EST markers in evergreen azalea: Development and use for classifications compared to AFLP, microsatellite and morphological data

Acta Horticulturae

Volumn 763, Issue , 2007, Pages 107-115

  De Keyser, Ellen ^a   De Riek, Jan ^a   Handa, Takashi ^b   Scariot, Valentina ^c  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c UNIVERSITY OF TURIN   (Italy)

Author keywords

CDNA libraries; Expressed sequence tag; Marker comparison; Marker development; Rhododendron spp

Indexed keywords

RHODODENDRON; RHODODENDRON SIMSII;

EID: 61449147135     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.2007.763.14     Document Type: Conference Paper

References (19)

1. Belaj, A., Satovic, Z., Cipriani, G., Baldoni, L., Testolin, R., Rallo, L. and Trujillo, I. 2003. Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theor. Appl. Genet. 107:736-744.
2. Chamberlain, D. F. and Hyam, R. 1998. The genus Rhododendron: a case study to test the value of various molecular techniques as measures of biodiversity. p. 441-448. In: A. Karp, P. G. Isaac and D. S. Ingram (eds.), Molecular Tools for Screening Biodiversity. Chapman & Hall, London.
3. Chamberlain, D. F. and Rae, S. J. 1990. A revision of Rhododendron IV Subgenus Tsutsusi. Edinburgh J. Bot. 47:89-200.
4. De Riek, J., Dendauw, J., Mertens, M., De Loose, M., Heursel, J. and Van Bockstaele, E. 1999. Validation of criteria for the selection of AFLP markers to assess the genetic variation of a breeders' collection of evergreen azaleas. Theor. Appl. Gen. 99:1155-1165.
5. De Schepper, S., Leus, L., Mertens, M., Van Bockstaele, E., Debergh, P. and Heursel, J. 2001. Flow cytometric analysis of ploidv in Rhododendron (subgenus Tsutsusi). HortScience 36:125-127.
6. Dendauw, J., De Riek, J., Arens, P., Van Bockstaele, E., Vosman, B. and De Loose, M. 2001. Development of sequenced tagged microsatellite site (STMS) markers in azalea. Acta Hort. 546:193-197.
7. Dendauw, J., De Riek, J., De Loose, M. and Van Bockstaele, E. 2002. Identification of 33 Chinese Rhododendron species using mat K sequences and AFLP data. Acta Hort. 572:169-177.
8. Dunemann, F., Kahnau, R. and Stange, I. 1999. Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map. Theor. Appl. Genet. 98:1146-1155.
9. Excoffier, L., Smouse, P. and Quattro, J. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479-491.
10. Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44:223-270.
11. Kameyama, Y., Isagi, Y. and Nakagoshi, N. 2001. Patterns and levels of gene flow in Rhododendron metternichii var. hondoense revealed my microsatellite analysis. Mol. Ecol. 10:205-216.
12. Kron, K. A. 1997. Phylogenetic relationships of Rhododendroideae (Ericaceae). Amer. J. Bot. 84:973-980.
13. Kurashige, Y., Etoh, J. I., Handa, T., Takayanagi, K. and Yukawa, T. 2001. Sectional relationships in the genus Rhododendron (Ericaceae): evidence from mat K and trn K sequences. Plant. Syst. Evol. 228:1-14.
14. Kurashige, Y., Milne, M., Kobayashi, N., Handa, T., Takayanagi, K. and Yukawa, T. 1998. Investigation of sectional relationships in the genus Rhododendron (Ericaceae) based on mat K sequences. J. Jpn. Bot. 73:143-154.
15. Naito, K., Isagi, Y. and Nakagoshi, N. 1998. Isolation and characterization of microsatellites of Rhododendron metternichii Sieb. et Zucc. var. hondoense Nakai. Mol. Ecol. 7:927-928.
16. Pearson, W. R. and Lipman, D. J. 1988. PNAS. 85:2444-2448.
17. Scariot, V., De Keyser, E., Handa, T. and De Riek, J. 2007. Comparative study of the discriminating capacity and effectiveness in assessing genetic relationships of AFLP, STMS and EST markers in evergreen azaleas. Plant Breeding 126:207-212.
18. Schneider, S., Roessli, D. and Excoffier, L. 2000. Arlequin: a software for population genetics data analysis, Version 2.000. Genetics and Biometry Laboratory, Department of Anthropology, University of Geneva, Switzerland.
19. Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Homes, M., Fijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 21:4407-4414.