Studying the effect of single mismatches in primer and probe binding regions on amplification curves and quantification in real-time PCR

Journal of Microbiological Methods

Volumn 76, Issue 3, 2009, Pages 316-319

  Süß, Beate ^a   Flekna, Gabriele ^a   Wagner, Martin ^b   Hein, Ingeborg ^b  

^a Institute for Milk Hygiene   (Austria)

^b UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

Genetic instability; Mismatch; qPCR; Quantification; Real time PCR

Indexed keywords

BACTERIAL DNA;

ANALYTICAL ERROR; ARTICLE; BASE MISPAIRING; GENETIC STABILITY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; BASE PAIR MISMATCH; DNA PRIMERS; DNA, BACTERIAL; POLYMERASE CHAIN REACTION;

EID: 59849096286     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.12.003     Document Type: Article

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