A simple and loss-free method to remove TRIzol contaminations from minute RNA samples

Analytical Biochemistry

Volumn 387, Issue 1, 2009, Pages 136-138

  Krebs, Stefan ^a   Fischaleck, Marlis ^a   Blum, Helmut ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLOW CYTOMETRY;

1-BUTANOL; FLUORESCENCE ACTIVATED CELL SORTING; HIGH QUALITY; QUALITY PARAMETERS; SAMPLE SIZES; ULTRAVIOLET SPECTRUM;

RNA;

BUTANOL; PHENOL DERIVATIVE; RNA 18S; RNA 28S; TRIZOL; UNCLASSIFIED DRUG; GUANIDINE DERIVATIVE; RNA;

ARTICLE; CONTAMINATION; FLUORESCENCE ACTIVATED CELL SORTING; PRIORITY JOURNAL; RNA ISOLATION; RNA PURIFICATION; ULTRAVIOLET RADIATION; FLOW CYTOMETRY; ISOLATION AND PURIFICATION; PROCEDURES;

1-BUTANOL; FLOW CYTOMETRY; GUANIDINES; PHENOLS; RNA;

EID: 60549104984     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.020     Document Type: Article

References (3)

1. Chomczynski P., and Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159
2. Chomczynski P., and Sacchi N. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat. Protoc. 1 (2006) 581-585
3. Stafford D.W., and Bieber D. Concentration of DNA solutions by extraction with 2-butanol. Biochim. Biophys. Acta 378 (1975) 18-21