal determination of metal atom oxidation state in naked heme proteins: Fe(III)myoglobin, Fe(III)cytochrome c, Fe(III)cytochrome b5, and Fe(III)cytochrome b5 L47R. J. Am. Soc. Mass Spectrom. 11 (2000) 120-126
38. Hwang T.L., and Shaka A.J. Water suppression that works. Excitation sculpting using arbitrary waveforms and pulsed field gradients. J. Magn. Reson. Series A 112 (1995) 275-279
39. Piotto M., Saudek V., and Sklenar V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J. Biomol. NMR 2 (1992) 661-665
40. Data were processed [in part] using the Azara suite of programs, provided by Wayne Boucher and the Department of Biochemistry, University of Cambridge. The code may be obtained via anonymous ftp to www.bio.cam.ac.uk in the directory ∼ftp/pub/azara.
41. Vranken W.F., Boucher W., Stevens T.J., Fogh R.H., Pajon A., Llinas M., Ulrich E.L., Markley J.L., Ionides J., and Laue E.D. The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59 (2005) 687-696
42. Wüthrich K. NMR of Proteins and Nucleic Acids (1986), Wiley, New York