Mesenchymal stem cell-seeded collagen matrices for bone repair: Effects of cyclic tensile strain, cell density, and media conditions on matrix contraction in vitro

Journal of Biomedical Materials Research - Part A

Volumn 88, Issue 3, 2009, Pages 778-786

  Sumanasinghe, Ruwan D ^a,b   Osborne, Jason A ^b   Loboa, Elizabeth G ^a  

^a USA and North Carolina State University   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Bone tissue engineering; Collagen contraction; Mechanical stimulation; Mesenchymal stem cells; Osteogenic differentiation

Indexed keywords

ARGININE-GLYCINE-ASPARTIC ACIDS; BONE CONSTRUCTS; BONE REPAIRS; BONE TISSUE ENGINEERING; CELL DENSITIES; COLLAGEN CONSTRUCTS; COLLAGEN GELS; COLLAGEN MATRICES; CULTURE CONDITIONS; CYCLIC TENSILE STRAINS; DEXAMETHASONE; EXTRACELLULAR MATRIX PROTEINS; GROWTH MEDIAS; HUMAN MESENCHYMAL STEM CELL (HMSCS); IN-VITRO; MATRIXES; MECHANICAL STIMULATION; MESENCHYMAL STEM CELLS; MRNA EXPRESSIONS; OSTEOGENESIS; OSTEOGENIC; OSTEOGENIC DIFFERENTIATION; SCAFFOLD MATERIALS; SEEDING DENSITIES; THREE-DIMENSIONAL (3D); TYPE I COLLAGENS;

ADHESION; AMINO ACIDS; BONE; CELL ADHESION; CELL CULTURE; CELLS; COLLAGEN; FLOW MEASUREMENT; GELATION; GELS; ORGANIC ACIDS; SHRINKAGE; THREE DIMENSIONAL; TISSUE ENGINEERING;

TENSILE STRAIN;

COLLAGEN; COLLAGEN GEL; DEXAMETHASONE;

ADULT; ARTICLE; BONE REMODELING; BONE TISSUE; CELL DENSITY; CELL DIFFERENTIATION; CELL GROWTH; CELL VIABILITY; CULTURE MEDIUM; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; MALE; MESENCHYMAL STEM CELL; OSTEOBLAST; TISSUE ENGINEERING;

ADULT; BONE AND BONES; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SURVIVAL; COLLAGEN; CULTURE MEDIA, CONDITIONED; HUMANS; MALE; MESENCHYMAL STEM CELLS; TENSILE STRENGTH;

EID: 60549108910     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.31913     Document Type: Article

References (32)

1. Bruder SP, Fink DJ, Caplan AI. Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 1994;56:283-294.
2. Caplan AI, Bruder SP. Mesenchymal stem cells: Building blocks for molecular medicine in the 21st century. Trends Mol Med 2001;7:259-264.
3. Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997;64:295-312.
4. Halvorsen YD, Franklin D, Bond AL, Hitt DC, Auchter C, Boskey AL, Paschalis EP, Wilkison WO, Gimble JM. Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. Tissue Eng 2001; 7:729-741.
5. Yoshimoto H, Shin YM, Terai H, Vacanti JP. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials 2003;24:2077-2082.
6. Grayson WL, Ma T, Bunnell B. Human mesenchymal stem cells tissue development in 3D PET matrices. Biotechnol Prog 2004;20:905-912.
7. Meinel L, Karageorgiou V, Hofmann S, Fajardo R, Snyder B, Li C, Zichner L, Langer R, Vunjak-Novakovic G, Kaplan DL. Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J Biomed Mater Res A 2004; 71:25-34.
8. Takahashi Y, Tabata Y. Effect of the fiber diameter and porosity of non-woven PET fabrics on the osteogenic differentiation of mesenchymal stem cells. J Biomater Sci Polym Ed 2004; 15:41-57.
9. Li WJ, Tuli R, Huang X, Laquerriere P, Tuan RS. Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials 2005;26:5158-5166.
10. Ren T, Ren J, Jia X, Pan K. The bone formation in vitro and mandibular defect repair using PLGA porous scaffolds. J Biomed Mater Res A 2005;74:562-569.
11. Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharm 2001;221:1-22.
12. Godbey WT, Atala A. In vitro systems for tissue engineering. Ann NY Acad Sci 2002;961:10-26.
13. Awad HA, Butler DL, Harris MT, Ibrahim RE, Wu Y, Young RG, Kadiyala S, Boivin GP. In vitro characterization of mesenchymal stem cell-seeded collagen scaffolds for tendon repair: Effects of initial seeding density on contraction kinetics. J Biomed Mater Res 2000;51:233-240.
14. Bruder SP, Jaiswal N, Ricalton NS, Mosca JD, Kraus KH, Kadiyala S. Mesenchymal stem cells in osteobiology and applied bone regeneration. Clin Orthop Relat Res 1998(355 Suppl):S247-S256.
15. Niemeyer P, Krause U, Fellenberg J, Kasten P, Seckinger A, Ho AD, Simank HG. Evaluation of mineralized collagen and α-tricalcium phosphate as scaffolds for tissue engineering of bone using human mesenchymal stem cells. Cells Tissues Organs 2004;177:68-78.
16. Bonassar LJ, Vacanti CA. Tissue engineering: The first decade and beyond. J Cell Biochem 1998;30-31:297-303.
17. Langer R. Tissue engineering. Mol Ther 2000;1:12-15.
18. Loboa EG, Fang TD, Warren SM, Lindsey DP, Fong KD, Longaker MT, Carter DR. Mechanobiology of mandibular distraction osteogenesis: Experimental analyses with a rat model. Bone 2004;34:336-343.
19. Loboa EG, Fang TD, Parker DW, Warren SM, Fong KD, Longaker MT, Carter DR. Mechanobiology of mandibular distraction osteogenesis: Finite element analyses with a rat model. J Orthop Res 2005;23:663-670.
20. Sumanasinghe RD, Bernacki S, Loboa EG. Effects of mechanical stimulation on osteogenic differentiation of mesenchymal stem cells in 3D matrices. In: Conference Proceedings Baltimore, Maryland, Biomedical Engineering Society; 2005.
21. Sumanasinghe RD, Bernacki SH, Loboa EG. Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: Effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression. Tissue Eng 2006;12:3459-3465.
22. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA 1979;76:1274-1278.
23. Liu XD, Skold M, Umino T, Zhu YK, Romberger DJ, Spurzem JR, Rennard SI. Endothelial cell-mediated type I collagen gel contraction is regulated by hemin. J Lab Clin Med 2000;136: 100-109.
24. Galois L, Hutasse S, Cortial D, Rousseau CF, Grossin L, Ron-ziere MC, Herbage D, Freyria AM. Bovine chondrocyte behaviour in three-dimensional type I collagen gel in terms of gel contraction, proliferation and gene expression. Biomaterials 2006;27:79-90.
25. Jagodzinski M, Drescher M, Zeichen J, Hankemeier S, Krettek C, Bosch U, van Griensven M. Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. Eur Cell Mater 2004;7:35-41; discussion 41.
26. Simmons CA, Matlis S, Thornton AJ, Chen S, Wang CY, Mooney DJ. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. J Bio-mech 2003;36:1087-1096.
27. Garvin J, Qi J, Maloney M, Banes AJ. Novel system for engineering bioartificial tendons and application of mechanical load. Tissue Eng 2003;9:967-979.
28. Qi J, Chi L, Faber J, Koller B, Banes AJ. ATP reduces gel compaction in osteoblast-populated collagen gels. J App Physiol 2007;102:1152-1160.
29. Nho RS, Xia H, Diebold D, Kahm J, Kleidon J, White E, Henke CA. PTEN regulates firboblast elimination during collagen contraction. J Biol Chem 2006;281:33291-33301.
30. Bowman NN, Donahue HJ, Ehrlich HP. Gap junctional intercellular communication contributes to the contraction of rat osteoblast populated collagen lattices. J Bone Miner Res 1998;13:1700-1706.
31. Harris AK, Wild P, Stopak D. Silicone rubber substrata: A new wrinkle in the study of cell locomotion. Science 1980; 208: 177-179.
32. Nishiyama T, Tominaga N, Nakajima K, Hayashi T. Quantitative evaluation of the factors affecting the process of fibroblast-mediated collagen gel contraction by separating the process into three phases. Coll Relat Res 1988;8:259-273.