Discrimination of a transformation phenotype in Syrian golden hamster embryo (SHE) cells using ATR-FTIR spectroscopy

Toxicology

Volumn 258, Issue 1, 2009, Pages 33-38

  Walsh, Michael J ^a   Bruce, Shannon W ^b   Pant, Kamala ^b   Carmichael, Paul L ^c   Scott, Andrew D ^c   Martin, Francis L ^a  

^a LANCASTER UNIVERSITY   (United Kingdom)

^b BioReliance Corporation   (United States)

^c UNILEVER RESEARCH   (United Kingdom)

Author keywords

ATR FTIR spectroscopy; Benzo a pyrene; Linear discriminant analysis; Morphological transformation; Principal component analysis; Syrian hamster embryo cells

Indexed keywords

AMIDE; BENZO[A]PYRENE; CARCINOGEN; PHOSPHATE;

ANIMAL CELL; ARTICLE; ATTENUATION; BIOPHYSICS; CELL CULTURE; CELL STRUCTURE; CONTROLLED STUDY; CRYSTAL; DISCRIMINANT ANALYSIS; EMBRYO; INFRARED SPECTROSCOPY; MICROSCOPY; MULTIVARIATE ANALYSIS; NONHUMAN; PH; PHENOTYPE; PRINCIPAL COMPONENT ANALYSIS; PRIORITY JOURNAL; RETROSPECTIVE STUDY; SCORING SYSTEM; SPECTROMETER; SPECTRUM; STAINING; SYRIAN HAMSTER; VIBRATION;

ANIMALS; BENZO(A)PYRENE; CARCINOGENS; CELL COUNT; CELL LINE; CELL TRANSFORMATION, NEOPLASTIC; CRICETINAE; EMBRYO, MAMMALIAN; MESOCRICETUS; PHENOTYPE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

MESOCRICETUS AURATUS;

EID: 60249097808     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2009.01.003     Document Type: Article

References (20)

1. Barber J.L., Walsh M.J., Hewitt R., Jones K.C., and Martin F.L. Low-dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells. Mutagenesis 21 (2006) 351-360
2. Bentley A.J., Nakamura T., Hammiche A., Pollock H.M., Martin F.L., Kinoshita S., and Fullwood N.J. Characterization of human corneal stem cells by synchrotron infrared micro-spectroscopy. Mol. Vis. 22 (2007) 237-242
3. Engelhardt G., Schwind K.-R., and Muβler B. The testing of chemicals in the Syrian hamster embryo (SHE) cell transformation assay for assessment of carcinogenic potential. Toxicol. In Vitro 18 (2004) 213-218
4. German M.J., Hammiche A., Ragavan N., Tobin M.J., Cooper L.J., Matanhelia S.S., Hindley A.C., Nicholson C.M., Fullwood N.J., Pollock H.M., and Martin F.L. Infrared spectroscopy with multivariate analysis potentially facilitates the segregation of different types of prostate cell. Biophys. J. 90 (2006) 3783-3795
5. German M.J., Pollock H.M., Zhao B., Tobin M.J., Hammiche A., Bentley A., Cooper L.J., Martin F.L., and Fullwood N.J. Characterization of putative stem cell populations in the cornea using synchrotron infrared microspectroscopy. Invest. Ophthalmol. Vis. Sci. 47 (2006) 2417-2421
6. Goormaghtigh E., Ruysschaert J.M., and Raussens V. Evaluation of the information content in infrared spectra for protein secondary structure determination. Biophys. J. 90 (2006) 2946-2957
7. Hammiche A., German M.J., Hewitt R., Pollock H.M., and Martin F.L. Monitoring cell cycle distributions in MCF-7 cells using near-field photothermal microspectroscopy. Biophys. J. 88 (2005) 3699-3706
8. Isfort R.J., Cody D.B., Doerson C., Kerckaert G.A., and LeBoeuf R.A. Alterations in cellular differentiation, mitogenesis, cytoskeleton and growth characteristics during Syrian hamster embryo cell multistep in vitro transformation. Int. J. Cancer 59 (1994) 114-125
9. Kerckaert G.A., Isfort R.J., Carr G.J., Aardema M.J., and LeBoeuf R.A. A comprehensive protocol for conducting the Syrian hamster embryo cell transformation assay at pH 6.70. Mutat. Res. 356 (1996) 65-84
10. LeBoeuf R.A., and Kerckaert G. Enhanced morphological transformation of early passage Syrian hamster embryo cells cultured in medium with a reduced bicarbonate concentration and pH. Carcinogenesis 8 (1987) 680-697
11. LeBoeuf R.A., Kerckaert G.A., Aardema M.J., and Gibson D.P. Multistage neoplastic transformation of Syrian hamster embryo cells cultured at pH 6.70. Cancer Res. 50 (1990) 3722-3729
12. LeBoeuf R.A., Kerckaert G.A., Aardema M.J., Gibson D.P., Brauninger R., and Isfort R.J. The pH 6.7 Syrian hamster embryo cell transformation assay for assessing the carcinogenic potential of chemicals. Mutat. Res. 356 (1990) 85-127
13. LeBoeuf R.A., Kerckaert G.A., Aardema M.J., Gibson D.P., Brauninger R., and Isfort R.J. The pH 6.7 Syrian hamster embryo cell transformation assay for assessing the carcinogenic potential of chemicals. Mutat. Res. 356 (1996) 85-127
14. Maire M.A., Rast C., Landkocz Y., and Vasseur P. 2,4-Dichlorophenoxyacetic acid: effects on Syrian hamster embryo (SHE) cell transformation, c-Myc expression. DNA damage and apoptosis. Mutat. Res. 631 (2007) 124-136
15. Mantsch H.H., and Chapman D. Infrared Spectroscopy of Biomolecules (1996), John Wiley & Sons, New York
16. Martin F.L., German M.J., Wit E., Fearn T., Ragavan N., and Pollock H.M. Identifying variables responsible for clustering in discriminant analysis of data from infrared microspectroscopy of a biological sample. J. Comput. Biol. 14 (2007) 1176-1184
17. Mauthe R.J., Gibson D.P., Bunch R.T., and Custer L. The Syrian hamster embryo (SHE) cell transformation assay: review of the methods and results. Toxicol. Pathol. 29 Suppl. l (2001) 138-146
18. Walsh M.J., German M.J., Singh M., Pollock H.M., Hammiche A., Kyrgiou M., Stringfellow H.F., Paraskevaidis E., Martin-Hirsch P.L., and Martin F.L. IR microspectroscopy: potential applications in cervical cancer screening. Cancer Lett. 246 (2007) 1-11
19. Walsh M.J., Singh M.N., Pollock H.M., Cooper L.J., German M.J., Stringfellow H.F., Fullwood N.J., Paraskevaidis E., Martin-Hirsch P.L., and Martin F.L. ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology. Biochem. Biophys. Res. Commun. 352 (2007) 213-219
20. - modifications as a marker of the putative stem cell region of human intestinal crypts. Stem Cells 26 (2008) 108-118 10.1634/stemcells.2007-0196