Analysis of recombinant human erythropoietin and novel erythropoiesis stimulating protein digests by immunoaffinity capillary electrophoresis-mass spectrometry

Journal of Chromatography A

Volumn 1216, Issue 12, 2009, Pages 2574-2582

  Giménez, Estela ^a   Benavente, Fernando ^a   de Bolós, Carme ^b   Nicolás, Ernesto ^a   Barbosa, José ^a   Sanz Nebot, Victoria ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b HOSPITAL DEL MAR   (Spain)

Author keywords

Digestion; EPO; IA CE MS; NESP; Peptide marker

Indexed keywords

AMINO ACID SEQUENCES; BIOLOGICAL FLUIDS; CARBOHYDRATE CONTENTS; DIGESTION; EPO; HUMAN ERYTHROPOIETINS; IA-CE-MS; IMMUNOAFFINITY CAPILLARY ELECTROPHORESIS; LIMITS OF DETECTIONS; NESP; PNGASE F; POLYCLONAL; PROTEIN DIGESTS; RECOMBINANT HUMAN ERYTHROPOIETIN (RHEPO); SEPARATION CAPILLARIES; SEPHAROSE 4B; SOLID SUPPORTS; STATIONARY PHASE; SYNTHETIC PEPTIDES;

AMINATION; AMINO ACIDS; ANTIBODIES; CAPILLARY ELECTROPHORESIS; CARBOHYDRATES; CHROMATOGRAPHIC ANALYSIS; ELECTROCHEMISTRY; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; MASS SPECTROMETRY; ORGANIC ACIDS; SPECTRUM ANALYSIS;

AMINES;

NOVEL ERYTHROPOIESIS STIMULATING PROTEIN; RECOMBINANT ERYTHROPOIETIN; SYNTHETIC PEPTIDE; TRYPSIN;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ARTICLE; CAPILLARY ELECTROPHORESIS; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; MASS SPECTROMETRY; PRIORITY JOURNAL;

BIOLOGICAL MARKERS; ELECTROPHORESIS, CAPILLARY; ERYTHROPOIETIN, RECOMBINANT; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOASSAY; MASS SPECTROMETRY; PEPTIDE FRAGMENTS; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TRYPSIN;

EID: 60649112093     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.01.057     Document Type: Article

References (31)

1. Krantz S.B. Blood 77 (1991) 419
2. Fisher J.W. Exp. Biol. Med. 228 (2003) 1
3. Lasne F., and Ceaurriz J. Nature 405 (2000) 635
4. Segura J., Pascual J.A., and Gutiérrez-Gallego R. Anal. Bioanal. Chem. 388 (2007) 1521
5. Egrie J.C., Dwyer E., Browne J.K., Hitz A., and Lykos M.A. Exp. Hematol. 31 (2003) 290
6. Ainsworth S. Chem. Eng. News 85 49 (2007) 13
7. Macdougall I.C. Curr. Hematol. Rep. 4 (2005) 436
8. Lasne F., Martin L., Crepin N., and Ceaurriz J. Anal. Biochem. 311 (2002) 119
9. Neusüβ C., Demelbauer U.M., and Pelzing M. Electrophoresis 26 (2005) 1442
10. Balaguer E., and Neusüβ C. Anal. Chem. 78 (2006) 5384
11. Giménez E., Benavente F., Barbosa J., and Sanz-Nebot V. Electrophoresis 29 (2008) 2161
12. Guan F., Uboh C.E., Soma L.R., and Birks E. Anal. Chem. 79 (2007) 4627
13. Stübiger G., Marchetti M., Nagago M., Grimm R., Gmeiner G., Reichel C., and Allmaier G. J. Sep. Sci. 28 (2005) 1764
14. Llop E., Gutiérrez-Gallego R., Belalcazar V., Gerwig G.J., Kamerling J.P., Segura J., and Pascual J.A. Proteomics 7 (2007) 4278
15. Stroink T., Paarlberg E., Waterval J.C.M., Bult A., and Underberg W.J.M. Electrophoresis 22 (2001) 2374
16. Benavente F., Vescina M.C., Hernández E., Sanz-Nebot V., Barbosa J., and Guzman N.A. J. Chromatogr. A 1140 (2007) 205
17. Guzman N.A., and Phillips T.M. Anal. Chem. 77 (2005) 61A
18. Puig P., Borrull F., Calull M., and Aguilar C. Trends Anal. Chem. 26 (2007) 664
19. Tempels F.W.A., Underberg W.J.M., Somsen G.W., and de Jong G.J. Electrophoresis 29 (2008) 108
20. Benavente F., Hernandez E., Guzman N.A., Sanz-Nebot V., and Barbosa J. Anal. Bioanal. Chem. 387 (2007) 2633
21. Guzman N.A. Electrophoresis 24 (2003) 3718
22. Giménez E., de Bolós C., Belalcazar V., Andreu D., Borrás E., De la Torre B.G., Barbosa J., Segura J., and Pascual J.A. Anal. Bioanal. Chem. 388 (2007) 1531
23. Sanz-Nebot V., Benavente F., Balaguer E., and Barbosa J. Electrophoresis 24 (2003) 883
24. Hernández E., Benavente F., Sanz-Nebot V., and Barbosa J. Electrophoresis 28 (2007) 3957
25. Fuller S.A., Takahashi M., and Hurrell J.G.R. Purification of Monoclonal Antibodies. Curr. Protoc. Mol. Biol. (2001), John Wiley & Sons Chapter 11, Unit 11.11
26. Heiger D. High Performance Capillary Electrophoresis-An Introduction (2000), Agilent Technologies, Waldbronn
27. Simo C., Herrero M., Neusüß C., Pelzing M., Kenndler E., Barbas C., and Cifuentes A. Electrophoresis 26 (2005) 2674
28. Catai J.R., Sastre Toraño J., de Jong G.J., and Somsen G.W. Analyst 132 (2007) 75
29. Guzman N.A. J. Chromatogr. B 749 (2000) 197
30. Lin C.H., and Kaneta T. Electrophoresis 25 (2004) 4058
31. Compute pI/Mw tool. ExPASy (Expert Protein Analysis System) proteomics.server. Swiss Institute of Bioinformatics, Geneva, 1996.