Clenbuterol storage stability in the bovine urine and liver samples used for European official control in the Azores Islands (Portugal)

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 3, 2009, Pages 910-914

  Pinheiro, Isabel ^a   Jesuino, Bruno ^b   Barbosa, Jorge ^b   Ferreira, Humberto ^c   Ramos, Fernando ^d   Matos, José ^e   Da Silveira, Maria Irene Noronha ^d  

^a Direcção Regional do Desenvolvimento Agrário   (Portugal)

^b Laboratorio Nacional de Investigacao Veterinaria   (Portugal)

^c RESEARCH INSTITUTE FOR MEDICINES IMED ULISBOA   (Portugal)

^d UNIVERSITY OF COIMBRA   (Portugal)

^e UNIVERSITY OF THE AZORES   (Portugal)

Author keywords

Clenbuterol; GC MS; Liver; Stability; Urine

Indexed keywords

ANIMALIA; BOS; BOVINAE;

BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CLENBUTEROL; GROWTH PROMOTOR;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; DRUG STABILITY; FREEZING; HEAT; LIVER; MASS FRAGMENTOGRAPHY; METABOLISM; PORTUGAL; SENSITIVITY AND SPECIFICITY; URINE;

ADRENERGIC BETA-AGONISTS; ANIMALS; CATTLE; CLENBUTEROL; DRUG STABILITY; FREEZING; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GROWTH SUBSTANCES; HOT TEMPERATURE; LIVER; PORTUGAL; SENSITIVITY AND SPECIFICITY;

EID: 61449191869     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf802995e     Document Type: Article

References (25)

1. Cristino, A.; Ramos, F.; Silveira, M. I. N. Control of the illegal use of clenbuterol in bovine production. J. Pharm. Biomed. Anal. 2003, 32, 311-316.
2. Hanrahan, J. P. Beta-agonists and Their Effects on Animal Growth and Carcass Quality; Elsevier: London, 1987.
3. Council Regulation 2377/90/EEC of 26 June 1990 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off. J. Eur. Union 1990, L224, 1-8.
4. Brambilla, G.; Cenci, T.; Franconi, F.; Galarini, R.; Macri, A.; Rondoni, F.; Strozzi, M.; Loizzo, A. Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy. Toxicol. Lett. 2000, 114, 47-53.
5. Garay, J. B.; Jiménez, J. F. H.; Jiménez, M. L.; Sebastian, M. V.; Matesanz, J. P.; Moreno, P. M.; Galiana, J. R. Intoxicación por clenbuterol: datos clínicos y analíticos de un brote epidémico en Móstoles, Madrid. Rev. Clin. Esp. 1997, 197, 92-95.
6. Martinez-Navarro, J. F. Food poisoning related to consumption of illicit β-agonist in liver. Lancet 1990, 336, 1311.
7. Pulce, C.; Lamaison, D.; Keck, G.; Bostvironnois, C.; Nicolas, J.; Descotes, J. Collective human food poisonings by clenbuterol residues in veal liver. Vet. Hum. Toxicol. 1991, 33, 480-481.
8. Sporano, V.; Grasso, L.; Esposito, M.; Oliviero, G.; Brambilla, G.; Loizzo, A. Clenbuterol residues in non-liver containing meat as a cause of collective food poisoning. Vet. Hum. Toxicol. 1998, 40, 141-143.
9. Ramos, F.; Silveira, M. I. N.; Silva, J. M.; Barbosa, J.; Cruz, C.; Martins, J.; Neves, C.; Alves, C. Proposed guidelines for clenbuterol food poisoning. Am. J. Med. 2004, 117, 362.
10. Barbosa, J.; Cruz, C.; Martins, J.; Silva, J. M.; Neves, C.; Alves, C.; Ramos, F.; Silveira, M. I. N. Food poisoning by clenbuterol in Portugal. Food Addit. Contam. 2005, 22, 563-566.
11. Commission Decision 2002/657/CE of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off. J. Eur. Commun. 2002, L221, 8-35.
12. Gude, Th.; Preiss-Weigert, A.; van Ginkel, L. A.; Dirscherl, C. Reference materials-bcr information-the certification of the clenbuterol and salbutamol concentrations in lyophilized bovine urine-bcr-502-504. In Cunningham, N. F., Heitzman, R. J. (Report), European Commission EUR 19452/EN; Brussels, 2001.
13. Gigosos, P. G.; Sampayo, C. F.; Abuín, C. F.; Sáez, A. C.; Belda, B. I. V. Effects of frozen storage on the recovery of clenbuterol from urine samples for official control. Food Addit. Contam. 2002, 19, 1010-1014.
14. Pinheiro, I.; Jesuíno, B.; Barbosa, J.; Ramos, F.; Matos, J.; Silveira, M. I. N. Production of "in house" reference materials for ELISA screening of bovine urine and liver samples for clenbuterol. Accred. Qual. Assur. 2008, 13, 299-304.
15. Gaspar, P.; van-Vyncht, G.; Scippo, M. L.; Maghuin-Rogister, G.; Kramer, G. N.; Gawlik, B. M.; Linsinger, T.; Lamberty, A.; van-Ginkel, L. A.; Dirscherl, C. Certification of the clembuterol mass concentration in lyophilised bovine liver reference materials with indicative information on the salbutamol and terbutaline mass concentrations, BCR-648 (b-agonist-free) and BCR-649 (b-agonist-positive). EUR 20526/EN;Brussels, 2002.
16. Gude, T.; Schmädicke, I.; Rann, S.; Benesch-Girke, L.; Hashem, A.; Preiss, A.; Jülicher, B. The influence of the choice and the pretreatment of suitable tissue samples on the results of residue analyses using clenbuterol as an example. In Residues of veterinary drugs in food. Proceedings of the EuroResidue III conference; Haagsma, N., Ruiter, A., Eds.; Veldhoven: 1996; pp 157-161.
17. Rose, M. D.; Shearer, G.; Farrington, W. H. H. The thermal stability and effect of cooking on veterinary drug residues in food: clenbuterol. Food Addit. Contam. 1995, 12, 67-76.
18. Ramos, F.; Castilho, M. C.; Silveira, M. I. N. Estudo dos efeitos do processamento culinário sobre os resíduos de clembuterol e salbutamol em fígado de vitela. Rev. Port. Farm. 2000, L:3, 117-123.
19. Barone, R. Anatomie Comparée des Mamifères Domestique. Tome troisième. Splanchnologie I. Appareil digestif. Appareil respiratoire, 3rd ed.; Éditions Vogot: Paris, 1997.
20. Montrade, M.-P.; le-Bizec, B.; Monteau, F.; Siliarte, B.; André, F. Multi-residue analysis for β-agonistic drugs in urine of meatproducing animals by gas chromatography-mass spectrometry. Anal. Chim. Acta 1993, 275, 253-268.
21. Heitzman, R. J. Veterinary drug residues-Residues in food producing animals and their products-Reference materials and methods. CEC Report EUR 15127-EN, 2nd ed.; published on behalf of Commission of the European Communities, Blackwell Scientific Publications: Brussels, 1994.
22. Boenish, B.; Quirke, J. F. Safety assessment of β-agonists. In Kuiper, H. A., Hoogenboom, L. A. P., In Vitro Toxicological Studies and Real Time Analysis of Residues in Food-FLAIR Concerted Action No 8-Proceedings of the Workshops held in Ghent, May 22-24, 1992 and Thessaloniki, October 30-31, 1992. Wageningen, 1992.
23. Smith, D. J. The pharmacokinetics, metabolism, and tissue residues of β-adrenergic agonists in livestock. J. Anim. Sci. 1998, 76, 173-194.
24. Olsen, M. M.; Peron, G.; Godzdanker, R. Examination of Conventional Freeze Thaw Processing on Solute Distribution. National Biotechnology Conference, Boston (MD), 2006.
25. Cao, E.; Chen, Y.; Cui, Z.; Foster, P. R. Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnol. Bioeng. 2003, 82 (6), 684-690.