Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions

Biotechnology and Bioengineering

Volumn 82, Issue 6, 2003, Pages 684-690

  Cao, Enhong ^a   Chen, Yahuei ^a   Cui, Zhanfeng ^a   Foster, Peter R ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b SCOTTISH NATIONAL BLOOD TRANSFUSION SERVICE   (United Kingdom)

Author keywords

Buffer type; Cryomicroscope; Freeze denaturation; Freezing rate; Lactate dehydrogenase; Protein freezing; Thawing rate

Indexed keywords

CRYOGENICS; CRYSTALLIZATION; FREEZING; PH EFFECTS; SOLUTIONS; THAWING;

DENATURATION;

PROTEINS;

ALCOHOL DEHYDROGENASE; BUFFER; CATALASE; ICE; LACTATE DEHYDROGENASE; POTASSIUM DIHYDROGEN PHOSPHATE;

AQUEOUS SOLUTION; ARTICLE; CRYSTALLIZATION; ENZYME ACTIVITY; FREEZING; LIQUID; PH; PROTEIN DENATURATION; SHEAR STRESS; SURFACE PROPERTY; TENSION; THAWING;

ALCOHOL DEHYDROGENASE; BUFFERS; CATALASE; CRYOPRESERVATION; DRUG STABILITY; DRUG STORAGE; ENZYME ACTIVATION; ENZYME STABILITY; FREEZING; HEAT; L-LACTATE DEHYDROGENASE; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEINS; SOLUTIONS; TEMPERATURE; WATER;

EID: 0038632059     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10612     Document Type: Article

References (20)

1. Anchordoquy TJ, Carpenter JF. 1996. Polymer protect lactate dehydrogenase during freezing-drying by inhibiting dissociation in the frozen state. Arch Biochem Biophys 332:231-238.
2. Arakawa T, Prestrelsk SJ, Kenney WC, Carpenter JF. 1993. Factors affecting short-term and long-term stabilities of proteins. Adv Drug Del Rev 10:1-28.
3. Chang BS, Kendrick BS, Carpenter JF. 1996. Surface-induced denaturation of proteins during freezing and its inhibition by surfactant. J Pharm Sci 85:1325-1330.
4. Chilson OP, Costello LA, Kaplan NO. 1965. Biochemistry 4:271-281.
5. Eckhardt BM. Oeswein JQ, Bewley TA. 1991. Effect of freezing on aggregation of human growth hormone. Pharm Res 8:1360-1364.
6. Fishbein WN, Winkert JW. 1978. Parameters of biological freezing damage in simple solutions: catalase. II. Demonstration of an optimumrecovery cooling-rate curve in a membraneless system. Cryobiology 15:168-177.
7. Franks F. 1985. Biophysics and biochemistry at low temperature. London: Cambridge University Press.
8. Fransen GJ, Salemilk PJ, Cormmelin DJA. 1986. Critical parameters in freezing of liposomes. Int J Pharm 33:27-35.
9. Izutsu K, Yoshioka S, Terao T. 1994. Stabilizing effect of amphiphilic excipients on the freezing-thawing and freezing-drying of lactate dehydrogenase. Biotechnol Bioeng 43:1102-1107.
10. Jiang S, Nail SL. 1998. Effect of process conditions on recovery of protein activity after freezing and freezing-drying. Eur J Pharm Biopharm 45:249-257.
11. Larsen SS. 1973. Studies on stability of drugs in frozen systems. VI. The effect of freezing upon pH for buffered aqueous solutions. Arch Pharm Chem Sci Ed 1:41-53.
12. Murase N, Franks F. 1989. Salt precipitation during the freeze-concentration of phosphate buffer solutions. Biophys Chem 34:293-230.
13. Nema S, Avis KE. 1993. Freeze-thaw studies of a model protein, lactate dehydrogenase, in the presence of cryoprotectants. J Parenter Sci Technol 47:76-83.
14. Orii Y, Morita M. 1977. Measurement of the pH of frozen buffer solutions by using pH indicators. J Biochem 81:163-168.
15. 125I]human growth hormone in response to freezing and thawing. Endocrinology 92: 1795-1798.
16. Shikama K, Yamazaki I. 1961. Denaturation of catalase by freezing and thawing. Nature 190:83-84.
17. Soliman FS, van de Berg L. 1971. Factors affecting freezing damage of lactic dehydrogenase. Cryobiology 8:73-78.
18. Strambini GB, Gabellieri E. 1996. Protein in frozen solutions: evidence of ice-induced partial unfolding. Biophys J 70:971-976.
19. Tamiya T, Okahashi N, Sakuma R, Aoyama T, Akahane T, Matsumoto JJ. 1985. Freeze denaturation of enzymes and its prevention with additives. Cryobiology 22:446-456.
20. 2O. Arch Biochem Biophys 81: 319-329.