A tetracycline repressor-based mammalian two-hybrid system to detect protein-protein interactions in vivo

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 129-131

  Thibodeaux, Gabrielle Nina ^a   Cowmeadow, Roshani ^a   Umeda, Aiko ^b   Zhang, Zhiwen ^a,b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b The University of Texas at Austin   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID SYSTEMS; MAMMALS;

GENETIC SELECTION; IN-VIVO; MAMMALIAN CELLS; PROTEIN-PROTEIN INTERACTIONS; SMALL MOLECULES; TWO-HYBRID SYSTEM;

PROTEINS;

TETRACYCLINE;

ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; EMBRYO; GENETIC SELECTION; HUMAN; HUMAN CELL; IN VIVO STUDY; MAMMAL CELL; MOLECULE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM; REPRESSOR GENE; TWO HYBRID SYSTEM;

MAMMALIA;

EID: 58749101484     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.11.042     Document Type: Article

References (20)

1. Phizicky E.M., and Fields S. Protein-protein interactions: methods for detection and analysis. Microbiol. Rev. 59 (1995) 94-123
2. Fields S., and Song O.-k. A novel genetic system to detect protein-protein interactions. Nature 340 (1989) 245-246
3. Aravind L., Dixit V.M., and Koonin E.V. Apoptotic molecular machinery: vastly increased complexity in vertebrates revealed by genome comparisons. Science 291 (2001) 1279-1284
4. Luo Y., Batalao A., Zhou H., and Zhu L. Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system. Biotechniques 22 (1997) 350-352
5. Michnick S.W., Ear P.H., Manderson E.N., Remy I., and Stefan E. Universal strategies in research and drug discovery based on protein-fragment complementation assays. Nat. Rev. Drug Discov. 6 (2007) 569-582
6. Hillen W., and Berens C. Mechanisms underlying expression on tn10 encoded tetracycline resistance. Annu. Rev. Microbiol. 48 (1994) 345-369
7. Schnappinger D., Schubert P., Berens C., Pfleiderer K., and Hillen W. Solvent-exposed residues in the tet repressor (tetr) four-helix bundle contribute to subunit recognition and dimer stability. J. Biol. Chem. 274 (1999) 6405-6410
8. Baron U., Gossen M., and Bujard H. Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res. 25 (1997) 2723-2729
9. Triezenberg S.J., Kingsbury R.C., and McKnight S.L. Functional dissection of vp16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 2 (1988) 718-729
10. Gossen M., and Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89 (1992) 5547-5551
11. Flint J., and Shenk T. Viral transactivating proteins. Annu. Rev. Genet. 31 (1997) 177-212
12. Henssler E.-M., Bertram R., Wisshak S., and Hillen W. Tet repressor mutants with altered effector binding and allostery. FEBS J. 272 (2005) 4487-4496
13. Wendt H., Baici A., and Bosshard H.R. Mechanism of assembly of a leucine zipper domain. J. Am. Chem. Soc. 116 (1994) 6973-6974
14. Dragan A.I., Frank L., Liu Y., Makeyeva E.N., Crane-Robinson C., and Privalov P.L. Thermodynamic signature of gcn4-bzip binding to DNA indicates the role of water in discriminating between the ap-1 and atf/creb sites. J. Mol. Biol. 343 (2004) 865-878
15. Hu J.C., O'shea E.K., Kim P.S., and Sauer R.T. Sequence requirements for coiled-coils: analysis with lambda repressor-gcn4 leucine zipper. Science 250 (1990) 1400-1403
16. Heuer K.H., Mackay J.P., Podzebenko P., Bains N., Weiss A.S., King G.F., and Easterbrook-Smith S.B. Development of a sensitive peptide-based immunoassay: Application to detection of the jun and fos oncoproteins. Biochemistry 35 (1996) 9069-9075
17. Schuermann M., Neuberg M., Hunter J.B., Jenuwein T., Ryseck R.-P., Bravo R., and Muller R. The leucine repeat motif in fos protein mediates complex formation with jun/ap-1 and is required for transformation. Cell 56 (1989) 507-516
18. Oyama R., Takashima H., Yonezawa M., Doi N., Miyamoto-Sato E., Kinjo M., and Yanagawa H. Protein-protein interaction analysis by c-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy. Nucleic Acids Res. 34 (2006) e102
19. Lu C., Zhu J., Wang Y., Umeda A., Cowmeadow R.B., Lai E., Moreno G.N., Person M.D., and Zhang Z. Staphlococcus aureus sortase a exists as a dimeric protein in vitro. Biochemistry 42 (2007) 9346-9354
20. Zhu J., Lu C., Standland M., Lai E., Moreno G.N., Umeda A., Jia X., and Zhang Z. Single mutation on the surface of staphylococcus aureus sortase a can disrupt its dimerization. Biochemistry 47 (2008) 1667-1674