Identification and quantification of hemoglobins A, F, S, and C by automated chromatography

Clinical Chemistry

Volumn 42, Issue 1, 1996, Pages 57-63

  Papadea, Christine ^a,b   Cate IV, John C ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

cation exchange; chromatography; hemoglobinopathies; intermethod comparison

Indexed keywords

ALKALI; BILIRUBIN; HEMOGLOBIN A; HEMOGLOBIN A2; HEMOGLOBIN C; HEMOGLOBIN F; HEMOGLOBIN S; TRIACYLGLYCEROL;

ADULT; ARTICLE; AUTOANALYZER; BETA THALASSEMIA; CHILD; CHROMATOGRAPHY; CONTROLLED STUDY; DENATURATION; DIAGNOSTIC ACCURACY; ELECTROPHORESIS; HEMOGLOBIN DETERMINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; SICKLE CELL ANEMIA;

EID: 0030068420     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/42.1.57     Document Type: Article

References (14)

1. Bunn HF. Disorders of hemoglobin. In: Isselbacher KJ, Braunwald E, Wilson JD, Martin JB, Fauci AS, Kasper DL, eds. Harrison's principles of internal medicine. New York: McGraw-Hill, 1994: 1734-43.
2. Ou CN, Rognerud CL. Rapid analysis of hemoglobin variants by cation-exchange chromatography. Clin Chem 1993;39:820-4.
3. Papadea C, Eckman JR, Kuehnert RS, Platt AF. Comparison of liquid and dried blood for neonatal hemoglobinopathy screening: laboratory and programmatic issues. Pediatrics 1994;93:427-32.
4. Huisman THJ, Schroeder WA, Brodie AN, Mayson SM, Jakway J. Microchromatography of hemoglobins. III. A simplified procedure for the determination of hemoglobin A2. J Lab Clin Med 1975;86: 700-2.
5. Singer K, Chernoff AI, Singer L. Studies on abnormal hemoglobins. I. Their demonstration in sickle cell anemia and hematologic disorders by means of alkali denaturation. Blood 1951;6:413-27.
6. Wilson JB, Headlee ME, Huisman THJ. A new high performance liquid chromatographic procedure for the separation and quantitation of various hemoglobin variants in adults and newborn babies. J Lab Clin Med 1983;102:174-86.
7. Rogers BB, Wessels RA, Ou CN, Buffone CJ. High performance liquid chromatography in the diagnosis of hemoglobinopathies and thalassemias. Am J Clin Pathol 1985;84:671-4.
8. Tan GB, Aw TC, Dunstan RA, Lee SH. Evaluation of high performance liquid chromatography for routine estimation of hemoglobins A2 and F. J Clin Pathol 1993;46:852-6.
9. Galanello R, Barella S, Gasperini D, Perseu L, Paglietti E, Sollaino C, et al. Evaluation of an automatic HPLC analyzer for thalassemia and hemoglobin variants screening. J Autom Chem 1995;17: 73-6.
10. NCCLS. Tentative guideline EP5-T2. Evaluation of precision performance of clinical chemistry devices, 2nd ed. Villanova, PA: National Committee for Clinical Laboratory Standards, March 1992.
11. Büttner J, Borth R, Boutwell JH, Broughton PMG. International Federation of Clinical Chemistry provisional recommendations on quality control in clinical chemistry: Part I. General principles and terminology. Clin Chem 1976;22:532-40.
12. NCCLS. Proposed guideline. How to define, determine, and utilize reference intervals in the clinical laboratory. Villanova, PA: National Committee for Clinical Laboratory Standards, March 1992.
13. Hempe JM, Craver RD. Quantification of hemoglobin variants by capillary isoelectric focusing. Clin Chem 1994;40:2288-95.
14. Vichinsky EP, Haberkern CM, Neumayr L, Earles AN, Black D, Koshy M, et al. A comparison of conservative and aggressive transfusion regimens in the perioperative management of sickle cell disease. N Engl J Med 1995;333:206-13.