Simultaneous analysis of bacterioferritin gene expression and intracellular iron status in pseudomonas putida KT2440 by using a rapid dual luciferase reporter assay

Applied and Environmental Microbiology

Volumn 75, Issue 3, 2009, Pages 866-868

  Chen, Shicheng ^a   Bleam, William F ^a   Hickey, William J ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOFERRITIN; INTRACELLULAR IRONS; IRON CONCENTRATIONS; LUCIFERASE REPORTER ASSAYS; PSEUDOMONAS PUTIDA; RENILLA LUCIFERASE; RHIZOBACTERIUM; SIMULTANEOUS ANALYSIS;

ENZYME ACTIVITY; GENE EXPRESSION; PLANTS (BOTANY); TOXICITY;

IRON ANALYSIS;

BACTERIUM; BIOASSAY; CONCENTRATION (COMPOSITION); ENZYME ACTIVITY; FLY; GENE EXPRESSION; IRON; RHIZOBACTERIUM;

BACTERIAL PROTEINS; CYTOCHROME B GROUP; FERRITINS; GENE EXPRESSION PROFILING; GENES, REPORTER; IRON; LUCIFERASES; PSEUDOMONAS PUTIDA; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

PSEUDOMONAS PUTIDA; RENILLA LUCIFERASE; RHIZOBIUM;

EID: 59649088583     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01823-08     Document Type: Article

References (16)

1. Abdul-Tehrani, H., A. J. Hudson, Y.-S. Chang, A. R. Timms, C. Hawkins, J. M. Williams, P. M. Harrison, J. R. Guest, and S. C. Andrews. 1999. Ferritin mutants of Escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient. J. Bacteriol. 181:1415-1428.
2. Andrews, S. C. 1998. Iron storage in bacteria. Adv. Microb. Physiol. 40:281-351.
3. Andrews, S. C., N. E. Le Brun, V. Barynin, A. J. Thomson, G. R. Moore, J. R. Guest, and P. M. Harrison. 1995. Site-directed replacement of the coaxial heme ligands of bacterioferritin generate heme-free variants. J. Biol. Chem. 270:23268-23274.
4. Andrews, S. C., A. K. Robinson, and F. Rodriguez-Quinones. 2003. Bacterial iron homeostasis. FEMS Microbiol. Rev. 27:215-237.
5. Baaghil, S., A. Lewin, G. R. Moore, and N. E. Le Brun. 2003. Core formation in Escherichia coli bacterioferritin requires a functional ferroxidase center. Biochemistry 42:14047-14056.
6. Espinosa-Urgel, M., and J.-L. Ramos. 2004. Cell density-dependent gene contributes to efficient seed colonization by Pseudomonas putida KT2440. Appl. Environ. Microbiol. 70:5190-5198.
7. Espinosa-Urgel, M., A. Salido, and J.-L. Ramos. 2000. Genetic analysis of functions involved in adhesion of Pseudomonas putida to seeds. J. Bacteriol. 182:2363-2369.
8. Gauss, G. H., P. Benas, B. Wiedenheft, M. Young, T. Douglas, and C. M. Lawrence. 2006. Structure of the DPS-like protein from Sulfolobus solfatari- cus reveals a bacterioferritin-like dimetal binding site within a DPS-like dodecameric assembly. Biochemistry 45:10815-10827.
9. Harker, A. R., and L. H. Wullstein. 1985. Evidence for two nonidentical subunits of bacterioferritin from Azotobacter vinelandii. J. Bacteriol. 162:651-655.
10. Hunt, M. D., G. S. Pettis, and M. A. McIntosh. 1994. Promoterand operator determinants for Fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene system. J. Bacteriol. 176:3944-3955.
11. Ma, J.-F., U. A. Ochsner, M. G. Klotz, V. K. Nanayakkara, M. L. Howell, Z. Johnson, J. E. Posey, M. L. Vasil, J. J. Monaco, and D. J. Hassett. 1999. Bacterioferritin A modulates catalase A (KatA) activity and resistance to hydrogen peroxide in Pseudomonas aeruginosa. J. Bacteriol. 181:3730-3742.
12. Nancharaiah, Y. V., P. Wattiau, S. Wuertz, S. Bathe, S. V. Mohan, P. A. Wilderer, and M. Hausner. 2003. Dual labeling of Pseudomonas putida with fluorescent proteins for in situ monitoring of conjugal transfer of the TOL plasmid. Appl. Environ. Microbiol. 69:4846-4852.
13. Nelson, K. E., C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hilbert, V. A. P. Martins dos Santos, D. E. Fouts, S. R. Gill, M. Pop, M. Holmes, L. Brinkac, M. Beanan, R. T. DeBoy, S. Daugherty, J. Kolonay, R. Madupu, W. Nelson, O. White, J. Peterson, H. Khouri, I. Hance, P. C. Lee, E. Holtzapple, D. Scanlan, K. Tran, A. Moazzez, T. Utterback, M. Rizzo, K. Lee, D. Kosack, D. Moestl, H. Wedler, J. Lauber, D. Stjepandic, J. Hoheisel, M. Straetz, S. Heim, C. Kiewitz, J. Eisen, and K. N. Timmis. 2002. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ. Microbiol. 4:799-808.
14. Sherf, B. A., S. L. Navarro, R. R. Hannah, and K. V. Wood. 1996. Dualluciferase reporter assay: an advanced co-reporter technology integrating firefly and Renilla luciferase assays. Promega Notes 57:2-8.
15. Smith, J. L. 2004. The physiological role of ferritin-like compounds in bacteria. Crit. Rev. Microbiol. 30:173-185.
16. Weinel, C., K. E. Nelson, and B. Tummler. 2002. Global features of the Pseudomonas putida KT2440 genome sequence. Environ. Microbiol. 4:809-818.