Mg2+-free buffer elevates transformation efficiency of Vibrio parahaemolyticus by electroporation

Letters in Applied Microbiology

Volumn 48, Issue 3, 2009, Pages 349-354

  Wang, H ^b   Griffiths, M W ^a,b  

^a UNIVERSITY OF GUELPH   (Canada)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

DNase; Electroporation; Nuclease; Transformation efficiency; Vibrio parahaemolyticus

Indexed keywords

CELLS; CHLORINE COMPOUNDS; CYTOLOGY; EFFICIENCY; SUGAR (SUCROSE);

CONDITION; DNASE; ELECTROPORATION; EXTRACELLULAR; NUCLEASE; SIMPLE++; TRANSFORMANTS; TRANSFORMATION EFFICIENCY; VIBRIO PARAHAEMOLYTICUS; VIBRIO SPP;

MAGNESIUM COMPOUNDS;

BUFFER; DNA; MAGNESIUM; MAGNESIUM CHLORIDE; NUCLEASE; SUCROSE; BACTERIAL DNA; DEOXYRIBONUCLEASE;

CELLS; CONCENTRATION (PROCESS); DNA; PATHOGENS;

BACTERIUM; BIOTRANSFORMATION; BUFFERING; CONCENTRATION (COMPOSITION); MAGNESIUM; MICROBIAL ACTIVITY;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL VIRULENCE; BACTERIUM TRANSFORMATION; CELL MEMBRANE; ELECTROPORATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENETIC ANALYSIS; NONHUMAN; ORGANISM; PATHOGENICITY; VIBRIO PARAHAEMOLYTICUS; ANIMAL; BIVALVE; CULTURE MEDIUM; ENZYMOLOGY; EVALUATION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PH;

VIBRIO; VIBRIO PARAHAEMOLYTICUS;

ANIMALS; BIVALVIA; BUFFERS; CULTURE MEDIA; DEOXYRIBONUCLEASES; DNA, BACTERIAL; ELECTROPORATION; HYDROGEN-ION CONCENTRATION; MAGNESIUM; SUCROSE; TRANSFORMATION, GENETIC; VIBRIO PARAHAEMOLYTICUS;

EID: 60349085324     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2008.02531.x     Document Type: Article

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