Rapid quantitation of hemoglobin S in sickle cell patients using the variant II turbo analyzer

Annals of Clinical and Laboratory Science

Volumn 39, Issue 1, 2009, Pages 32-37

  Edwards, Henry D ^a   Sabio, Hernan ^a   Shihabi, Zak K ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

Electrophoresis; Fetal hemoglobin; Glycated hemoglobin; HbA1c; Hemoglobin quantification; Hemoglobinopathies; HPLC; Sickle cell disease

Indexed keywords

HEMOGLOBIN A1C; HEMOGLOBIN C; HEMOGLOBIN F; HEMOGLOBIN S;

ANALYZER; ARTICLE; BLOOD ANALYSIS; DIAGNOSTIC ACCURACY; ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SICKLE CELL ANEMIA;

ANEMIA, SICKLE CELL; BLOOD TRANSFUSION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, AGAR GEL; FETAL HEMOGLOBIN; HEMOGLOBIN A, GLYCOSYLATED; HEMOGLOBIN, SICKLE; HUMANS;

EID: 60249088942     PISSN: 00917370     EISSN: 15508080     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Yale SH, Nagib N, Guthrie T. Acute chest syndrome in sickle cell disease. Postgrad Med 2000;107:215-222.
2. Adams RJ, McKie VC, Hsu L, Files B, Vichinsky E, Pegelow C, Abboud M, Gallagher D, Kutlar A, Nichols FT, Bonds DR, Brambilla D. Prevention of a first stroke by transfusions in children with sickle cell anemia and abnormal results on transcranial doppler ultrasono-graphy. NEJM 1998;339:5-11.
3. Pegelow CH, Adams RJ, McKie VC, Abboud M, Berman B, Miller ST, Olivieri N, Vichinsky E, Wang W, Brambilla D. Risk of recurrent stroke in patients with sickle cell disease treated with erythrocyte transfusions. J Pediatrics 1995;126:896-899.
4. Cohen AR, Martin MB, Silber JH, Kim HC, Ohene-Frempong K, Schwartz E. A modified transfusion program for prevention of stroke in sickle cell disease. Blood 1992;79:1657-1661.
5. Vichinsky EP, Haberkern CM, Neumayr L, Earles AN, Black D, Koshy M, Pegelow C, Abboud M, Ohene-Frempong K, Iyer RV. A comparison of conservative and aggressive transfusion regimens in the perioperative management of sickle cell disease. NEJM 1995;333:206-213.
6. Rosse WF, Gallagher D, Kinney TR, Castro O, Dosik H, Moohr J, Wang W, Levy PS. Transfusion and alloimmunization in sickle cell disease. Blood 1990;76: 1431-1437.
7. Kozarski TB, Howanitz PJ, Howanitz JH, Lilic N, Chauhan YS. Blood transfusions leading to apparent hemoglobin C, S, and O-Arab hemoglobinopathies. Arch Pathol Lab Med 2006;130:1830-1833.
8. Yale SH, Nagib N, Guthrie T. Acute chest syndrome in sickle cell disease. Postgrad Med 2000;107:215-222.
9. Kozanoglu I, Boga C, Ozdogu H, Sezgin N, Kizilkilic E, Kural M. Automated red cell exchange procedures in patients with sickle cell disease. Transfus Apher Sci 2007;36:305-312.
10. Lepp CA, Bluestein BI. Hemoglobin electrophoresis at alkaline pH on agarose gels. Clin Chem 1978;24:936-937.
11. Joutovsky A, Hadzi-Nesic J, Nardi MA. HPLC retention time as a diagnostic tool for hemoglobin variants and hemoglobinopathies: A study of 60000 samples in a clinical diagnostic laboratory. Clin Chem 2004;50:1736-1747.
12. Papadea C, Cate JC. Identification of quantification of hemoglobins A, F, S, and C by automated chromato-graphy. Clin Chem 1996;42:57-63.
13. Shihabi ZK. Clinical application ofcapillary electrophoresis. In: Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, (Landers J, Ed), CRC Press, Boca Raton, 2008; pp 785-810.
14. Zak B, Baginski, ES, Epstein E. Associated problems of protein electrophoresis, staining and densitometry. Ann Clin Lab Sci 1978;5:385-395.
15. 2 quantification by capillary zone electrophoresis. Electrophoresis 2000;21:749-752.
16. Shihabi ZK. Effect of sample composition on electro- phoretic migration application to hemoglobin analysis by capillary electrophoresis and agarose electrophoresis. J Chromatogr A 2004;1027:179-184.
17. Mehta SR, Adams RJ. Treatment and prevention of stroke in children with sickle cell disease. Curr Treat Options Neurol 2006;8:503-512.
18. Mehta SR, Aenyi-Annan A, Byrns PJ, Lottenberg R. Opportunities to improve outcomes in sickle cell disease. Am Fam Physician 2006;74:303-312.
19. Higgins TN, Ridley B, Clarke G. Novel method for screening for the presence of haemoglobin S in blood. Transfusion Med 2005;15:493-497.
20. Higgins TN, Ridley B. Tentative identification of hemoglobin variants in the Bio-Rad VARIANT II Hb A1c method. Clin Biochem 2005;38:272-277.
21. Thomas LB, Agosti SJ, Man MA, Mastorides SM. Screening for hemoglobinopathies during routine hemoglobin A1c testing using the Tosoh G7 glycohemoglobin analyzer. Ann Clin Lab Sci 2007,37:251-255.