In vitro bactericidal activity of recombinant human β-defensin-3 against pathogenic bacterial strains in human tooth root canal

International Journal of Antimicrobial Agents

Volumn 33, Issue 3, 2009, Pages 237-243

  Song, Wei ^a   Shi, Yong ^a   Xiao, Mingzhen ^a   Lu, Hong ^a   Qu, Tiejun ^a   Li, Ping ^a   Wu, Gang ^a   Tian, Yu ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antimicrobial activity; Human defensin 3; Infected root canals; Pathogenic microbes; Recombinant protein

Indexed keywords

BETA DEFENSIN 3;

ACTINOMYCES NAESLUNDII; ARTICLE; BACTERIAL STRAIN; BACTERICIDAL ACTIVITY; CANDIDA ALBICANS; ENDODONTICS; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FUSOBACTERIUM NUCLEATUM; GENETIC ENGINEERING; HUMAN; HUMAN TISSUE; IN VITRO STUDY; MICROENVIRONMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTOSTREPTOCOCCUS; PREVOTELLA MELANINOGENICA; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS MUTANS; TOOTH INFECTION; TOOTH ROOT CANAL;

ANTI-BACTERIAL AGENTS; BACTERIA; BETA-DEFENSINS; DENTAL PULP CAVITY; FUNGI; HUMANS; MICROBIAL SENSITIVITY TESTS; MICROBIAL VIABILITY; RECOMBINANT PROTEINS;

EID: 60249087859     PISSN: 09248579     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijantimicag.2008.05.022     Document Type: Article

References (35)

1. Sen B.H., Piskin B., and Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Endod Dent Traumatol 11 (1995) 6-9
2. Foschi F., Cavrini F., Montebugnoli L., Stashenko P., Sambri V., and Prati C. Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol 20 (2005) 289-295
3. Nair P.N., Sjögren U., Krey G., Kahnberg K.E., and Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 16 (1990) 580-588
4. Waltimo T.M., Sirén E.K., Torkko H.L., Olsen I., and Haapasalo M.P. Fungi in therapy-resistant apical periodontitis. Int Endod J 30 (1997) 96-101
5. Fouad A.F., Zerella J., Barry J., and Spangberg L.S. Molecular detection of Enterococcus species in root canals of therapy-resistant endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99 (2005) 112-118
6. Sjogren U., Figdor D., Spangberg L., and Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J 24 (1991) 119-125
7. Lewis B. Formaldehyde in dentistry: a review for the millennium. J Clin Pediatr Dent 22 (1998) 167-177
8. Chong B.S., and Pitt Ford T.R. The role of intracanal medication in root canal treatment. Int Endod J 25 (1992) 97-106
9. Foreman P.C., and Barnes I.E. Review of calcium hydroxide. Int Endod J 23 (1990) 283-297
10. Leonardo R.T., Consolaro A., Carlos I.Z., and Leonardo M.R. Evaluation of cell culture cytotoxicity of five root canal sealers. J Endod 26 (2000) 328-330
11. Ganz T. Defensins and host defense. Science 286 (1999) 420-421
12. Ganz T. Defensins and other antimicrobial peptides: a historical perspective and an update. Comb Chem High Throughput Screen 8 (2005) 209-217
13. Harder J., Bartels J., Christophers E., and Schroder J.M. Isolation and characterization of human β-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276 (2001) 5707-5713
14. Maisetta G., Batoni G., Esin S., Florio W., Bottai D., Favilli F., et al. In vitro bactericidal activity of human β-defensin 3 against multidrug-resistant nosocomial strains. Antimicrob Agents Chemother 50 (2006) 806-809
15. Bals R., Wang X., Wu Z., Freeman T., Bafna V., Zasloff M., et al. Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J Clin Invest 102 (1998) 874-880
16. Lu Q., Samaranayake L.P., Darveau R.P., and Jin L. Expression of human beta-defensin-3 in gingival epithelia. J Periodontal Res 40 (2005) 474-481
17. Maisetta G., Batoni G., Esin S., Raco G., Bottai D., Favilli F., et al. Susceptibility of Streptococcus mutans and Actinobacillus actinomycetemcomitans to bactericidal activity of human beta-defensin 3 in biological fluids. Antimicrob Agents Chemother 49 (2005) 1245-1248
18. Ji S., Hyun J., Park E., Lee B.L., Kim K.K., and Choi Y. Susceptibility of various oral bacteria to antimicrobial peptides and to phagocytosis by neutrophils. J Periodontal Res 42 (2007) 410-419
19. Dale B.A., Tao R., Kimball J.R., and Jurevic RJ. Oral antimicrobial peptides and biological control of caries. BMC Oral Health 15 Suppl. (1) (2006) S13
20. Sambrook J., Fritsch E.F., and Maniatis T. Molecular cloning: a laboratory manual. 3rd ed. (2002), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY [in Chinese]
21. Carson K.R., Goodell G.G., and McClanahan S.B. Comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens. J Endod 31 (2005) 471-473
22. Maisetta G., Batoni G., Esin S., Luperini F., Pardini M., Bottai D., et al. Activity of human beta-defensin 3 alone or combined with other antimicrobial agents against oral bacteria. Antimicrob Agents Chemother 47 (2003) 3349-3351
23. Joly S., Maze C., McCray Jr. P.B., and Guthmiller JM. Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. J Clin Microbiol 42 (2004) 1024-1029
24. Hoover D.M., Wu Z., Tucker K., Lu W., and Lubkowski J. Antimicrobial characterization of human β-defensin 3 derivatives. Antimicrob Agents Chemother 47 (2003) 2804-2809
25. Sahly H., Schubert S., Harder J., Rautenberg P., Ullmann U., Schröder J., et al. Burkholderia is highly resistant to human beta-defensin 3. Antimicrob Agents Chemother 47 (2003) 1739-1741
26. Ouhara K., Komatsuzawa H., Yamada S., Shiba H., Fujiwara T., Ohara M., et al. Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, β-defensins and LL37, produced by human epithelial cells. J Antimicrob Chemother 55 (2005) 888-896
27. Schibli D.J., Hunter H.N., Aseyev V., Starner T.D., Wiencek J.M., McCray Jr. P.B., et al. The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus. J Biol Chem 277 (2002) 8279-8289
28. Peschel A., Jack R.W., Otto M., Collins L.V., Staubitz P., Nicholson G., et al. Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine. J Exp Med 7 (2001) 1067-1076
29. Peschel A., Otto M., Jack R.W., Kalbacher H., Jung G., and Götz F. Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides. J Biol Chem 274 (1999) 8405-8410
30. Pallotta R.C., Ribeiro M.S., and de Lima Machado ME. Determination of the minimum inhibitory concentration of four medicaments used as intracanal medication. Aust Endod J 33 (2007) 107-111
31. Oncag O., Cogulu D., Uzel A., and Sorkun K. Efficacy of propolis as an intracanal medicament against Enterococcus faecalis. Gen Dent 54 (2006) 319-322
32. Krithikadatta J., Indira R., and Dorothykalyani A.L. Disinfection of dentinal tubules with 2% chlorhexidine, 2% metronidazole, bioactive glass when compared with calcium hydroxide as intracanal medicaments. J Endod 33 (2007) 1473-1476
33. Böhling A., Hagge S.O., Roes S., Podschun R., Sahly H., Harder J., et al. Lipid-specific membrane activity of human beta-defensin-3. Biochemistry 45 (2006) 5663-5670
34. Batoni G., Maisetta G., Esin S., and Campa M. Human beta-defensin-3: a promising antimicrobial peptide. Mini Rev Med Chem 6 (2006) 1063-1073
35. Klüver E., Schulz-Maronde S., Scheid S., Meyer B., Forssmann W.G., and Adermann K. Structure-activity relation of human beta-defensin 3: influence of disulfide bonds and cysteine substitution on antimicrobial activity and cytotoxicity. Biochemistry 44 (2005) 9804-9816