Investigation of the morphology, viability and mechanical properties of yeast cells in environmental SEM

Scanning

Volumn 30, Issue 6, 2008, Pages 435-442

  Ren, Yilong ^a   Donald, Athene M ^b   Zhang, Zhibing ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

ESEM; Mechanical properties; Nano manipulation; Radiation damage; Yeast cells

Indexed keywords

ACCELERATING VOLTAGES; BEAM CURRENTS; BIOLOGICAL CELLS; ELECTRON BEAM RADIATIONS; ENVIRONMENTAL SCANNING ELECTRON MICROSCOPIES; ESEM; EXPOSURE-TIME; HIGH VACUUMS; LOW VACUUMS; MATHEMATICAL MODELING; MEASUREMENT DEVICES; NANO-MANIPULATION; NANO-SCALE; SEM; SPOT SIZES; STRESS-STRAIN RELATIONSHIPS; TIME FRAMES; YEAST CELLS;

CELLS; CYTOLOGY; ELECTRON BEAMS; ELECTRON GUNS; FORCE MEASUREMENT; FUNCTIONS; MECHANICAL PROPERTIES; MORPHOLOGY; NANOTECHNOLOGY; PARTICLE BEAMS; RADIATION; RADIATION DAMAGE; SCANNING ELECTRON MICROSCOPY; STRESS-STRAIN CURVES; VACUUM; YEAST;

BIOMECHANICS;

ARTICLE; CELL DAMAGE; CELL STRUCTURE; CELL VIABILITY; DEVICE; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; YEAST CELL;

CALIBRATION; ENVIRONMENT; GLASS; MICROMANIPULATION; MICROSCOPY, ELECTRON, SCANNING; RADIATION DOSAGE; SACCHAROMYCES CEREVISIAE; SPECIMEN HANDLING; STRESS, MECHANICAL; TRANSDUCERS; VACUUM;

EID: 58849105853     PISSN: 01610457     EISSN: None     Source Type: Journal    
DOI: 10.1002/sca.20126     Document Type: Article

References (21)

1. Bayrock D, Ingledew WM: Fluidized bed drying of baker's yeast: moisture levels, drying rates, and viability changes during drying. Food Res Int 30, 407-415 (1997).
2. Campbell B: Cell-disruption method optimizes cell lysis-technical note: high-pressure yeast and bacterial cell disruption. Genet Eng News 23, 52-53 (2003).
3. Cerrutti P, de Huergo MS, Galvagno M, Schebor C, Buera MD: Commercial baker's yeast stability as affected by intracellular content of trehalose, dehydration procedure and the physical properties of external matrices. Appl Microbiol Biotechnol 54, 575-580 (2000).
4. Donald AM, Baker FS, Smith AC, Waldron KW: Fracture of plant tissues and walls as visulized by environmental scanning electron microscopy. Ann Bot 92, 73-77 (2003).
5. Kitching S, Donald AM: Beam damage of polypropylene in the environmental scanning electron microscope: an FTIR study. J Microsc 190, 357-365 (1998).
6. Kucsera J, Yarita K, Takeo K: Simple detection method for distinguishing dead and living yeast colonies. J Micro-biological Methods 41, 19-21 (2000).
7. Liu KK, Williams DR, Briscoe BJ: Compressive deformation of a single mcirocapsule. Phys Rev E 54, 6673-6680 (1996).
8. Liu T, Donald AM, Zhang Z: Novel manipulation in environmental scanning electron microscope for measuring mechanical properties of single nanoparticles. Mater Sci Technol 21, 289-294 (2005).
9. Mashmoushy H, Zhang Z, Thomas CR: Micromanipulation measurement of the mechanical properties of baker's yeast cells. Biotechnol Tech 12, 925-929 (1998).
10. McKinlay KJ, Scotchford CA, Grant DM, Oliver JM, King JR, et al: Scanning electron microscopy of biomaterials. Electron Microsc Anal Issue 179, 87-90 (2004).
11. Middelberg APJ: Process-scale disruption of microorganisms. Biotechnol Adv 13, 491-551 (1995).
12. Misirli Z, Oner ET, Kirdar B: Real imaging and size values of Saccharomyces cerevisiae cells with comparable contrast tuning to two environmental scanning electron microscopy modes. Scanning 29, 11-19 (2007).
13. Muscariello L, Rosso F, Marino G, Giordano A, Barbarisi M, et al: A critical overview of ESEM applications in the biological field. J Cell Physiol 205, 328-334 (2005).
14. Ren Y, Donald AM, Zhang Z: Investigation of the radiation damage to microcapsules in an environmental SEM. Mater Sci Technol 23, 857-864 (2007).
15. Royall CP, Thiel BL, Donald AM: Radiation damage of water in environmental scanning electron microscopy. J Microsc 204, 185-195 (2001).
16. Simonin H, Beney L, Gervais P: Sequence of occurring damages in yeast plasma membrane during dehydration and rehydration: mechanisms of cell death. Biochim Biophys Acta 1768, 1600-1610 (2007).
17. Smith AE, Zhang Z, Thomas CR: Wall material properties of yeast cells. Part I. Cell measurements and compression experiments. Chem Eng Sci 55, 2031-2041 (2000a).
18. Smith AE, Zhang Z, Thomas CR, Moxham KE, Middelberg APJ: Mechanical properties of Saccharomyces cerevisiae. Proc Nat Acad Sci USA 97, 9871-9874 (2000b).
19. Stokes DJ, Rea SM, Best SM, Bonfield W: Electron microscopy of mammalian cells in the absence of fixing, freezing, dehydration, or specimen coating. Scanning 25, 181-184 (2003).
20. Thomas CR, Zhang Z: The effect of hydrodynamics on biological materials. In Advances in Bioprocess Engineering, Vol. 2 (Galindo E, Ramirez OT, Eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, 137-170. ISBN 0-7923-4923-7 (1998).
21. Tseng AA: Recent developments in micromilling using focused ion beam technology. J Micromech Microeng 14, R15-R34 (2004).