Distinguishing between human and animal sources of fecal pollution in waters: A review

International Journal of Water

Volumn 5, Issue 1, 2009, Pages 15-34

  Tyagi, Punam ^a   Edwards, D R ^a   Coyne, M S ^b  

^a UNIVERSITY OF KENTUCKY   (United States)

^b UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Bacteriophages of bacteriodes; Bifidobacteria; F specific RNA coliphages; FC FS ratio; Fecal sterols and bile acid; Human enteric viruses

Indexed keywords

ALCOHOLS; ANIMALS; BACTERIOPHAGES; BIOMARKERS; CHEMICAL ANALYSIS;

BACTERIOPHAGES OF BACTERIODES; BIFIDOBACTERIA; F-SPECIFIC RNA COLIPHAGES; FC/FS RATIO; FECAL STEROLS AND BILE ACID; HUMAN ENTERIC VIRUS;

WATER POLLUTION;

BACTERIOPHAGE; BACTERIUM; BIOMARKER; FECES; IDENTIFICATION METHOD; POLLUTANT SOURCE; RNA; STATISTICAL ANALYSIS; VIRUS; WATER POLLUTION;

ANIMALIA; BACTEROIDES;

EID: 60049092501     PISSN: 14656620     EISSN: 17415322     Source Type: Journal    
DOI: 10.1504/IJW.2009.023080     Document Type: Review

References (115)

1. Abidi, S.L. (2001) 'Chromatographic analysis of plant sterols in foods and vegetable oils- review', J. Chromatogr. A, Vol. 935, pp.173-201.
2. Allsop, K. and Stickler, D.J. (1985) 'An assessment of Bacteroides fragilis group of organisms as indicators of human fecal pollution', J. Appl. Bacteriol., Vol. 58, pp.95-99.
3. Bakola-Christianopoulou, M.N., Apazidou, K.K. and Psarros, L. (1996) 'A new method of bile acid silylation for their GLCMS analysis', Appl. Organomet. Chem., Vol. 10, pp.531-535.
4. Batta, A.K., Salen, G., Rapole, K.R., Batta, M., Batta, P., Alberts, D. and Earnest D. (1999) 'Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool', J. Lipid Res., Vol. 40, pp.1148-1154.
5. Beekwilder, J., Nieuwenhuizen, R., Havelaar, A.H. and van Duin Leiden, J. (1996) 'An oligonucleotide hybridization assay for the identification and enumeration of F-specific RNA phages in surface water', J. Appl. Bacteriol., Vol. 80, pp.179-186.
6. Bethell, P.H., Goad, L.J., Evershed, R.P. and Ottaway, J. (1994) 'The study of molecular markers of human activity: the use of coprostanol in the soil as an indicator of human fecal material', J. Archaeol. Sci., Vol. 21, pp.619-632.
7. Blau K. and Halket, M. (Eds.) (1993) Handbook of Derivatives for Chromatography, John Wiley & Sons, Chichester, England.
8. Bull, I.D., Betancourt, P.P. and Evershed R.P. (1999) 'Chemical evidence for a structured agricultural manuring regime on the Island of Pseria, Crete during the Minoan period', Aegaeum., Vol. 20, pp.69-74.
9. Bull, I.D., Elhmmali, M.M., Roberts, D.J. and Evershed, R.P. (2003) 'The application of sterol biomarkers to track the abandonment of a Roman waste water course at the Agora (Athens, Greece)', Archaeometry, Vol. 45, No. 1, pp.149-161.
10. Bull, I.D., Lockheart, M.J., Elhmmali, M.M., Roberts, D.J. and Evershed, R.P. (2002) 'The origin of feces by means of biomarker detection', Environ. Int., Vol. 27, pp.647-654.
11. Carreira, R.S., Wagener, L.R. and Readman, J.W. (2004) 'Sterols as markers of sewage contamination in a tropical urban estuary (Guanabara Bay, Brazil): space- time variations', Estuar. Coast Mar. Sci., Vol. 60, pp.587-598.
12. Chaler, R., Simoneit, B.R.T., and Grimalt, J.O. (2001) 'Bile acids and sterols in urban sewage treatment plants', J. Chromatogr. A, Vol. 927, pp.155-160.
13. Chan, K., Lam, M.H.W., Poon, K., Yeung, H. and Chiu T.K.T. (1998) 'Application of sedimentary fecal stanols and sterols in tracing sewage pollution in coastal waters', Water Res., Vol. 32, No. 1, pp.225-235.
14. Chou, C.C., and Liu, Y.P. (2004) 'Determination of fecal sterols in the sediments of different waste water outputs by GC-MS', Int. J. Environ. An. Ch., Vol. 84, No. 5, pp.379-388.
15. Cofre, J., Blasi, M., Bosch, A. and Lucena F. (1989) 'Occurrence of bacteriophages infecting Bacteroides fragilis and other viruses in polluted marine sediments', Water Sci. Technol., Vol. 21, pp.15-19.
16. D'Herelle, F. (1926) The Bacteriophage and Its Behavior (English translation by G.H. Smith), Williams and Wilkins, Baltimore, MD.
17. Delille, D. (1987) 'Spatial distribution of coastal Antarctic seawater bacteria; relationship with Avifauna', Polar Biol., Vol. 8, pp.55-60.
18. Dhillon, T.S., Dhillon, E.K.S., Chau, H.C., Li, W.K., and Tsang, A.H.C. (1976) 'Studies on bacteriophages distribution - virulent and temperate bacteriophages content of mammalian feces', Appl. Environ. Microbiol., Vol. 32, pp.68-74.
19. Düreth, S., Reimer, H. and Pecher, K. (1986) 'Tracing fecal pollution by coprostanol and intestinal bacteria in an ice covered Finnish lake loaded with both industrial and domestic sewage', Water Air Soil Poll., Vol. 28, pp.131-149.
20. Dutka, B.j., Chau, A.S.Y. and Coburn, J. (1974) 'Relationship between bacterial indicators of water pollution and fecal sterols', Water Res., Vol. 8, pp.1047-1055.
21. Elhmmali, M.M. (1998) Complementary Use of Bile Acids and Sterols as Sewage Pollution Indicators, PhD Dissertation, University of Bristol, UK.
22. Elhmmali, M.M., Roberts, D. and Evershed, R.P. (1997) 'Bile acids as a new class of sewage pollution indicator', Environ. Sci. Technol., Vol. 31, pp.3663-3668.
23. Elhmmali, M.M., Roberts, D. and Evershed, R.P. (2000) 'Combined analysis of bile acids and sterols/stanols from riverine particulates to access sewage discharges and other fecal sources', Environ. Sci. Technol., Vol. 34, pp.39-46.
24. Elliott, W.H. (1980) 'Identification of sterols and bile acids by computerized gas chromatography-mass spectrometry', Lipids, Vol. 15, No. 9, pp764-769.
25. Enriquez, C.E., Hurst, C.J. and Gerba, C.P. (1995) 'Survival of the enteric adenoviruses 40 and 41 in tap, sea, and wastewater', Water Res., Vol. 29, pp.2548-2553.
26. Evershed, R.P. and Bethell, P.H. (1996) 'Application of multimolecular biomarker techniques to the identification of fecal material in archaeological soils and sediments', Acs. Sym. Ser., Vol. 625, pp.157-172.
27. Fattore, E., Benfenati, E., Marelli, R., Cools, E. and Fanelli, R. (1996) 'Sterols in sediment samples from Venice lagoon, Italy', Chemosphere, Vol. 33, pp.2383-2393.
28. Furuse, K. (1987) 'Distribution of coliphages in the environment: general consideration', Phage Ecology, Wiley Interscience, pp.87-124.
29. Gavini, F., Pourcher, A.M. and Neut, C. (1991) 'Phenotypic differentiation of bifidobacteria of human and animal origins', Int. J. Syst. Bacteriol., Vol. 41, pp.548-557.
30. Geldreich, E.E. (1976) 'Fecal coliform and fecal streptococcus density relationships in waste discharges and receiving waters', Critical Rev. Environ. Contr., Vol. 6, pp.349-369.
31. Geldreich, E.E. and Kenner, B.A. (1969) 'Concepts of fecal streptococci in stream pollution', J. Water Pollut. Contr. Fed., Vol. 41, Part 2, pp.R336-R352.
32. Gomez, D., Page, R.W., Holsing, N., Gassman, N. and Riley, G.F. (1998) Implementation of a Chemical Method for Differentiating Human and Animal Fecal Impacts in Surface Waters and Sediments, Department of Natural Resource Protection: Technical Report Series, TR: 98-01.
33. Goodfellow, R.M., Cardoso, J., Eglington, G., Dawson, J.P. and Best, G.A. (1977) 'A fecal sterol survey in the Clyde Estuary', Mar. Pollut. Bull., Vol. 8, pp.272-276.
34. Gordon, M.H. (1905) 'Report of some characters by which various streptococci and staphylococci may be differentiated and identified', Supplement to the 33rd Annual Report of the Local Government Board Containing the 'Report of the Medical Officer' for 1903-04, p.388.
35. Grabow, W.O.K., Neubrech, T.E., Holtzhausen, C.S. and Jofre, J. (1995) 'Bacteroides fragilis and Escherichia coli bacteriophages: excretion by humans and animals', Environ. Sci. Technol., Vol. 31, pp.223-230.
36. Griffin, D.W., Stokes, R., Rose, J.B. and Paul III, J.H. (2000) 'Bacterial indicator occurrences and the use of an F+specific RNA coliphages assay to identify fecal sources in Homosassa springs, Florida', Microbial. Ecol., Vol. 39, No. 1, pp.56-64.
37. Grimalt, J.O., Fernandez, P., Bayona, J.M. and Albalges, J. (1990) 'Assessment of fecal sterols and ketones as indicators of urban sewage inputs to coastal waters', Environ. Sci. Technol., Vol. 24, pp.357-363.
38. Gulacar, F.O., Sisini, A. and Klohn, M. (1989) 'Preservation and post-mortem transformations of lipids in samples from a 4000-year-old Nubian mummy', J. Archaeol. Sci., Vol. 17, pp.691-705.
39. Hakawaya, S. (1973) 'Microbial transformation of bile acids', Adv. Lipid Res., Vol. 11, pp.143-192.
40. Haslewood, G.A.D. (1967) Bile Salts, Methuem, London.
41. Havelaar, A.H. (1993) 'Bacteriophages as models of human enteric viruses in the environment', ASM News, Vol. 59, pp.614-619.
42. Havelaar, A.H. and Hogeboom, W.M. (1984) 'A method for the enumeration of male- specific bacteriophages in sewage', J. Appl. Bacteriol., Vol. 56, pp.439-447.
43. Havelaar, A.H., Furuse, K. and Hogeboom, W.M. (1986) 'Bacteriophages and indicator bacteria in human and animal feces', J. Appl. Bacteriol., Vol. 60, pp.255-262.
44. Havelaar, A.H., Pot-Hogeboom, W.M., Furuse, K., Pot, R. and Hormann, M.P. (1990) 'F-specific RNA bacteriophages and sensitive host strains in feces and wastewater of human and animal origin', J. Appl. Bacteriol., Vol. 69, pp.30-37.
45. Hirano, S., Nakama, R., Tamaki, M., Masuda, N. and Oda, H. (1981) 'Isolation and characterization of thirteen intestinal microorganisms capable of 7a-dehydroxylating the bile acids', Appl. Environ. Microbiol., Vol. 41, pp.737-745.
46. Hoving, E.B. (1995) 'Chromatopgraphic methods in the analysis of cholesterol and related lipids- review', J. Chromatogr. B, Vol. 671, pp.341-362.
47. Howell, J.M., Coyne, M.S. and Cornelius, P.L. (1995) 'Fecal bacteria in agricultural waters of the blue grass region of Kentucky', J. Environ. Qual., Vol. 24, pp.411-419.
48. Hsu, F.C., Shieh, Y.S.C., Duin, J.V., Beekwilder, M.J. and Sobsey, M.D. (1995) 'Genotyping male-specific RNA coliphages by hybridization with oligonucleotide probes', Appl. Environ. Microbiol., Vol. 61, pp.3960-3966.
49. Hughes, K.A. and Thompson, A. (2004) 'Distribution of sewage pollution around maritime Antarctic research station indicated by fecal coliforms, Clostridium perfringens and fecal sterol markers', Environ. Pollut., Vol. 127, pp.315-321.
50. Iida, T., Hikosaka, M., Goto, J. and Nambara, T. (2001) 'Capillary gas chromatographic behaviour of tert.-hydroxylated steroids by trialkylsilylation', Chromatogr. A, Vol. 937, Nos. 1-2, pp.97-105.
51. Iida, T., Momose, T., Tamura, T., Matsumoto, T., Goto, J., Nambara, T. and Chang, F.C. (1987) 'Capillary gas chromatography of hydroxylated bile acid stereoisomers of the allo and normal series', J. Chromatogr., Vol. 389, No. 1, pp.155-164.
52. Iida, T., Shiraishi, K., Ogawa, S., Goto J. and Nambara T. (2003) 'An improved method for the capillary gas chromatographic derivatization of polyhydroxylated steroids having tert-hydroxyl groups', Anal. Sci., Vol. 19, No. 9, pp.1317-1321.
53. Isobe, K.O., Tarao, M., Cheim, N.H., Minh, L.Y. and Takada, H. (2004) 'Effect of environmental factors on the relationship between concentrations of coprostanol and fecal sterols indicator bacteria in Tropical (Mekong Delta) and temperate (Tokyo) freshwaters', Appl. Environ. Microbiol., Vol. 70, No. 2, pp.814-821.
54. Isobe, K.O., Tarao, M., Zakaria, M.P., Cheim, N.H., Minh, L.Y. and Takada, H. (2002) 'Quantitative application of fecal sterols using gas chromatography- mass spectrometry to investigate fecal pollution in tropical waters: western Malaysia and Mekong Delta, Vietnam', Environ. Sci. Technol., Vol. 36, pp.4497-4507.
55. Jagals, P., Grabaw, W.O.K. and Villers, J.C.D. (1995) 'Evaluation of indicators for assessment of human and animal fecal pollution of surface run-off', Water Sci. Technol., Vol. 31, Nos. 5-6, pp.235-241.
56. Jeng, W., Wang, J. and Han, B. (1996) 'Coprostanol distribution in marine sediments off South-Western Taiwan', Environ. Pollut., Vol. 94, No. 1, pp.47-52.
57. Jiang, S., Noble, R. and Chu, W. (2001) 'Human adenoviruses and coliphages in urban runoff impacted coastal waters of Southern California', Appl. Environ. Microbiol., Vol. 67, No. 1, pp.179-184.
58. Jofre, J., Blasi, M., Bosch, A. and Lucena, F. (1989) 'Occurrence of bacteriophages infecting Bacteroides fragilis and other viruses in polluted marine sediments', Water Sci. Technol., Vol. 21, pp.15-19.
59. Knights, B.A., Dickson, C.A., Dickson, J.H. and Breeze D.J. (1983) 'Evidence concerning the Roman military diet at Bearsden, Scotland, in the 2nd century A.D.', J. Archaeol. Sci., Vol. 10, pp.139-152.
60. Leary, T.O., Leeming, R., Nichols, P.D. and Volkman, J.K. (1999) 'Assessment of the sources, transport and fate of sewage-derived organic matter in Port Phillip Bay, Australia, using the signature lipid coprostanol', Mar. Freshw. Res., Vol. 50, pp.547-556.
61. Leeming, R. and Coleman, R. (2001) Bayside Drains Fecal Origins Study, CSIRO Marine Research Report, No. FPP-02, Melbourne Water.
62. Leeming, R. and Nichols, P.D. (1996) 'Concentrations of coprostanol that correspond to existing bacterial indicator guideline limits', Water Res., Vol. 30, No. 12, pp.2997-3006.
63. Leeming, R., Ball, A., Ashbolt, N., Jones, G. and Nichols, P. (1994) 'Distinguishing between human and animal sources of fecal pollution', Chemistry in Australia, Aust. J. Chem., Vol. 61, pp.434-435.
64. Leeming, R., Ball, A., Ashbolt, N. and Nichols, P.D. (1996) 'Using fecal sterols from humans and animals to distinguish fecal pollution in receiving waters', Water Res., Vol. 30, No. 12, pp.2893-2900.
65. Leeming, R., Bate, N., Hwelett, R. and Nichols, P.D. (1998) 'Discriminating fecal pollution: a case study of storm water entering Port Phillip Bay, Australia', Water Sci. Technol., Vol. 38, No. 10, pp.15-22.
66. Leeming, R., Latham, V., Rayner, M. and Nichols P. (1997) 'Detecting and distinguishing sources of sewage pollution in Australian inland, coastal waters and sediments', ACS Symp. Ser., Vol. 671, pp.306-319.
67. Lin, D.S., Connor, W.E., Napton, L.K. and Heizer, R.F. (1978) 'The steroids of 2000-year-old human coprolites', J. Lipid. Res., Vol. 19, pp.215-221.
68. MacDonald, I.A., Bokkenheuser, V.D., Winter, J., McLernon, A.M. and Mosbach, E.H. (1983) 'Degradation of sterols in the human gut', J. Lipid Res., Vol. 24, pp.675-694.
69. Mackenzie, A.S., Brasell, S.C., Eglinton, G. and Maxwell, J.R. (1982) 'Chemical fossils: the geological fate of steroids', Science, Vol. 217, pp.491-504.
70. Mara, D.D. and Oragui, J.I. (1983) 'Sorbitol fermenting bifidobacteria as specific indicators of human fecal pollution', J. Appl. Bacteriol., Vol. 55, pp.349-357.
71. Marsheck, W.J., Kraychy, S. and Muir, R.D. (1972) 'Microbial degradation of sterols', Appl. Microbiol., Vol. 23, pp.72-77.
72. Matsuki, T., Watanabe, K., Tanaka, R. and Oyaizu, H. (1998) 'Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species and group specific primers', Fems. Microbiol. Lett., Vol. 167, pp.113-121.
73. McCalley, D., Cooke, M. and Nickless, G. (1981) Effect of sewage treatment of fecal sterols', Water Res., Vol. 15, pp.1019-1025.
74. Mossel, D.A.A. (1958) 'The suitability of bifidobacteria as part of a more extended bacterial association, indicating fecal contamination of foods', Proc. 7th Internat. Congr. Microbiol., p.440.
75. Mudge, S.M. and Bebianno, M.J. (1997) 'Sewage contamination following an accidental spillage in the Ria Formosa, Portugal', Mar. Pollut. Bull., Vol. 34, No. 3, pp.163-170.
76. Mudge, S.M. and Lintern, D.G. (1999) 'Comparison of sterol biomarkers for sewage with other measures in Victoria Harbour, B.C., Canada', Estuar. Coast Shelf S, Vol. 48, pp.27-38.
77. Mudge, S.M. and Seguel, C.G. (1999) 'Organic contamination of San Vicente Bay, Chile', Mar. Pollu.t Bull., Vol. 38, pp.1011-1021.
78. Nash, D., Leeming, R., Clemow, L., Hannah, M., Halliwell, D. and Allen, D. (2005) 'Quantitative determination of sterols and other alcohols in overland flow from grazing land and possible source materials', Water Res., Vol. 39, pp.2964-2978.
79. Nes, W.R. and Nes, W.D. (1980) Lipids in Evolution, Plenum Press, New York.
80. Nichols, P.D, Leeming, R., Rayner, M.S. and Latham, V. (1996) 'Use of capillary gas chromatography for measuring fecal derived sterols application to storm water, the sea surface microlayer, beach greases, regional studies, and distinguishing algal blooms and human and non-human sources of sewage pollution', J. Chromatogr. A, Vol. 733, pp.497-509.
81. Nichols, P.D., Leeming, R., Rayner, M.S., Latham, V., Ashbolt, N.J. and Turner, C. (1993) 'Comparison of the abundance of the fecal sterol coprostanol and fecal bacterial groups in inner-shelf waters and sediments near Sydney, Australia', J. Chromatogr., Vol. 643, pp.189-195.
82. Nishimura, M. and Koyama, T. (1977) 'The occurrence of stanols in various living organisms and the behavior of sterols in contemporary sediments', Geochimica et Cosmochimica Acta, Vol. 41, No. 3, pp.379-385.
83. Ogura, K. (1983) 'Fate of coprostanol, an index of fecal pollution, in Tokyo Bay', Chikyukagaku (Geochemistry), Vol. 17, pp.68-75.
84. Osawa, S., Furuse, K. and Watanabe, I. (1981) 'Distribution of ribonucleic acid coliphages in animals', Appl. Environ. Microbiol., Vol. 41, pp.164-168.
85. Pelczar Jr., M.J., Chan, E.C.S. and Krieg, N.R. (1988) Microbiology, 5th ed., McGraw-Hill, Singapore, p.416.
86. Peng, X., Zhang, G., Mai, B., Min, Y. and Wang Z. (2002) 'Spatial and temporal trend of sewage pollution indicated by coprostanol in Macao Estuary, southern China', Mar. Pollut. Bull., Vol. 45, pp.295-299.
87. Post, F.J., Allen, A.D. and Reid, T.C. (1967) 'Simple medium for the selective isolation of Bacteroides and related organisms, and their occurrence in sewage', Appl. Microbiol., Vol. 15, pp.213-218.
88. Pourcher, A.M., Devriese, L.A., Hernandez, J.F. and Delattre, J.M. (1991) 'Enumeration by a miniaturized method of E. coli, Streptococcus bovis, and enterococci as indicators of the origin of fecal pollution in waters', J. Appl. Bacteriol., Vol. 70, pp.525-530.
89. Puig, A., Queralt, N., Jofre, J. and Araujo, R. (1999) 'Diversity of Bacteroides fragilis strains in their capacity to recover phages from human and animal wastes and from fecally polluted wastewater', J. Appl. Microbiol., Vol. 65, pp.1772-1776.
90. Quemeneur, M. and Marty, Y. (1992) 'Sewage influence in a macrotidal estuary: fatty acids and sterol distributions', Estuar Coast Shelf S, Vol. 34, pp.347-363.
91. Quemeneur, M. and Marty, Y. (1994) 'Fatty acids and sterols in domestic waste waters', Water Res., Vol. 28, pp.1217-12226.
92. Reeves, A.D. and Patton, D. (2005) 'Fecal sterols as indicators of sewage contamination in estuarine sediments of the Tay Estuary, Scotland: an extended baseline survey', Hydrol. Earth Syst. Sc., Vol. 9, pp.81-94.
93. Resnick, I.G. and Levin, M.A. (1981) 'Assessment of bifidobacteria as indicators of human fecal pollution', Appl. Environ. Microbiol., Vol. 42, pp.433-438.
94. Rhodes, M.W. and Kator, H. (1994) 'Seasonal occurrence of mesophilic Aeromonas spp. as a function of biotype and water quality in temperate freshwater lakes', Water Res., Vol. 28, pp.2241-2251.
95. Rhodes, M.W. and Kator, H. (1999) 'Sorbitol-Fermenting bifidobacteria as indicators of diffuse human fecal pollution in estuarine watersheds', J. Appl. Microbiol., Vol. 87, pp.528-535.
96. Sassaroli, A., Santos, F.M., Harsi, C.M., Vieira, M.J., Garrafa, P. and Mehnert, D.U. (2000) 'One-year survey of hepatitis-A and adenoviruses in wastewater samples on Sao Paulo city, Brazil', 1st World Water Congr. Int. Water Assoc., Book 7 Health-Related Water Microbiology, p.158.
97. Seguel, C.G., Mudge, S.M., Salgado, C. and Toledo, M. (2001) 'Tracing sewage in the marine environment: altered signatures in Concepcion Bay, Chile', Water Res., Vol. 35, No. 17, pp.4166-4174.
98. Setchell, K.D.R., Lawson, A.M., Tanida, N. and Sjovall, J. (1983) 'General methods for the analysis of metabolic profiles of bile acids and related compounds in feces', J. Lipid Res., Vol. 24, pp.1085-1100.
99. Seurinck, S., Verdievel, M. Verstraete, W. and Siciliano, S.D. (2005) 'Microbial source tracking for identification of fecal pollution', Rev. Environ. Sci. Bio/Technol., Vol. 4, pp.19-37.
100. Shimada, K., Mitamura, K. and Higashi, T. (2001) 'Gas chromatography and high- performance liquid chromatography of natural steroids- review', J. Chromatogr. A, Vol. 935, pp.141-172.
101. Simpson, I.A., Van-Bergen, P.F., Perret, V., Elhmmali, M.M., Roberts, D.J. and Evershed, R.P. (1999) 'Lipid biomarkers of manuring practice in relict anthropogenic soils', The Holocene, Vol. 9, pp.223-229.
102. Sinton, L.W., Finlay, R.K. and Hannah, D.J. (1998) 'Distinguishing human from animal fecal contamination in water: a review', NZ J. Mar. Freshw. Res., Vol. 32, pp.323-348.
103. Smith, L.L., Kohler, D.P., Hempel, J.E. and van Lier, J.E. (1968) 'Sterol metabolism-IV. Microbial disposition of 5B-cholestan-3B-ol', Lipids, Vol. 3, p.301.
104. Stellwag, E.J. and Hylemon, P.B. (1979) '7a-Dehydroxylation of cholic acid and chenodeoxy cholic acid by Clostridium leptum', J. Lipid Res., Vol. 20, pp.325-333.
105. Sylvia, K. and Gerhard, J. (2004) 'Determination of underivatised sterols and bile acid trimethylsilyl ether methyl esters by gas chromatography-mass spectrometry-single ion monitoring in feces', J. Chromatogr. B, Vol. 813, Nos. 1-2, pp.99-207.
106. Tabak, H.H., Bloomhuff, R.N. and Bunch, R.L. (1972) 'Coprostanol: a positive tracer of fecal pollution', Dev. Ind. Microbiol., Vol. 13, pp.296-307.
107. Takada, H., Farringto, J.W., Brother, M.H., Johnson, C.H. and Tripp, B.W. (1994) 'Transport of sewage derived organic pollutants to deep-sea sediments at deep watered dump site-106', Environ. Sci. Technol., Vol. 28, pp.1062-1072.
108. Tartera, C. and Jofre, J. (1987) 'Bacteriophages active against Bacteroides fragilis in sewage-polluted waters', Appl. Environ. Microbiol., Vol. 53, pp.1632-1637.
109. Tyagi, P., Edwards, D.R. and Coyne, M.S. (2007) 'Use of selected chemical markers in combination with a multiple regression model to assess the contribution of domesticated animal sources of fecal pollution in the environment', Chemosphere, Vol. 69, No. 10, pp.1617-1624.
110. Venkatesan, M.I. and Kaplan, I.R. (1990) 'Sedimentary coprostanol as an index of sewage addition in Santa Monica Basin, Southern California', Environ. Sci. Technol., Vol. 24, No. 2, pp.208-214.
111. Venkatesan, M.I. and Santiago, C.A. (1989) 'Sterols in ocean sediments: novel tracers to examine habitats of cetaceans, pinnipeds, penguins and humans', Mar. Biol., Vol. 102, pp.431-437.
112. Venkatesan, M.I., Ruth, E. and Kaplan, I.R. (1986) 'Coprostanols in Antarctic marine sediments: a biomarker for marine mammals and non human pollution', Mar. Pollut. Bull., Vol. 17, pp.554-557.
113. Volin, P. (2001) 'Analysis of steroidal lipids by gas and liquid chromatography- review', J. Chromatogr. A, Vol. 935, pp.125-140.
114. Walker, R.W., Wun, C.K. and Litsky, W. (1982) 'Coprostanol as an indicator of fecal pollution', Crit. Rev. Env. Contr., Vol. 12, pp.91-112.
115. Wun, C.K., Wallker, W. and Litsky, W. (1976) 'The use of XAD-2 resin for the analysis of coprostanol in water', Water Res., Vol. 10, pp.955-959.