Tandem use of immunofluorescent and DNA staining assays to validate nested PCR detection of mycoplasma

In Vitro Cellular and Developmental Biology - Animal

Volumn 44, Issue 7, 2008, Pages 189-192

  Kumar, Ashok ^a   Ali, Arif ^b   Yerneni, Lakshmana K ^a  

^a SAFDARJUNG HOSPITAL   (India)

^b JAMIA MILLIA ISLAMIA CENTRAL UNIVERSITY   (India)

Author keywords

Cell culture contamination; Hoechst staining; Immunofluorescent assay; Mycoplasma; Nested PCR

Indexed keywords

DNA; MONOCLONAL ANTIBODY; BACTERIAL DNA;

ARTICLE; BACTERIUM CONTAMINATION; CYTOLOGY; GENE AMPLIFICATION; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; MYCOPLASMA; NONHUMAN; POLYMERASE CHAIN REACTION; BIOASSAY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; REPRODUCIBILITY;

BACTERIA (MICROORGANISMS); MYCOPLASMA;

BIOLOGICAL ASSAY; DNA, BACTERIAL; FALSE NEGATIVE REACTIONS; FALSE POSITIVE REACTIONS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MYCOPLASMA; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 55249089064     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-008-9081-5     Document Type: Article

References (16)

1. R Blazek K Schmitt U Krafft U Hadding 1990 Fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody J Immunol Methods 131 203 212
2. TR Chen 1977 In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain Exp Cell Res 104 255 262
3. HG Drexler CC Uphoff 2002 Mycoplasma contamination of cell cultures. Incidence, sources, effects, detection, elimination, prevention Cytotech 39 75 90
4. O Dussurget D Roulland-Dussoix 1994 Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera Appl Environ Microbial 60 953 959
5. R Harasawa H Mizusawa K Nozawa T Nakazawa K Asada I Kato 1993 Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions Res Microbiol 144 489 493
6. Y Ishikawa T Kozakai H Morita K Saida S Oka Y Masuo 2006 Rapid detection of mycoplasma contamination in cell cultures using SYBR Green-based real-time polymerase chain reaction In Vitro Cell Dev Biol Anim 42 63 69
7. V Kamla B Henrich U Hadding 1992 Species differentiation of mycoplasmas by EF-Tu specific monoclonal antibodies J Immunol Methods 147 73 81
8. Kong F.; James G.; Gordon S.; Zelynski A.; Gilbert G.L. Species-specific PCR for identification of common contaminant mollicutes in cell culture. Appl Environ Microbiol 67: 3195-3200; 2001.
9. Roblin III, Richard O., Hu M., Tang J.S., Lee S. Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers. United States Patent No. 5,693,467; 1997.
10. H Sung SH Kang YJ Bae JT Hong YB Chung C Lee S Song 2006 PCR-based detection of Mycoplasma species J Microbiol 44 1 42 49
11. J Tang M Hu S Lee R Robin 2000 A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture J Microbiol Methods 39 121 126
12. CC Uphoff HG Drexler 1999 Detection of mycoplasma contamination in cell cultures by PCR analysis Human Cell 12 229 236
13. CC Uphoff HG Drexler 2002 Detection of mycoplasma in leukemia-lymphoma cell lines using polymerase chain reaction Leukemia 16 289 293
14. Uphoff C.C.; Drexler H.G. Detection of mycoplasma contaminations. Helgason C.D., Miller C.L. (eds), In: Basic Cell Culture Protocols, third Edition, Methods in Mol Biol 290 Humana Press Inc, Totowa N J, 2005: pp 13-23.
15. CC Uphoff SM Gignac HG Drexler 1992 Mycoplasma contamination in human leukemia cell lines: I,, Comparison of various detection methods J Immunol Methods 149 43 53
16. H Wang F Kong P Jelfs G James GL Gilbert 2004 Simultaneous detection and identification of common cell culture contaminant and pathogenic Mollicutes strains by reverse line blot hybridization Appl Environm Microbiol 70 3 1483 1486