Methods of preparation and analysis of intracellular reverse transcription complexes

Methods in Molecular Biology

Volumn 485, Issue , 2009, Pages 107-119

  Fassati, Ariberto ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

HIV 1; Linear gradient; Purification; Reverse transcription assay; Reverse transcription complex

Indexed keywords

VIRUS DNA; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; GENETICS; HELA CELL; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; ISOLATION AND PURIFICATION; MACROMOLECULE; METABOLISM; PHYSIOLOGY; REVERSE TRANSCRIPTION;

DNA, VIRAL; HELA CELLS; HIV-1; HUMANS; MACROMOLECULAR SUBSTANCES; REVERSE TRANSCRIPTION; RNA, VIRAL; VIRAL PROTEINS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 60349128954     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-170-3_8     Document Type: Article

References (11)

1. Telesnitsky, A., Goff, S. P. (1997) Reverse transcriptase and the generation of retroviral DNA in Retroviruses (Coffin J.M, Hughes S. H., Varmus H.E. eds.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. McDonald, D., Vodicka, M. A., Lucero, G., et al. (2002) Visualization of the intracellular behavior of HIV in living cells. J Cell Biol 159, 441-452.
3. Nermut, M. V., Fassati, A. (2003) Structural analyses of purified human immunodeficiency virus type 1 intracellular reverse transcription complexes. J Virol 77, 8196-8206.
4. Fassati, A., Goff, S. P. (1999) Characterization of intracellular reverse transcription complexes of Moloney murine leukemia virus. J Virol 73, 8919-8925.
5. Fassati, A., Goff, S. P. (2001) Characterization of intracellular reverse transcription complexes of human immunodeficiency type-1. J Virol 75, 3626-3635.
6. Carr, J. M., Davis, A. J., Coolen, C., et al. (2006) Vif-deficient HIV reverse transcription complexes (RTCs) are subject to structural changes and mutation of RTC-associated reverse transcription products. Virology 351, 80-91.
7. Stremlau, M., Perron, M., Lee, M., et al. (2006) Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5alpha restriction factor. Proc Natl Acad Sci USA 103, 5514-5519.
8. Fassati, A., Gorlich, D., Harrison, I., et al. (2003) Nuclear import of HIV-1 intracellular reverse transcription complexes is mediated by importin 7. EMBO J 22, 3675-3685.
9. Zaitseva, L., Mayers, R., Fassati, A. (2006) tRNAs promote nuclear import of HIV-1 intracellular reverse transcription complexes. PloS Biol 4, e332.
10. Naldini, L., Blomer, U., Gallay, P., et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267.
11. Demiason, C., Parsley, K., Brouns, G., et al. (2002) High-level transduction and gene expression in hematopoietic repopulating cells using a HIV-1 based lentiviral vector containing an internal spleen focus forming virus promoter. Hum Gene Ther 13, 803-813.