Determination of triacylglycerols in butterfat by normal-phase HPLC and electrospray-tandem mass spectrometry

Lipids

Volumn 44, Issue 2, 2009, Pages 169-195

  Kalo, P ^a   Kemppinen, A ^a   Ollilainen, V ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Ion trap; Long , medium , short chain, and odd acyl carbon number triacylglycerols; Milk fat; Normal phase liquid chromatography; Positive electrospray ionization tandem mass spectrometry; Quantification of triacylglycerol species; Regioisomers

Indexed keywords

BUTYRIC ACID; HEXANOIC ACID; LONG CHAIN TRIACYLGLYCEROL; MEDIUM CHAIN TRIACYLGLYCEROL; SHORT CHAIN FATTY ACID; TRIACYLGLYCEROL;

ARTICLE; BUTTER; ELECTROSPRAY MASS SPECTROMETRY; FATTY ACID ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SPECIES IDENTIFICATION;

ANIMALS; BUTTER; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FATTY ACIDS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRIGLYCERIDES;

EID: 59449097712     PISSN: 00244201     EISSN: 15589307     Source Type: Journal    
DOI: 10.1007/s11745-008-3247-5     Document Type: Article

References (43)

1. RG Jensen 2002 The composition of bovine milk lipids: January 1995 to December 2000 J Dairy Sci 85 295 350
2. WC Breckenridge A Kuksis 1968 Specific distribution of short-chain fatty acids in molecular distillates of bovine milk fat J Lipid Res 9 388 393
3. L Marai WC Breckenridge A Kuksis 1969 Specific distribution of fatty acids in the milk fat triglycerides of goat and sheep Lipids 4 562 570
4. RE Pitas J Sampugna RG Jensen 1967 Triglyceride structure of cows' milk fat. Preliminary observations on the fatty acid compositions of positions 1, 2, and 3 J Dairy Sci 50 1332 1336
5. JJ Myher A Kuksis L Marai P Sandra 1988 Identification of the more complex triacylglycerols in bovine milk fat by gas chromatography-mass spectrometry using polar capillary columns J Chromatogr 452 93 118
6. P Kalo A Kemppinen 1993 Mass spectrometric identification of triacylglycerols of enzymatically modified butterfat separated on a polarizable phenylmethylsilicone column J Am Oil Chem Soc 70 1209 1217
7. A Kemppinen P Kalo 1993 Fractionation of the triacylglycerols of lipase-modified butter oil J Am Oil Chem Soc 70 1203 1207
8. A Kemppinen P Kalo 1998 Analysis of sn-1(3)- and sn-2-short-chain acyl isomers of triacylglycerols in butteroil by gas-liquid chromatography J Am Oil Chem Soc 75 91 100
9. + ions J Chromatogr A 1134 260 283
10. H Kallio P Laakso R Huopalahti RR Linko 1989 Analysis of butter fat triacylglycerols by supercritical fluid chromatography/electron impact mass spectrometry Anal Chem 60 698 700
11. P Laakso P Manninen 1997 Identification of milk fat triacylglycerols by capillary supercritical fluid chromatography-atmospheric pressure chemical ionization mass spectrometry Lipids 32 1285 1295
12. A Kuksis L Marai JJ Myher 1991 Reversed-phase liquid chromatography-mass spectrometry of complex mixtures of natural triacylglycerols with chloride-attachment negative chemical ionization J Chromatogr 588 73 87
13. C Maniongui C Gresti M Bugaut S Gauthier J Bezard 1991 Determination of bovine butterfat triacylglycerols by reversed-phase liquid chromatography and gas chromatography J Chromatogr 543 81 103
14. J Gresti M Bugaut C Maniongui J Bezard 1993 Composition of molecular species of triacylglycerols in bovine milk fat J Dairy Sci 76 1850 1869
15. JJ Myher A Kuksis L Marai 1993 Identification of the less common isologous short-chain triacylglycerols in the most volatile 2.5% molecular distillate of butter oil J Am Oil Chem Soc 70 1183 1191
16. L Marai A Kuksis JJ Myher 1994 Reversed-phase liquid chromatography-mass spectrometry of the uncommon triacylglycerol structures generated by randomization of butteroil J Chromatogr 672 87 99
17. GA Spanos RB Schwartz RB van Breemen CH Huang 1995 High-performance liquid chromatography with light-scattering detection and desorption chemical-ionization tandem mass spectrometry of milk fat triacylglycerols Lipids 30 85 90
18. HR Mottram RP Evershed 2001 Elucidation of the composition of bovine milk fat triacylglycerols using high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry J Chromatogr 926 239 253
19. P Kalo A Kemppinen V Ollilainen A Kuksis 2004 Regiospecific determination of short-chain triacylglycerols in butterfat by normal-phase HPLC with on-line electrospray-tandem mass spectrometry Lipids 39 915 928
20. RD Plattner K Payne-Wahl 1979 Separation of triglycerides by chain length and degree of unsaturation on silica HPLC columns Lipids 14 152 153
21. SH Rhodes AG Netting 1988 Normal-phase high-performance liquid chromatography of triacylglycerols J Chromatogr 448 135 143
22. JT Lin 2007 HPLC separation of acyl lipid classes J Liq Chrom Relat Technol 30 2005 2020
23. TJ Mangos KC Jones TA Foglia 1999 Normal-phase high performance liquid chromatographic separation and characterization of short- and long-chain triacylglycerols Chromatographia 49 363 368
24. P Kalo A Kemppinen V Ollilainen A Kuksis 2003 Analysis of regioisomers of short-chain triacylglycerols by normal-phase liquid chromatography-electrospray tandem mass spectrometry Int J Mass Spectrom 229 167 180
25. P Kalo V Ollilainen JM Rocha FX Malcata 2006 Identification of simple lipids by normal-phase liquid chromatography-positive electrospray tandem mass spectrometry, and application of developed methods in comprehensive analysis of low erucic acid rapeseed oil lipids Int J Mass Spectrom 254 106 121
26. KL Duffin JD Henion JJ Shieh 1991 Electrospray and tandem mass spectrometric characterization of acylglycerol mixtures that are dissolved in nonpolar solvents Anal Chem 63 1781 1788
27. C Cheng ML Gross E Pittenauer 1998 Complete structural elucidation of triacylglycerols by tandem sector mass spectrometry Anal Chem 70 4417 4426
28. n ion-trap MS J Am Oil Chem Soc 80 195 202
29. AM McAnoy CC Wu RC Murphy 2005 Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap J Am Soc Mass Spectrom 16 1498 1509
30. RA Hites 1970 Quantitative analysis of triglyceride mixtures by mass spectrometry Anal Chem 42 1736 1740
31. WM Lauer AJ Aasen G Graff RT Holman 1970 Mass spectrometry of triglycerides: I. Structural effects Lipids 5 861 868
32. E Schulte M Höhn U Rapp 1981 Mass spectrometric determination of triglyceride patterns of fats by the direct chemical ionization technique (DCI) Fresenius Z Anal Chem 307 115 119
33. JJ Myher A Kuksis L Marai F Manganaro 1984 Quantitation of natural triacylglycerols by reversed-phase liquid chromatography with direct liquid inlet mass spectrometry J Chromatogr 283 289 301
34. H Kallio G Currie 1993 Analysis of low erucic acid turnip rapeseed oil (Brassica campestris) by negative ion chemical ionization tandem mass spectrometry. A method giving information on the fatty acid composition in positions sn-2 and sn-1/3 of triacylglycerols Lipids 28 207 215
35. WC Byrdwell EA Emken WE Neff RO Adlof 1996 Quantitative analysis of triglycerides using atmospheric pressure chemical ionization-mass spectrometry Lipids 31 919 935
36. WC Byrdwell WE Neff 1996 Analysis of genetically modified Canola varieties by atmospheric pressure chemical ionization mass spectrometric and flame ionization detection J Liq Chrom Relat Technol 19 2203 2225
37. PW Parodi 1975 Detection of acetodiacylglycerols in milk fat lipids by thin-layer chromatography J Chromatogr 111 1975 223 226
38. Y Itabashi JJ Myher A Kuksis 1993 Determination of positional distribution of short-chain fatty acids in bovine milk fat on chiral columns J Am Oil Chem Soc 70 1177 1181
39. JK Limb YH Kim SY Han GJ Jhon 1999 Isolation and characterization of monoacetyldiglycerides from bovine udder J Lipid Res 40 2169 2176
40. PW Hendrikse JL Harwood 1986
41. X Han RW Gross 2001 Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry Anal Biochem 295 88 100
42. WW Christie 1985 Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light scattering) detection J Lipid Res 26 507 512
43. Harris DC (1987) Quantitative chemical analysis. W. H. Freeman and Company, New York