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Initially, we named the 42 AA C-terminal domain DEAD-Acidic- 2. However, to avoid confusion with the individual subdomain names, we have chosen to rename the 42 AA sequence Fragment-2.
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A brief description of AFM tip interaction and potential imaging artifacts are warranted here. We were able to ascertain that tip interactions were not influencing our imaging via the following observations. First, with regard to potential scanning artifacts, we see no evidence of protein piling up at the edges of a scan box, which is a typical test to determine if the AFM tip is scraping material from the surface. This is tested by scanning a small scan area for several scans and then subsequently scanning a larger area. If material is being removed from the surface by the tip, then the material will pile up at the edges of the small scan (i.e, the snow plow effect) and this mark will be observed in the larger scan area. This phenomenon is always tested for in each experiment. Second, when we first introduce the polypeptides, we also halt imaging for 5 minutes (with the tip raised) to lessen the chance that the tip is influencing polypeptide-surface binding. Third, we als
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A brief description of AFM tip interaction and potential imaging artifacts are warranted here. We were able to ascertain that tip interactions were not influencing our imaging via the following observations. First, with regard to potential scanning artifacts, we see no evidence of protein "piling up" at the edges of a scan box, which is a typical test to determine if the AFM tip is scraping material from the surface. This is tested by scanning a small scan area for several scans and then subsequently scanning a larger area. If material is being removed from the surface by the tip, then the material will pile up at the edges of the small scan (i.e., the snow plow effect) and this mark will be observed in the larger scan area. This phenomenon is always tested for in each experiment. Second, when we first introduce the polypeptides, we also halt imaging for 5 minutes (with the tip raised) to lessen the chance that the tip is influencing polypeptide-surface binding. Third, we also image with the lightest contact force possible by adjusting the set point frequently. The reason for using contact mode over tapping mode is that the image capture rate in tapping mode is too slow to quantify these step kinetics, and we do not see evidence of "snow plow" artifacts in contact mode.
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The energy required to create a kink in sparingly soluble minerals such as calcite is relatively high (E/kT ≈ 2.4)26.27 which implies that kink nucleation is likely the rate-limiting step for step growth, giving credence to this proposed scenario
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26.27 which implies that kink nucleation is likely the rate-limiting step for step growth, giving credence to this proposed scenario.
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