Functional expression of human HMG-CoA reductase in Saccharomyces cerevisiae: A system to analyse normal and mutated versions of the enzyme in the context of statin treatment

Journal of Applied Microbiology

Volumn 106, Issue 3, 2009, Pages 895-902

  Wysocka Kapcinska, M ^a   Lutyk Nadolska, J ^a   Kiliszek, M ^b   Plochocka, D ^a   Maciag, M ^a   Leszczynska, A ^a   Rytka, J ^a   Burzynska, B ^a  

^a INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

^b MEDICAL UNIVERSITY OF WARSAW   (Poland)

Author keywords

HMG CoA reductase; Statins; Yeast expression system

Indexed keywords

BLOOD; CHOLESTEROL; ENZYME ACTIVITY; GENE EXPRESSION; MAMMALS;

3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE; BLOOD CHOLESTEROL; CHOLESTEROL LEVELS; COENZYME A; EXPRESSION SYSTEM; FUNCTIONAL EXPRESSION; STATINS; WILD TYPES; YEAST EXPRESSION; YEAST EXPRESSION SYSTEM;

YEAST;

ATORVASTATIN; DNA; FLUINDOSTATIN; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; MUTANT PROTEIN; SIMVASTATIN;

ENZYME ACTIVITY; GENE EXPRESSION; INHIBITION; MAMMAL; MUTATION; YEAST;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; CARDIOVASCULAR DISEASE; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; ENZYME STRUCTURE; FUNGAL GENE; FUNGAL STRAIN; GENE EXPRESSION SYSTEM; GENE MUTATION; GENE SEQUENCE; GENETIC ANALYSIS; HUMAN; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; WILD TYPE; YEAST;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 60049095695     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04060.x     Document Type: Article

References (20)

1. Basson, M.E., Thorsness, M., Finer-Moore, J., Stroud, R.M. Rine, J. (1988) Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis. Mol Cell Biol 8, 3797 3808.
2. Chasman, D.I., Posada, D., Subrahmanyan, L., Cook, N.R., Stanton, V.P. Jr. Ridker, P.M. (2004) Pharmacogenetic study of statin therapy and cholesterol reduction. JAMA 291, 2821 2827.
3. Gresser, U. Gathof, B.S. (2004) Atorvastatin: gold standard for prophylaxis of myocardial ischemia and stroke - comparison of the clinical benefit of statins on the basis of randomized controlled endpoint studies. Eur J Med Res 9, 1 17.
4. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. Pease, L.R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51 59.
5. Istvan, E.S. (2001) Bacterial and mammalian HMG-CoA reductases: related enzymes with distinct architectures. Curr Opin Struct Biol 11, 746 751.
6. Istvan, E.S. Deisenhofer, J. (2000) The structure of the catalytic portion of human HMG-CoA reductase. Biochim Biophys Acta 1529, 9 18.
7. Kajinami, K., Takekoshi, N., Brousseau, M.E. Schaefer, E.J. (2004) Pharmacogenetics of HMG-CoA reductase inhibitors: exploring the potential for genotype-based individualization of coronary heart disease management. Atherosclerosis 177, 219 234.
8. Lennernäs, H. Fager, G. (1997) Pharmacodynamics and pharmacokinetics of the HMG-CoA reductase inhibitors. Similarities and differences. Clin Pharmacokinet 32, 403 425.
9. Macreadie, I.G., Johnson, G., Schlosser, T. Macreadie, P.I. (2006) Growth inhibition of Candida species and Aspergillus fumigatus by statins. FEMS Microbiol Lett 262, 9 13.
10. Maitland-van der Zee, A.H. Boerwinkle, E. (2005) Pharmacogenetics of response to statins: where do we stand? Curr Atheroscler Rep 7, 204 208.
11. Mangravite, L.M. Krauss, R.M. (2007) Pharmacogenomics of statin response. Curr Opin Lipidol 18, 409 414.
12. Martin, J.H. Krum, H. (2007) Statins and clinical outcomes in heart failure. Clin Sci (Lond) 113, 119 127.
13. Panda, T. Devi, V.A. (2004) Regulation and degradation of HMGCo-A reductase. Appl Microbiol Biotechnol 66, 143 152.
14. Recto, C.S. II., Acosta, S. Dobs, A. (2000) Comparison of the efficacy and tolerability of simvastatin and atorvastatin in the treatment of hypercholesterolemia. Clin Cardiol 23, 682 688.
15. Rose, M., Winston, F. Hieter, P. (1990) Methods in Yeast Genetics. A Cold Spring Harbor Laboratory Course. New York : Cold Spring Harbor Press.
16. Schaefer, E.J., McNamara, J.R., Tayler, T., Daly, J.A., Gleason, J.L., Seman, L.J., Ferrari, A. Rubenstein, J.J. (2004) Comparisons of effects of statins (atorvastatin, fluvastatin, lovastatin, pravastatin, and simvastatin) on fasting and postprandial lipoproteins in patients with coronary heart disease versus control subjects. Am J Cardiol 93, 31 39.
17. Thompson, J.F., Man, M., Johnson, K.J., Wood, L.S., Lira, M.E., Lloyd, D.B., Banerjee, P., Milos, P.M. et al. (2005) An association study of 43 SNPs in 16 candidate genes with atorvastatin respons. Pharmacogenomics J 5, 352 358.
18. Voth, W.P., Jiang, Y.W. Stillman, D.J. (2003) New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast 20, 985 993.
19. Westermeyer, C. Macreadie, I.G. (2007) Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA in Candida glabrata. FEMS Yeast Res 7, 436 441.
20. Ying, S., Sun, Y.M., Liu, X.M., An, C.Y. Gao, Y.Y. (2007) Effect of ScrF I polymorphism in the 2nd intron of the HMGCR gene on lipid-lowering response to simvastatin in Chinese diabetic patients. Biochem Biophys Res Commun 363, 395 398.