An optimized procedure for efficient phage display of antibody fragments with a low folding efficiency

Protein Expression and Purification

Volumn 65, Issue 2, 2009, Pages 148-153

  Kuba, Hiroyoshi ^a   Furukawa, Koji ^a,b  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Affinity maturation; Biopanning; Expression; Fab; Phage display; Stability

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; PEPTIDE LIBRARY;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN FOLDING;

CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC VECTORS; IMMUNOGLOBULIN FAB FRAGMENTS; PEPTIDE LIBRARY; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); FILAMENTOUS BACTERIOPHAGE;

EID: 61749089697     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.01.016     Document Type: Article

References (10)

1. Porter R.R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem. J. 73 (1959) 119-126
2. Hoogenboom H.R., Griffiths A.D., Johnson K.S., Chiswell D.J., Hudson P., and Winter G. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19 (1991) 4133-4137
3. Barbas III C.F., Kang A.S., Lerner R.A., and Benkovic S.J. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. USA 88 (1991) 7978-7982
4. Winter G., Griffiths A.D., Hawkins R.E., and Hoogenboom H.R. Making antibodies by phage display technology. Annu. Rev. Immunol. 12 (1994) 433-455
5. Kuba H., Furukawa A., Okajima T., and Furukawa K. Efficient bacterial production of functional antibody fragments using a phagemid vector. Protein Expr. Purif. 58 (2008) 292-300
6. Furukawa K., Akasako-Furukawa A., Shirai H., Nakamura H., and Azuma T. Junctional amino acids determine the maturation pathway of an antibody. Immunity 11 (1999) 329-338
7. Furukawa K., Shimizu T., Murakami A., Kono R., Nakagawa M., Sagawa T., Yamato I., and Azuma T. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44 (2007) 2436-2445
8. Furukawa K., Shirai H., Azuma T., and Nakamura H. A role of the third complementarity-determining region in the affinity maturation of an antibody. J. Biol. Chem. 276 (2001) 27622-27628
9. Bradbury A.R.M., and Marks J.D. Phage antibody libraries, in Phage Display. In: Clackson T., and Lowman H.B. (Eds) (2004), Oxford University Press, New York 243-288
10. Yang W.P., Green K., Pinz-Sweeney S., Briones A.T., Burton D.R., and Barbas III C.F. CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J. Mol. Biol. 254 (1995) 392-403