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Volumn 19, Issue 6, 2009, Pages 1815-1817

Lithospermic acid derivatives from Lithospermum erythrorhizon increased expression of serine palmitoyltransferase in human HaCaT cells

Author keywords

Lithospermic acid; Lithospermum erythrorhizon; Normal human keratinocyte cells (HaCaT cells); Serine palmitoyltransferase (SPT)

Indexed keywords

9' METHYLLITHOSPERMATE; LITHOSPERMIC ACID; LITHOSPERMUM ERYTHRORHIZON EXTRACT; MESSENGER RNA; NICOTINAMIDE; PLANT EXTRACT; SERINE PALMITOYLTRANSFERASE; UNCLASSIFIED DRUG;

EID: 61449218775     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.01.052     Document Type: Article
Times cited : (20)

References (25)
  • 14
    • 61449166217 scopus 로고    scopus 로고
    • note
    • R = 42 min).
  • 15
    • 61449200752 scopus 로고    scopus 로고
    • note
    • 13C NMR, see Table S1 (Supplementary data).
  • 18
    • 0021061819 scopus 로고    scopus 로고
    • note
    • 2. Cells were harvested and analyzed 9 or 24 h after each treatment by reverse transcriptase-polymerase chain reaction (RT-PCR) or Western blot analysis. The cytotoxic activity of compounds against HaCaT cells was examined by a MTT method (Mosmann, T. J. Immunol. Method 1983, 65, 55).
  • 19
    • 61449175217 scopus 로고    scopus 로고
    • note
    • 11 Statistical analysis. The statistical analyses were performed using the SPSS program (SPSS 12.0). One sample t-test was used to examine the difference between groups. p Values of 0.05 were considered to be statistically significant.
  • 20
    • 61449259325 scopus 로고    scopus 로고
    • note
    • 12,13
  • 21
    • 0037087402 scopus 로고    scopus 로고
    • note
    • NF-κB reporter gene assay. Cells were plated at in 12-well plate and transfected at 70% confluency. A dual-luciferase reporter assay system (Promega, Madison, WI) was used to determine promoter activity. Briefly, cells were transiently transfected with pNF-κB-luciferase plasmid and phRL-SV plasmid using the Hillymax reagent (Dojindo Molecular Technologies, Gaitherburg, MD). The cells were then incubated in culture medium without serum for 18 h. The firefly and hRenilla luciferase activities in the cell lysates were measured using a luminometer (LB941, Berthold Technologies, Bad Wildbad, Germany). The relative luciferase activity was calculated by normalizing the promoter-driven firefly luciferase activity to the hRenilla luciferase activity (Majumdar, S.; Lamothe, B.; Aggarwal, B. B. J. Immunol. 2002, 168, 2644).
  • 24
    • 61449192107 scopus 로고    scopus 로고
    • note
    • 22


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.