Hepatic perturbations provoked by azathioprine: A paradigm to rationalize the cytoprotective potential of Ficus hispida Linn

Toxicology Mechanisms and Methods

Volumn 19, Issue 2, 2009, Pages 129-134

  Shanmugarajan, T S ^a   Devaki, T ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

Azathioprine; Ficus hispida; Hepatotoxicity; Mitochondria; Oxidant stress

Indexed keywords

ADENOSINE TRIPHOSPHATASE (CALCIUM); ADENOSINE TRIPHOSPHATASE (MAGNESIUM); ADENOSINE TRIPHOSPHATE; ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; AZATHIOPRINE; FICUS HISPIDA LEAF EXTRACT; GAMMA GLUTAMYLTRANSFERASE; ISOCITRATE DEHYDROGENASE; LACTATE DEHYDROGENASE; LIVER ENZYME; MITOCHONDRIAL ENZYME; PLANT EXTRACT; SUCCINATE DEHYDROGENASE; THIOL; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL NUCLEUS; CELL PROTECTION; CITRIC ACID CYCLE; CONTROLLED STUDY; DRUG EFFECT; DRUG ISOLATION; FICUS; FICUS HISPIDA; LIVER FUNCTION TEST; LIVER MITOCHONDRION; LIVER NECROSIS; LIVER PROTECTION; LIVER TOXICITY; MALE; NONHUMAN; OXIDATIVE STRESS; PLANT LEAF; PRIORITY JOURNAL; RAT; SINGLE DRUG DOSE; TRANSMISSION ELECTRON MICROSCOPY; TREATMENT DURATION;

ADENINE NUCLEOTIDES; ANIMALS; ANTIMETABOLITES; AZATHIOPRINE; CYTOPROTECTION; FICUS; LIVER; MALE; MITOCHONDRIA, LIVER; PLANT EXTRACTS; PLANT LEAVES; RANDOM ALLOCATION; RATS; RATS, WISTAR; SULFHYDRYL COMPOUNDS;

FICUS HISPIDA; RATTUS;

EID: 61649085653     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.1080/15376510802322489     Document Type: Article

References (32)

1. Amin, A., and Hamza, A. A. 2005. Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci. 77:266-278.
2. Galindo, M. F., Jordán, J., González-García, C., and Ceña, V. 2003. Reactive oxygen species induce swelling and cytochrome c release but not transmembrane depolarization in isolated rat brain mitochondria. Br. J. Pharmacol. 139;797-804.
3. 2+-ATPase from erythrocyte membranes. Biochim. Biophys. Acta 728:281-288.
4. Jeong, H. G. 1999. Inhibition of cytochrome P450 2E1 expression by oleanolic acid: hepatoprotective effects against carbon tetrachloride-induced hepatic injury. Toxicol. Lett. 105;215-222.
5. +-ATPase: enzyme sources, preparative problems, and preparation from mammalian kidney. Methods Enzymol. 156:29-43.
6. King, J. 1965a. The transferases-alanine and aspartate transaminases. In: Van, D. (Ed.), Practical Clinical Enzymology, Nostrand Company Limited, London, pp. 121-138.
7. King, J. 1965b. The hydrolases - acid and alkaline phosphatases. In: Van, D. (Ed.), Practical Clinical Enzymology, Nostrand Company Limited, London, pp. 199-208.
8. King, J. 1965c. The dehydrogenases or oxidoreductases-lactacte dehydrogenase. In: Van, D. (Ed.), Practical Clinical Enzymology, Nostrand Company Limited, London, pp. 83-93.
9. King, J. 1965d. Isocitrate dehydrogenase. In: Van, D. (Ed.), Practical Clinical Enzymology, Nostrand Company Limited, London, p. 363.
10. Lee, A. U., and Farrell, G. C. 2001. Mechanism of azathioprine-induced injury to hepatocytes: roles of glutathione depletion and mitochondrial injury. J. Hepatol. 35:756-764.
11. Li, C. R., Zhou, Z., Lin, R. X., Zhu, D., Sun, Y. N., Tian, L. L., Li, L., Gao, Y., and Wang, S. Q. 2007. Beta-sitosterol decreases irradiation-induced thymocyte early damage by regulation of the intracellular redox balance and maintenance of mitochondrial membrane stability. J. Cell Biochem. 102:748-758.
12. Liu, Y., and Wang, M. W. 2008. Botanical drugs: challenges and opportunities: contribution to Linnaeus Memorial Symposium 2007. Life Sci. 82:445-449.
13. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
14. Maltzman, J. S., and Koretzky, G. A. 2003. Azathioprine: old drug new actions. J. Clin. Invest. 111:1122-1124.
15. Mandal, S. C., Saraswathi, B., Kumar, C. K., Lakshmi, S. M., and Maiti, B. C. 2000. Protective effect of leaf extract of Ficus hispida Linn. against paracetamol-induced hepatotoxicity in rats. Phytother. Res. 14:457-459.
16. Mehler, A. H., Kornberg, A., Grisolia, S., and Ochoa, S. 1948. The enzymatic mechanism of oxidation-reductions between malate or isocitrate and pyruvate. J. Biol. Chem. 174:961-977.
17. Menor, C., Fernández-Moreno, M. D., Fueyo, J. A., Escribano, O., Olleros, T., Arriaza, E., Cara, C., Lorusso, M., Di Paola, M., Román, I. D., and Guijarro, L. G. 2004. Azathioprine acts upon rat hepatocyte mitochondria and stress-activated protein kinases leading to necrosis: protective role of N-acetyl-L-cysteine. J. Pharmacol. Exp. Ther. 311:668-676.
18. 2+-ATPase activities in normal, regenerating and malignant cells. Biochim. Biophys. Acta 684:67-74.
19. Oliveira, F. A., Chaves, M. H., Almeida, F. R., Lima, R. C., Silva, R. M., Maia, J. L., Brito, G. A., Santos, F. A., and Rao, V. S. 2005. Protective effect of alpha- and beta-amyrin, a triterpene mixture from Protium heptaphyllum (Aubl.) March. trunk wood resin, against acetaminophen-induced liver injury in mice. J. Ethnopharmacol. 98:103-108.
20. Patel, A. A., Swerlick, R. A., and McCall, C. O. 2006. Azathioprine in dermatology: the past, the present, and the future. J. Am. Acad. Dermatol. 55:369-389.
21. Raghavendran, H. R. B., Sathivel, A., and Devaki, T. 2005. Antioxidant effect of Sargassum polycystum (Phaeophyceae) against acetaminophen induced changes in hepatic mitochondrial enzymes during toxic hepatitis. Chemosphere 61:276-281.
22. Raghavendran, H. B., Sathivel, A., and Devaki, T. 2006. Defensive nature of Sargassum polycystum (Brown alga) against acetaminophen-induced toxic hepatitis in rats: role of drug metabolizing microsomal enzyme system, tumor necrosis factor-alpha and fate of liver cell structural integrity. World J. Gastroenterol. 12:3829-3834.
23. Raza, M., Ahmad, M., Gado, A., and Al-Shabanah, O. A. 2003. A comparison of hepatoprotective activities of aminoguanidine and N-acetylcysteine in rat against the toxic damage induced by azathioprine. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 134;451-456.
24. Reed, L. J., and Mukherjee, B. B. 1969. α-Ketoglutarate dehydrogenase complex from Escherichia coli. In: Lowenstein, J. M. (Ed.), Methods in Enzymology, Vol. 13, Academic Press, London, pp. 55-61.
25. Rosalki, S. B., and Rau, D. 1972. Serum-glutamyl transpeptidase activity in alcoholism. Clin. Chim. Acta 39:41-47.
26. Sedlak, J., and Lindsay, R. H. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal. Biochem. 25:192-205.
27. Shanmugarajan, T. S., Arunsundar, M., Somasundaram, I., Sivaraman, D., Krishnakumar, E., and Ravichandran, V. 2008. Ameliorative effect of Ficus hispida Linn. leaf extract on cyclophosphamide-induced oxidative hepatic injury in rats. J. Pharmacol. Toxicol. In press.
28. Slater, E. C., and Borner, W. D. 1952. The effect of fluoride on the succinic oxidase system. Biochem. J. 52:185-196.
29. Tang, X. H., Gao, J., Fang, F., Chen, J., Xu, L. Z., Zhao, X. N., and Xu, Q. 2005. Hepatoprotection of oleanolic acid is related to its inhibition on mitochondrial permeability transition. Am. J. Chin. Med. 33:627-637.
30. Tapner, M. J., Jones, B. E., Wu, W. M., and Farrell, G. C. 2004. Toxicity of low dose azathioprine and 6-mercaptopurine in rat hepatocytes. Roles of xanthine oxidase and mitochondrial injury. J. Hepatol. 40:454-463.
31. Williamson, J. R., and Corkey, B. E. 1969. Assays of intermediates of the citric acid cycle and related compounds by fluorometric enzymes methods. In: Lowenstein, J. M., (Ed.), Methods in Enzymology, Academic Press, New York, pp. 488-491.
32. Yokota, J., Takuma, D., Hamada, A., Onogawa, M., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S., and Nishioka, Y. 2006. Scavenging of reactive oxygen species by Eriobotrya japonica seed extract. Biol. Pharm. Bull. 29:467-471.