Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling

Cytometry Part A

Volumn 75, Issue 2, 2009, Pages 163-168

  Duhamel, Solange ^a,b,d   Gregori, Gerald ^a,b   Van Wambeke, France ^a,b   Nedoma, Jiří ^c  

^a AIX MARSEILLE UNIVERSITY   (France)

^b STATION DE RADIOASTRONOMIE DE NANÇAY   (France)

^c INSTITUTE OF HYDROBIOLOGY   (Czech Republic)

^d UNIVERSITY OF HAWAII   (United States)

Author keywords

Alkaline phosphatase; Elf phosphate; Heterotrophic bacteria; Kinetics

Indexed keywords

ALCOHOL DERIVATIVE; FRESH WATER; PHOSPHATASE;

AQUATIC ENVIRONMENT; CELL ACTIVITY; CELL FRACTIONATION; CELL LABELING; CELL LEVEL; CONFERENCE PAPER; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE; HYDROLYSIS; INCUBATION TIME; LAKE; MARINE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; TIME;

BACTERIA; FLOW CYTOMETRY; FRESH WATER; KINETICS; ORGANOPHOSPHORUS COMPOUNDS; PHOSPHORIC MONOESTER HYDROLASES; PHYTOPLANKTON; QUINAZOLINONES; SEAWATER; STAINING AND LABELING;

EID: 60849135117     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20686     Document Type: Conference Paper

References (25)

1. Harz M, Rosch P, Popp J. Vibrational spectroscopy-A powerful tool for the rapid identification of microbial cells at the single-cell level. Cytometry Part A 2009;75A: in press. DOI: 10.1002/cyto.a.20682 (this issue).
2. Zordan MD, Grafton MMG, Acharya G, Reece LM, Cooper CL, Aronson AI, Park K, Leary JF. Detection of pathogenic E. coli O157:H7 by a hybrid microfluidic SPR and molecular imaging cytometry device. Cytometry Part A 2009;75A:in press. DOI:10.1002/cyto.a.20692(thisissue).
3. Kortmann H, Blank LM, Schmid A. Single cell analysis reveals unexpected growth phenotype of S. cerevisiae. Cytometry Part A 2009;75A:in press. DOI: 10.1002/ cyto.a.20684 (this issue).
4. Want A, Thomas ORT, Kara B, Liddell J, Hewitt CJ. Studies related to antibody fragment (Fab) production in Escherichia coli W3110 fed-batch fermentation processes using multi-parameter flow cytometry. Cytometry Part A 2009;75A:in press. DOI:10.1002/cyto.a.20683 (thisissue).
5. Porro D, Vai M, Vanoni M, Alberghina L, Hatzis C. Analysis and modelling of growing budding yeast populations at the single cell level. Cytometry Part A 2009;75A:inpress. DOI: 10.1002/cyto.a.20689(thisissue).
6. Dyhrman S, Ammerman JW, Van Mooy B. Microbes and the marine phosphorus cycle. Oceanography 2007;20:110-116.
7. Karl DM. Phosphorus, the staff of life. Nature 2000;406:31-32.
8. Karl D, Björkman K. Dynamics of dissolved organic matter. In: Hansell Carlson, Editor. Biogeochemistry of Marine Dissolved Organic Matter. San Diego, CA: Academic Press; 2002. pp 249-366.
9. Hoppe H-G. Phosphatase in the sea. Hydrobiologia 2003;493:187-200.
10. Clark LL, Ingall ED, Benner R. Marine phosphorus is selectively remineralized. Nature 1998;393:426.
11. Gonzalez-Gil S, Keafer BA, Jovine RVM, Aguilera A, Lu S, Anderson DM. Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton. MarEcolProg Ser1998;164:21-35.
12. Labry C, Delmas D, Herbland A. Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters(BayofBiscay).JExpMarBiolEcol2005;318:213-225.
13. Dyhrman ST, Palenik B. Phosphate stress in cultures and field populations of the dinoflagellate Prorocentrum minimum detected by a single-cell alkaline phosphatase assay. Appl Environ Microbiol 1999;65:3205-3212.
14. Strojsova A, Vrba J. Phytoplankton extracellular phosphatases: Investigation using the enzyme labelled fluorescence (ELF) technique. Polish J Ecol2006;54:715-723.
15. Duhamel S, Gregori G, Van Wambeke F, Mauriac R, Nedoma J. A method for analysing phosphatase activity in aquatic bacteria at the single cell level using flow cytome-try. J Microbiol Methods 2008;75:269-278.
16. Dignum M, Hoogveld HL, Matthijs HCP, Laanbroek HJ, Pel R. Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry. FEMS Microbiol Ecol 2004;48:29-38.
17. Kopáček J, Brzáková M, Hejzlar J, Nedoma J, Porcal P, Vrba J. Nutrient cycling in a strongly acidified mesotrophic lake. Limnol Oceanogr 2004;49:1202-1213.
18. Vrba J, Kopáček J, Fott J. Long-term limnological research of the Bohemian Forest lakes and their recent status. Silva Gabreta 2000;4:7-28.
19. Strickland JDH, Parsons TR. A practical handbook of seawater analysis. Bull Fish Res Board Can 1972;167:49-55.
20. Nedoma J, Vrba J. Specific activity of cell-surface acid phosphatase in different bacter-ioplankton morphotypes in an acidified mountain lake. Environ Microbiol 2006;8:1271-1279.
21. Nedoma J, Štrojsová A, Vrba J, Komárková J, Šimek K. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: Fluorescence quantification and labelling kinetics. Environ Microbiol 2003;5:462-472.
22. Dyhrman S, Ruttemberg KC. Presence and regulation of alkaline phosphatase activity in eukaryotic phytoplankton from the coastal ocean: Implications for dissolved organic phosphorus remineralization. LimnolOceanogr2006;51:1381-1390.
23. Van Wambeke F, Nedoma J, Duhamel S, Lebaron P. Alkaline phosphatase activity studied with ELF97 phosphate: Success and limits in P-limited Mediterranean Sea. Aquat Microb Ecol 2008;52:245-251.
24. Huang Z, You W, Haugland RP, Paragas VB, Olson NA, Haugland RP. A novel fluoro-genic substrate for detecting alkaline phosphatase activity in situ. J Histo chem Cyto-chem 1993;41:313-317.
25. Pernthaler A, Pernthaler J, Amann R. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria. Appl Environ Microbiol 2002;68:3094-3101.