Improved resolution in the separation of monoclonal antibody isoforms using controlled pH gradients in IEX chromatography

American Biotechnology Laboratory

Volumn 27, Issue 1, 2009, Pages 10-12

  Tsonev, Latchezar I ^a   Hirsh, Allen G ^b  

^a Biomedical R and D   (United States)

^b BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 61449132539     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

1. Tsonev, L.; Hirsh, A. Theory and application of a novel ion exchange chromatographic technology using controlled pH gradients for separating proteins on anionic and cationic stationary phases. J. Chro-matogr. A 2008, 1200(2), 166-82.
2. Friedman, L.M.; Rinon, A.; Schechter, B.; Lyass, L.; Lavi, S.; Bacus, S.S.; Sela, M.; Yarden, Y. Synergistic down-regulation of receptor tyrosine kinases by combinations of mAbs: implications for cancer immunotherapy. Proc. Natl. Acad. Sci. USA 2005, 102(6), 91 5-20.
3. Tang, Y.; Lou, J.; Alpaugh, R.K.; Robinson, M.K. Regulation of antibody-dependent cellular cytotoxicity by IgG intrinsic and apparent affinity for target antigen. J. Immunol. 2007, 179,2815-23.
4. Schuster, M.; Jost, W.; Mudde, G.C.; Wiederkum, S.; Schwager, C; Janzek, E.; Altmann, F.; Stadlmann, J.; Stemmer, C.; Gorr, G. In vivo glyco-engineered antibody with improved lytic potential produced by an innovative non-mammalian expression system. Biotechnol ]. Jim 2007, 2(6), 700-8.
5. Stromea, S.E.; Sausvilleb, E.A.; Mann, D. A mechanistic perspective of monoclonal antibodies in cancer therapy beyond target-related effects. The Oncologist 2007, J 2(9), 1084-95.
6. Hirsh, A.G.; Tsonev, L.I. Application of well-controlled pH gradients at variable isocratic salt concentrations to IEX chromatography. Am. Biotech. Lab. 2008, 26(10) 16-7.