Expression and functional characterization of choline transporter in human keratinocytes

Journal of Pharmacological Sciences

Volumn 109, Issue 1, 2009, Pages 102-109

  Uchida, Yoshihiro ^a   Inazu, Masato ^a,b   Takeda, Hiroshi ^a   Yamada, Tomoko ^a   Tajima, Hirohisa ^a   Matsumiya, Teruhiko ^a,b  

^a Department of Pharmacology ^*  (Japan)

^b TOKYO MEDICAL UNIVERSITY   (Japan)

Author keywords

Acetylcholine; Cell proliferation; Choline; Keratinocyte; Transporter

Indexed keywords

CARRIER PROTEIN; CATION; CHOLINE; CHOLINE TRANSPORTER LIKE PROTEIN 1; CHOLINE TRANSPORTER LIKE PROTEIN 1A; CHOLINE TRANSPORTER LIKE PROTEIN 1B; CHOLINE TRANSPORTER LIKE PROTEIN 1C; MESSENGER RNA; POTASSIUM ION; SODIUM ION; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; KERATINOCYTE; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MICHAELIS CONSTANT; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 58649108306     PISSN: 13478613     EISSN: 13478648     Source Type: Journal    
DOI: 10.1254/jphs.08291FP     Document Type: Article

References (27)

1. Grando SA, Kist DA, Qi M, Dahl MV. Human keratinocytes synthesize, secrete, and degrade acetylcholine. J Invest Dermatol. 1993;101:32-36.
2. Nguyen VT, Arredondo J, Chernyavsky AI, Kitajima Y, Grando SA. Keratinocyte acetylcholine receptors regulate cell adhesion. Life Sci. 2003;72:2081-2085.
3. Kurzen H, Wessler I, Kirkpatrick CJ, Kawashima K, Grando SA. The non-neuronal cholinergic system of human skin. Horm Metab Res. 2007;39:125-135.
4. Gordon PR, Gelman LK, Gilchrest BA. Demonstration of a choline requirement for optimal keratinocyte growth in a defined culture medium. J Nutr. 1988;118:1487-1494.
5. Apparsundaram S, Ferguson SM, George AL Jr, Blakely RD. Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. Biochem Biophys Res Commun. 2000;276:862-867.
6. Okuda T, Haga T, Kanai Y, Endou H, Ishihara T, Katsura I. Identification and characterization of the high-affinity choline transporter. Nat Neurosci. 2000;3:120-125.
7. Pfeil U, Haberberger RV, Lips KS, Eberling L, Grau V, Kummer W. Expression of the high-affinity choline transporter CHT1 in epithelia. Life Sci. 2003;72:2087-2090.
8. Proskocil BJ, Sekhon HS, Jia Y, Savchenko V, Blakely RD, Lindstrom J, et al. Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells. Endocrinology. 2004;145:2498-2506.
9. Haberberger RV, Pfeil U, Lips KS, Kummer W. Expression of the high-affinity choline transporter, CHT1, in the neuronal and non-neuronal cholinergic system of human and rat skin. J Invest Dermatol. 2002;119:943-948.
10. +-independent systems of choline transport by plasma membrane vesicles of A549 cell line. Am J Physiol. 1994;267: C1279-C1287.
11. Koepsell H, Schmitt BM, Gorboulev V. Organic cation transporters. Rev Physiol Biochem Pharmacol. 2003;150:36-90.
12. Gründemann D, Schechinger B, Rappold GA, Schömig E. Molecular identification of the corticosterone-sensitive extra- neuronal catecholamine transporter. Nat Neurosci. 1998;1:349-351.
13. Gründemann D, Liebich G, Kiefer N, Köster S, Schömig E. Selective substrates for non-neuronal monoamine transporters. Mol Pharmacol. 1999;56:1-10.
14. Kekuda R, Prasad PD, Wu X, Wang H, Fei YJ, Leibach FH, et al. Cloning and functional characterization of a potential-sensitive, polyspecific organic cation transporter (OCT3) most abundantly expressed in placenta. J Biol Chem. 1998;273:15971-15979.
15. O'Regan S, Traiffort E, Ruat M, Cha N, Compaore D, Meunier FM. An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins. Proc Natl Acad Sci USA. 2000;97:1835-1840.
16. +-independent choline transporter in rat astrocytes. J Neurochem. 2005;94:1427-1437.
17. Traiffort E, Ruat M, O'Regan S, Meunier FM. Molecular characterization of the family of choline transporter-like proteins and their splice variants. J Neurochem. 2005;92:1116-1125.
18. Cheng Y, Prusoff WH. Relationship between the inhibition constant (Ki) and the concentration of inhibitor which causes 50 per cent inhibition (IC50) of an enzymatic reaction. Biochem Pharmacol. 1973;22:3099-3108.
19. Imokawa G, Abe A, Jin K, Higaki Y, Kawashima M, Hidano A. Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin? J Invest Dermatol. 1991;96:523-526.
20. Wessler I, Reinheimer T, Kilbinger H, Bittinger F, Kirkpatrick CJ, Saloga J, et al. Increased acetylcholine levels in skin biopsies of patients with atopic dermatitis. Life Sci. 2003;72:2169-2172.
21. Friedrich A, George RL, Bridges CC, Prasad PD, Ganapathy V. Transport of choline and its relationship to the expression of the organic cation transporters in a rat brain microvessel endothelial cell line (RBE4). Biochim Biophys Acta. 2001;1512:299-307.
22. Hoffmann K, Grafe F, Wohlrab W, Neubert RH, Brandsch M. Functional characterization of a high-affinity choline transport system in human keratinocytes. J Invest Dermatol. 2002;119:118-121.
23. Koepsell H, Endou H. The SLC22 drug transporter family. Pflugers Arch. 2004;447:666-676.
24. Urakami Y, Okuda M, Masuda S, Saito H, Inui KI. Functional characteristics and membrane localization of rat multispecific organic cation transporters, OCT1 and OCT2, mediating tubular secretion of cationic drugs. J Pharmacol Exp Ther. 1998;287:800-805.
25. +-independent choline transport in primary cultures of neurons from mouse cerebral cortex. Neurosci Lett. 2006;393:216-221.
26. Scott A. Acetylcholine in normal and diseased skin. Br J Dermatol. 1962;74:317-322.
27. Shelly WB, Shelly ED, Ho AKS. Cholinergic urticaria: acetylcholine receptor-dependent immediate-type hypersensitivity reaction to copper. Lancet. 1983;I:843-846.