Purification of trypsinogen from bovine pancreas by combining aqueous two-phase partitioning and precipitation with charged flexible chain polymers

Separation and Purification Technology

Volumn 65, Issue 1, 2009, Pages 40-45

  Malpiedi, Luciana Pellegrini ^a   Romanini, Diana ^a   Picó, Guillermo A ^a   Nerli, Bibiana B ^a  

^a Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

Author keywords

Aqueous two phase systems; Pancreatic proteases; Partitioning; Trypsinogen

Indexed keywords

BIOMASS; ETHYLENE GLYCOL; GLYCOLS; NUCLEIC ACIDS; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; POLYETHYLENES; RECOVERY; RENEWABLE ENERGY RESOURCES; SODIUM; TWO PHASE FLOW;

AQUEOUS TWO-PHASE SYSTEMS; BIOMASS COMPOSITIONS; BOVINE PANCREASES; FLEXIBLE CHAINS; PANCREATIC PROTEASES; PARTITIONING; PHASE PARTITIONING; POLYETHYLENE IMINES; POSITIVELY CHARGED; PURIFICATION FACTORS; PURIFICATION PROCESSES; SEVERAL VARIABLES; SODIUM CITRATES; TIE LINES; TRYPSINOGEN; VOLUME RATIOS;

PURIFICATION;

BOVINAE;

EID: 58149302823     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2008.01.024     Document Type: Article

References (16)

1. Walsh K.A., Kauffman D.L., Sampath Kumar K.S.V., and Neurath H. On the structure and function of bovine trypsinogen and trypsin. Biochemistry 51 (1964) 301-308
2. Alves J., Chumpitaz L., Da Silva L., Franco T., and Mirelles A. Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems. J. Chromatogr. B 743 (2000) 235-239
3. Zafarani-Moattar M.T., and Hamidi A.A. Liquid-liquid equilibria of aqueous two-phase poly(ethylene glycol)-potassium citrate system. J. Chem. Eng. Data 48 (2003) 262-265
4. Tubio G., Pellegrini L., Nerli B.B., and Picó G.A. Liquid-liquid equilibria of aqueous two-phase systems containing poly(ethyleneglycols) of different molecular weight and sodium citrate. J. Chem. Eng. Data 51 (2006) 209-212
5. Burgess R.R. Use of polyethyleneimine in purification of DNA binding proteins. Methods in Enzymology: Protein-DNA Interactions (1991), Academic Press, London
6. Warburg O., and Christian W. Isolierung und Kristallisation des Garungsterments Enolase. Biochem. Z. 310 (1942) 384-421
7. Kay J., and Kassell B. The autoactivation of trypsinogen. J. Biol. Chem. 246 (1971) 6661-6665
8. Gildberg A., and Overbo K. Purification and characterization of pancreatic elastase from Atlantic cod (Gadus morhua). Comp. Biochem. Physiol. B 97 (1990) 775-782
9. In: Walter H., Brooks D., and Fisher D. (Eds). Partitioning in Aqueous Two-phase Systems. Theory, Methods, Uses, and Applications to Biotechnology (1985), Academic Press, Inc., Orlando
10. DeWalt B.W., Murphy J.C., Fox G.E., and Willson R.C. Compaction agent clarification of microbial lysates. Protein Expres. Purif. 28 (2003) 220-223
11. Dissing U., and Mattiasson B. Integrated removal of nucleic acids and recovery of LDH from homogenate of beef heart by affinity precipitation. Bioseparation 7 (1999) 221-229
12. Rito-Palomares M. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J. Chromatogr. B 807 (2004) 3-11
13. Rito-Palomares M., and Hernández M. Influence of system and process parameters on partitioning of cheese whey proteins in aqueous two-phase systems. J. Chromatogr. B 711 (1998) 81-90
14. Forciniti D., and Hall C.K. Protein partitioning at the isoelectric point: influence of polymer molecular weight and concentration and protein size. Biotechnol. Bioeng. 38 (1991) 986-994
15. Rito-Palomares M., and Lyddiatt A. Practical implementation of aqueous two-phase processes for protein recovery from yeast. J. Chem. Technol. Biotechnol. 75 (2000) 632-638
16. Albertsson P.A. Partition of Cell Particles and Macromolecules. 2nd ed. (1971), John Wiley and Sons, New York