Selection of recombinant antibodies by eukaryotic ribosome display

Methods in Molecular Biology

Volumn 484, Issue , 2008, Pages 193-205

  He, Mingyue ^a   Taussig, Michael J ^a  

^a BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

Library; Ribosome complex; Selection; Single chain antibody

Indexed keywords

ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; EUKARYOTIC CELL; GENETICS; HUMAN; METHODOLOGY; POLYMERASE CHAIN REACTION; RABBIT; RIBOSOME;

ANIMALS; ANTIBODIES; EUKARYOTIC CELLS; HUMANS; POLYMERASE CHAIN REACTION; RABBITS; RECOMBINANT PROTEINS; RIBOSOMES;

EUKARYOTA; ORYCTOLAGUS CUNICULUS; PROKARYOTA;

EID: 60349089074     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-398-1_13     Document Type: Article

References (8)

1. van Dijk, M. A. and van de Winkel, J. G. (2001) Human antibodies as next generation therapeutics. Curr. Opin. Chem. Biol. 5, 368-374
2. Taussig, M. J., Stoevesandt, O., Borrebaeck, C. A. K., Bradbury, A. R., Cahill, D., et al. (2007) Proteome binders: planning a European resource of affinity reagents for analysis of the human proteome. Nature Methods 4, 13-17.
3. Winter, G., Griffiths, A. D., Hawkins, R. E., and Hoogenboom, H. R. (1994) Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.
4. He, M. and Taussig, M. (2007) Eukaryotic ribosome display with in situ DNA recovery Nature Methods 4, 281-288.
5. He, M. and Taussig, M. J. (2002) Ribosome display: cell-free protein display technology. Briefings Funct Genomics Proteomics 1, 204-212.
6. Zahnd, C., Amstutz, P., and Pluckthun, A. (2007) Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat. Methods 4, 269-279.
7. He, M, Cooley, N., Jackson, A., and Taussig, M. (2004) Production of human single-chain antibodies by ribosome display. In: Methods in Molecular Biology 248: Antibody Engineering Protocols, 2nd ed. (Lo, B., ed.), pp. 177-189. Humana Press, Totowa, NJ.
8. He, M. and Taussig, M. J. (2005) Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system. J. Immunol. Methods 297, 73-82.