Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 3, 2009, Pages 870-874

  Minami, Atsushi ^a   Tajima, Naoto ^a   Higuchi, Yusuke ^b   Toyomasu, Tomonobu ^c   Sassa, Takeshi ^c   Kato, Nobuo ^b   Dairi, Tohru ^a  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c YAMAGATA UNIVERSITY   (Japan)

Author keywords

Biosynthesis; Brassicicene C; Diterpene

Indexed keywords

BRASSICICENE C; DITERPENE; FUSICOCCADIENE SYNTHASE; METHYLTRANSFERASE; SYNTHETASE; UNCLASSIFIED DRUG;

ALTERNARIA; ALTERNARIA BRASSICICOLA; ARTICLE; ENZYME ACTIVITY; FUNGAL STRAIN; GENE CLUSTER; GENE FUNCTION; GENETIC ENGINEERING; GENOME; NONHUMAN; OPEN READING FRAME;

ALTERNARIA; CELL-FREE SYSTEM; CHEMISTRY, PHARMACEUTICAL; CHROMATOGRAPHY, GAS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA; DITERPENES; DRUG DESIGN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENES, BACTERIAL; GENOME; METHYLTRANSFERASES; MODELS, CHEMICAL; MODELS, GENETIC; MULTIGENE FAMILY; PLANT EXTRACTS;

ALTERNARIA BRASSICICOLA;

EID: 58849138249     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2008.11.108     Document Type: Article

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24. The coding sequences of orf6 and orf8 from A. brassicicola ATCC 96836 was deposited at GeneBank under Accession No. AB465603 and AB465604, respectively.
25. The orf8 gene was amplified by PCR from previously prepared first-strand cDNA with primers, 5′-GGGGGATCCAAATACCAATTTTCCATCATTGTGGA-3′ and 5′-ACGCGTCGACTCAAAGCTTGAGCATCATTAGCATC-3′. The subcloned PCR product was ligated into the pQE30 (QIAGEN) to give pQE30-orf8. The pQE30-orf8 was transformed into E. coli M15 for overexpression. In vivo analysis of fusicoccadiene was performed according to Ref. 18.
26. The orf6 gene was amplified by PCR from previously prepared first-strand cDNA with primers, 5′-GGGGGATCCGCAGTCCAAGAGACAGACCTGGACAA-3′ and 5′-ACGCGTCGACTTATGCATTCTGTGCCGCAGGCTTA-3′. The subcloned PCR product was ligated into the pQE30 (QIAGEN) to give pQE30-orf6. The pQE30-orf6 was transformed into E. coli M15 for overexpression. The reaction mixture (20 mM of MOPS (pH 6.9), 10% of glycerol, 0.5 mM of FC H aglycon, 1 mM of S-adenosyl l-methionin) with cell-free extract, prepared from E. coli cells having pQE30-orf6, was incubated in 30 °C. After 20 h, the reaction was terminated by addition of ethyl acetate, and the mixture was extracted with ethyl acetate. The combined organic layer was concentrated, and the residue was dissolved in 30 μL of methanol. The sample was analyzed by HPLC.