Stable carbon isotope discrimination by human 3-hydroxy-3-methylglutaryl-coenzyme A reductase

Journal of Lipid Research

Volumn 49, Issue 12, 2008, Pages 2620-2626

  Lüdke, Svena M ^a   Flenker, Ulrich ^a   Schänzer, Wilhelm ^a   Schomburg, Dietmar ^b  

^a GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Curve fitting; Doping control; Isotope ratio mass spectrometry; Kinetic isotope effect; Mevalonate pathway; Steroids

Indexed keywords

CARBON; CARBON 13; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; MEVALONIC ACID; DRUG DERIVATIVE; MEVALONOLACTONE;

ARTICLE; COMBUSTION; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; FRACTIONATION; GAS CHROMATOGRAPHY; ISOTOPE ANALYSIS; LACTONIZATION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOLID PHASE EXTRACTION; CHEMISTRY; HUMAN; MASS FRAGMENTOGRAPHY;

CARBON ISOTOPES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; HYDROXYMETHYLGLUTARYL COA REDUCTASES; MEVALONIC ACID;

EID: 59649119616     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M800313-JLR200     Document Type: Article

References (29)

1. Cleland, W. W. 1982. Use of isotope effects to elucidate enzyme mechanisms. CRC Crit. Rev. Biochem. 13: 385-428.
2. O'Leary, M. H. 1981. Carbon isotope fractionation in plants. Phytochemistry. 20: 553-567.
3. Hayes, J. M. 2001. Fractionation of the Isotopes of Carbon and Hydrogen in Biosynthetic Processes. Vol. 43. Mineralogical Society of America, Washington DC. 225-277.
4. Niro, M. J. D., and S. Epstein. 1977. Mechanism of carbon isotope fractionation associated with lipid synthesis. Science. 197: 261-263.
5. Melzer, E., and H-L. Schmidt. 1987. Carbon isotope effects on the pyruvate dehydrogenase reaction and their importance for relative carbon-13 depletion in lipids. J. Biol. Chem. 262: 8159-8164.
6. 13C-values of endogenous urinary steroids. Steroids. 73: 408-416.
7. Goldstein, J. L., and M. S. Brown. 1990. Regulation of the mevalonate pathway. Nature. 343: 425-430.
8. Istvan, E. S., M. Palnitkar, S. K. Buchanan, and J. Deisenhofer. 2000. Crystal structure of the catalytic portion of human HMG-CoA reductase: insights into regulation of activity and catalysis. EMBO J. 19: 819-830.
9. Chatterjee, S., J. Schoepe, S. Lohmer, and D. Schomburg. 2005. High level expression and single-step purification of hexahistidine-tagged l-2-hydroxyisocaproate dehydrogenase making use of a versatile expression vector set. Protein Expr. Purif. 39: 137-143.
10. Mayer, R. J. 1988. Purification and properties of the catalytic domain of human 3-hydroxy-3-methylglutaryl-CoA reductase expressed in Escherichia coli. Arch. Biochem. Biophys. 267: 110-118.
11. Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62: 293-300.
12. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
13. Edwards, P. A., D. Lemongello, and A. M. Fogelman. 1979. Improved methods for the solubilization and assay of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase. J. Lipid Res. 20: 40-46.
14. Saini, G. S., T. A. Wani, A. Gautam, B. Varshney, T. Ahmed, K. S. Rajan, K. K. Pillai, and J. K. Paliwal. 2006. Validation of the LCMS/MS method for the quantification of mevalonic acid in human plasma and determination of the matrix effect. J. Lipid Res. 47: 2340-2345.
15. Flenker, U., M. Hebestreit, T. Piper, F. Hülsemann, and W. Schänzer. 2007. Improved performance and maintenance in gas chromatography/isotope ratio mass spectrometry by precolumn solvent removal. Anal. Chem. 79: 4162-4168.
16. Schimmelmann, A. H, C, N, O stable isotope reference materials from Indiana University, URL: http://php.indiana.edu/~aschimme/hc.html, Oct 2008.
17. Cleveland, W. S., E. Grosse, W. M. Shyu. Local Regression Models. In Chambers, J. M., and T. J. Hastie, editors. 1992. Statistical Models. S. Wadsworth & Brooks/Cole Computer Science, Pacific Grove, CA. Chapter 8.
18. Wu, J. T., L. H. Wu, and J. A. Knight. 1986. Stability of NADPH: effect of various factors on the kinetics of degradation. Clin. Chem. 32: 314-319.
19. R Development Core Team. 2008. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria.
20. Huber, P. J. 1981. Robust Statistics. John Wiley & Sons, Inc., New York.
21. Carpenter, J., and J. Bithell. 2000. Bootstrap confidence intervals: when, which, what? A practical guide for medical statisticians. Stat. Med. 19: 1141-1164.
22. O'Leary, M. H., H. Yamada, and C. J. Yapp. 1981. Multiple isotope effect probes of glutamate decarboxylase. Biochemistry. 20: 1476-1481.
23. Rosenberg, R. M., and M. H. O'Leary. 1985. Aspartate (beta)-decarboxylase from Alcaligenes faecalis: carbon-13 kinetic isotope effect and deuterium exchange experiments. Biochemistry. 24: 1598-1603.
24. Melzer, E., and H-L. Schmidt. 1988. Carbon isotope effect on the decarboxylation of carboxylic acids - comparison of the lactate oxidase reaction and the degradation of pyruvate by H2O2. Biochem. J. 252: 913-915.
25. Tong, J. Y., and P. E. Yankwich. 1957. Calculation of experimental isotope effects for pseudo first-order irreversible reactions. J. Phys. Chem. 61: 540-543.
26. O'Leary, M. H. 1980. Determination of heavy-atom isotope effects on enzyme-catalysed reactions. Methods Enzymol. 64: 83-104.
27. Monson, K. D., and L. M. Hayes. 1982. Carbon isotopic fractionation in the biosynthesis of bacterial fatty acids. Ozonolysis of unsaturated fatty acids as a means of determining the intramolecular distribution of carbon isotopes. Geochim. Cosmochim. Acta. 46: 139-149.
28. WADA Laboratory Committee. 2004. Reporting and Evaluation Guidance for Testosterone, Epitestosterone, T/E Ratio and Other Endogenous Steroids. World Anti Doping Agency, Montreal, WADA document TD2004EAAS.
29. Craig, H. 1953. The geochemistry of the stable carbon isotopes. Geochim. Cosmochim. Acta. 3: 53-92.