Native promoter reporters validate transcriptional targets

Methods in Molecular Biology

Volumn 468, Issue , 2008, Pages 111-128

  Schmalhofer, Otto ^a   Spaderna, Simone ^a   Brabletz, Thomas ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Bacmid; Firefly luciferase; Native promoter regions; Reporter plasmids; TCF recognition sites; Wnt pathway

Indexed keywords

BETA CATENIN; MATRILYSIN; T CELL FACTOR PROTEIN; WNT PROTEIN;

ARTICLE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEIC ACID DATABASE; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROMOTER REGION; REPORTER GENE; REPRODUCIBILITY; SIGNAL TRANSDUCTION;

BASE SEQUENCE; BETA CATENIN; DATABASES, NUCLEIC ACID; GENE EXPRESSION REGULATION; GENES, REPORTER; HUMANS; MATRIX METALLOPROTEINASE 7; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS; SIGNAL TRANSDUCTION; TCF TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; WNT PROTEINS;

EID: 58749085425     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-249-6_9     Document Type: Article

References (12)

1. Giles, R.H., van Es, J.H., Clevers, H. (2003) Caught up in a Wnt storm: Wnt signaling in cancer. Biochim Biophys Acta 1653, 1-24.
2. van de Wetering, M., Sancho, E., Verweij, C., de Lau, W., Oving, I., Huristone, A., van der Horn, IC, Batlle, E., Coudreuse, D., Haramis, A.P., Tjon-Pon-Fong, M., Moerer, P., van den Born, M., Soete, G., Pals, S., Eilers, M., Medema, R., Clevers, H. (2002) The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111, 241-250.
3. Zirn, B., Samans, B., Wittmann, S., Pietsch, T., Leuschner, I., Graf, N., Gessler, M. (2006) Target genes of the WNT/betacatenin pathway in Wilms tumors. Genes Chromosomes Cancer 45, 565-574.
4. Huang, S., Li, Y., Chen, Y., Podsypanina, IC, Chamorro, M., Olshen, A.B., Desai, ICV, Tann, A., Petersen, D., Green, J.E., Varmus, H.E. (2005) Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice. Genome Biol 6, R84.
5. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press.
6. Brabletz, T., Jung, A., Dag, S., Hlubek, F., Kirchner, T. (1999) beta-catenin regulates the expression of the matrix metalloproteinase-7 in human colorectal cancer. Amer J Pathol 155, 1033-1038.
7. Crawford, H.C., Fingleton, B.M., Rudolph-Owen, L.A., Goss, K.J., Rubin-feld, B., Polakis, P., Matrisian, L.M. (1999) The metalloproteinase matrilysin is a target of beta-catenin transactivation in intestinal tumors. Oncogene 18, 2883-2891.
8. Korinek, V., Barker, N., Morin, P.J., van Wichen, D., de Weger, R., Kinzler, K.W., Vogelstein, B., Clevers, H. (1997) Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma. Science 275, 1784-1787.
9. Ghiselli, G., Coffee, N., Munnery, C.E., Koratkar, R., Siracusa, L.D. (2003) The cohesin SMC3 is a target for beta-catenin/ TCF4 transactivation pathway. J Biol Chem 278, 20259-20267.
10. Chakrabarti, R., Schutt, C.E. (2002) Novel sulfoxides facilitate GC-rich template amplification. Biotechniques 32, 866, 868, 870-862, 874.
11. Rockman, S.P., Currie, S.A., Ciavarella, M., Vincan, E., Dow, C., Thomas, R.J., Phillips, WA. (2001) Id2 is a target of the beta-cat - enin/T cell factor pathway in colon carcinoma. J Biol Chem 276, 45113-45119.
12. Gradl, D., Kuhl, M., Wedlich, D. (1999) The Wnt/Wg signal transducer beta-catenin controls fibronectin expression. Mol Cell Biol 19, 5576-5587.