Nested PCR-RFLP is a high-speed method to detect fungicide-resistant Botrytis cinerea at an early growth stage of grapes

Pest Management Science

Volumn 65, Issue 2, 2009, Pages 197-204

  Saito, Seiya ^a   Suzuki, Shunji ^a   Takayanagi, Tsutomu ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Botrytis cinerea; Fungicide resistance; Grapevine; Nested PCR RFLP

Indexed keywords

BENZIMIDAZOLE; BENZIMIDAZOLE DERIVATIVE; CARBAMIC ACID DERIVATIVE; FUNGICIDE;

DETECTION METHOD; FUNGICIDE; FUNGUS; PESTICIDE RESISTANCE; POLYMERASE CHAIN REACTION; VINE;

ANTIFUNGAL RESISTANCE; ARTICLE; BOTRYTIS; DRUG EFFECT; EVALUATION; GENETICS; GRAPE; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PLANT DISEASE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

BENZIMIDAZOLES; BOTRYTIS; DRUG RESISTANCE, FUNGAL; FUNGICIDES, INDUSTRIAL; PHENYLCARBAMATES; PLANT DISEASES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; VITIS;

BOTRYOTINIA FUCKELIANA; BOTRYTIS; FUNGI; VITACEAE; VITIS;

EID: 60249094732     PISSN: 1526498X     EISSN: 15264998     Source Type: Journal    
DOI: 10.1002/ps.1668     Document Type: Article

References (23)

1. Elad Y, Williamson B, Tudzynski P and Delen N, Botrytis: Biology, Pathology and Control. Kluwer Academic Publishers, Dordrecht, The Netherlands p. 428 (2004).
2. Keller M, Viret O and Cole FM, Botrytis cinerea infection in grape flowers: defense reaction, latency, and disease expression. Phytopathology 93:316-322 (2003).
3. Elmer PAG and Reglinski T, Biosuppression of Botrytis cinerea in grapes. Plant Pathol 55:155-177 (2006).
4. Kalamarakis AE, Petsikos-Panagiotarou N, Mavroidis B and Ziogas BN, Activity of fluazinam against strains of Botrytis cinerea resistant to benzimidazoles and/or dicarboximides and to a benzimidazole-phenylcarbamate mixture. J Phytopathol 148:449-455 (2000).
5. Leroux P, Fritz R, Debieu D, Albertini C, Lanen C, Bach J, et al, Mechanisms of resistance to fungicides in field strains of Botrytis cinerea. Pest Manag Sci 58:876-888 (2002).
6. O'Brien RG and Glass RJ, The appearance of dicarboximide resistance in Botrytis cinerea in Queensland. Aust Plant Pathol 15:24-25 (1986).
7. Butters JA and Hollomon DW, Resistance to benzimidazole can be caused by changes in β-tubulin isoforms. Pestic Sci 55:486-503 (1999).
8. Luck JE, Gillings MR and Steel CC, Amplification and cloning of a β-tubulin gene fragment from strains of Botrytis cinerea resistant and sensitive to benzimidazole fungicides. NZ J Crop Hortic Sci 22:173-179 (1994).
9. Nakazawa Y and Yamada M, Chemical control of grey mould in Japan. A history of combating resistance. Agrochem Jpn 71:2-6 (1997).
10. Paplomatas EJ, Pappas AC and Antoniadis D, A relationship among fungicide-resistant phenotypes of Botrytis cinerea based on RAPD analysis. J Phytopathol 152:503-508 (2004).
11. Yourman LF and Jeffers SN, Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Dis 83:569-575 (1999).
12. Yourman LF, Jeffers SN and Dean RA, Genetic analysis of isolates of Botrytis cinerea sensitive and resistant to benzimidazole and dicarboximide fungicides. Phytopathol 90:851-859 (2006).
13. Cui W, Beever RE, Parkes SL, Weeds PL and Templeton MD, An osmosensing histidine kinase mediates dicarboximide fungicide resistance in Botryotinia fuckeliana (Botrytis cinerea). Fungal Genet Biol 36:187-198 (2002).
14. Oshima M, Fujimura M, Banno S, Hashimoto C, Motoyama T, Ichiishi A, et al, A point mutation in the two-component histidine kinase BcOS1 gene confers dicarboximide resistance in field isolates of Botrytis cinerea. Phytopathology 92:75-80 (2002).
15. Murray MG and Thompson WF, Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-4325 (1980).
16. Yarden O and Katan T, Mutation leading to substitutions at amino acids 198 and 200 of beta-tubulin that correlated with benomyl-resistance phenotypes of field strains of Botrytis cinerea. Phytopathology 83:1478-1483 (1993).
17. Oshima M, Banno S, Okada K, Takeuchi T, Kimura M, Ichiishi A, et al, Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea. J Gen Plant Pathol 72:65-73 (2006).
18. Coombe BG, Adoption of a system for identifying grapevine growth stages. Aust J Grape and Wine Res 1:100-110 (1995).
19. Cañas-Guiérrez GP, Patiño LF, Rodríguez-Arango E and Arango R, Molecular characterization of benomyl-resistant isolates of Mycosphaerella fijensis, collected in Colombia. J Phytopathol 154:403-409 (2006).
20. Ishii H, Fraaije BA, Sugiyama T, Noguchi K, Nishimura K, Takeda T, et al, Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91:1166-1171 (2001).
21. Ma Z, Yoshimura MA, Holts BA and Michailides TJ, Characterization and PCR-based detection of benzimidazole-resistant isolates of Monilinia laxa in California. Pest Manag Sci 61:449-457 (2005).
22. McKay GJ and Cooke LR, A PCR-based method to characterize and identify benzimidazole resistance in Helminthosporium solani. FEMS Microbiol Lett 152:371-378 (1997).
23. Banno S, Fukumori F, Ichiishi A, Okada K, Uekusa H, Kimura M, et al, Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays. Phytopathology 98:397-404 (2008).