Semi-automated relative quantification of cell culture contamination with mycoplasma by Photoshop-based image analysis on immunofluorescence preparations

Biologicals

Volumn 37, Issue 1, 2009, Pages 55-60

  Kumar, Ashok ^a   Yerneni, Lakshmana K ^a  

^a SAFDARJUNG HOSPITAL CAMPUS   (India)

Author keywords

Cell culture; Image analysis; Immunofluorescence; Mycoplasma contamination; Photoshop

Indexed keywords

DNA;

ANIMAL CELL; ARTICLE; AUTOMATION; BACTERIUM CONTAMINATION; CELL CULTURE; CONTROLLED STUDY; CYTOLOGY; DIAGNOSTIC ACCURACY; EMBRYO; FLUOROCHROME STAINING; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMMUNOFLUORESCENCE TEST; MOLECULAR IMAGING; MOUSE; MYCOPLASMA; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ANIMALS; AUTOMATIC DATA PROCESSING; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; EQUIPMENT CONTAMINATION; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MACACA MULATTA; MICE; MYCOPLASMA; SOFTWARE; VERO CELLS;

MYCOPLASMA;

EID: 58149343304     PISSN: 10451056     EISSN: 10958320     Source Type: Journal    
DOI: 10.1016/j.biologicals.2008.10.003     Document Type: Article

References (16)

1. Uphoff C.C., and Drexler H.G. Detection of mycoplasma contaminations. In: Helgason C.D., and Miller C.L. (Eds). Basic cell culture protocols. 3rd ed. Methods Mol Biol vol. 290 (2005) 13-23 Humana Press Inc., Totowa, NJ
2. Robinson I.B., Wichelhausen R.H., and Roizman B. Contamination of human cell cultures by pleuropneumonia-like organisms. Science 124 (1956) 1147-1148
3. McGarrity M.F., Vanaman V., and Sarama J. Cytogenetic effects of mycoplasmal infection of cell cultures: a review. In Vitro 20 (1984) 1-18
4. Hay R.J., Macy M.L., and Chen T.R. Mycoplasma infection of cultured cells. Nature 339 (1989) 487-488
5. Drexler H.G., and Uphoff C.C. Mycoplasma contamination of cell cultures. Incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39 (2002) 75-90
6. Blazek R., Schmitt K., Krafft U., and Hadding U. Fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody. J Immunol Methods 131 (1990) 203-212
7. Kamla V., Henrich B., and Hadding U. Species differentiation of mycoplasmas by EF-Tu specific monoclonal antibodies. J Immunol Methods 147 (1992) 73-81
8. Lehr H.-A., Loos C.M., van der T.P., and Gown A.M. Complete chromogen separation and analysis in double immunohistochemical stains using Photoshop-based image analysis. J Histochem Cytochem 47 (1999) 119-125
9. Chen T.R. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res 104 (1977) 255-262
10. Kumar A., Ali A., and Yerneni L.K. Tandem use of immunofluorescence and DNA staining assays to validate nested PCR detection of mycoplasma. In Vitro Cell Dev Biol Anim 44 (2008) 189-192
11. Hopert A., Uphoff C.C., Wirth M., Hauser H., and Drexler H.G. Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell cultures. J Immunol Methods 164 (1993) 91-100
12. Uphoff C.C., Gignac S.M., and Drexler H.G. Mycoplasma contamination in human leukemia cell lines: I. Comparison of various detection methods. J Immunol Methods 149 (1992) 43-53
13. Ishikawa Y., Kozakai T., Morita H., Saida K., Oka S., and Masuo Y. Rapid detection of mycoplasma contamination in cell cultures using SYBR Green-based real-time polymerase chain reaction. In Vitro Cell Dev Biol Anim 42 (2006) 63-69
14. Kumar A., Ali A., and Yerneni L.K. Effectiveness of a mycoplasma elimination reagent on a mycoplasma-contaminated hybridoma cell line. Hybridoma 26 (2007) 104-106
15. Uphoff C.C., and Drexler H.G. Detection of mycoplasma in leukemia-lymphoma cell lines using polymerase chain reaction. Leukemia 16 (2002) 289-293
16. Uphoff C.C., Brauer S., Grunicke D., Gignac S.M., MacLeod R.A.F., Quentmeier H., et al. Sensitivity and specificity of five different mycoplasma detection assays. Leukemia 6 (1992) 335-341