Effect of phenyl sepharose ligand density on protein monomer/aggregate purification and separation using hydrophobic interaction chromatography

Journal of Chromatography A

Volumn 1216, Issue 6, 2009, Pages 902-909

  McCue, Justin T ^a   Engel, Philip ^a   Thömmes, Jörg ^a  

^a BIOGEN   (United States)

Author keywords

Adsorption isotherm; Aggregate removal; Hydrophobic interaction chromatography; Ligand density; Phenyl sepharose; Preparative chromatography; Protein purification

Indexed keywords

HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LIGAND DENSITY; PHENYL SEPHAROSE; PREPARATIVE CHROMATOGRAPHY; PROTEIN PURIFICATION;

ADSORBENTS; ADSORPTION; ADSORPTION ISOTHERMS; AGGREGATES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; FLOW INTERACTIONS; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; ISOTHERMS; LIGANDS; PURIFICATION; SPECIFICATIONS;

HYDROPHOBIC CHROMATOGRAPHY;

ADSORBENT; MONOMER; PHENYL SEPHAROSE; SEPHAROSE; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; DENSITY; ISOTHERM; LARGE SCALE PRODUCTION; PERFORMANCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEPARATION TECHNIQUE;

ADSORPTION; AMMONIUM SULFATE; CHROMATOGRAPHY, LIQUID; COMPUTER SIMULATION; FINITE ELEMENT ANALYSIS; HYDROPHOBICITY; LIGANDS; LINEAR MODELS; MODELS, CHEMICAL; PROTEIN MULTIMERIZATION; PROTEINS; SEPHAROSE;

EID: 58149474802     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.12.002     Document Type: Article

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