Integrated removal of nucleic acids and recovery of LDH from homogenate of beef heart by affinity precipitation

Bioseparation

Volumn 7, Issue 4-5, 1998, Pages 221-229

  Dissing, Ulla ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Affinity precipitation; LDH; Nucleic acids; Poly(ethylene imine)

Indexed keywords

AFFINITY PRECIPITATION; CIBACRON BLUE F3GA; ENZYME PURIFICATION; LACTATE DEHYDROGENASE; NUCLEIC ACID;

AROMATIC POLYMERS; ENZYMES; NUCLEIC ACIDS; PH; PROTEINS; PURIFICATION;

AFFINITY PRECIPITATION; BEEF HEART; CIBACRON BLUE; LACTATE DEHYDROGENASE; POLYETHYLENE IMINE;

PRECIPITATION (CHEMICAL);

EID: 0032310891     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008003701018     Document Type: Article

References (17)

1. Ashwell G (1957) Colorimetric analysis of sugars. In: Colowick SP and Kaplan NO (eds), Methods in Enzymology, Vol. III pp. 73-105. Academic Press, New York.
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248-254.
3. Clark KM and Glatz, CE (1987) Polymer dosage considerations in polyelectrolyte precipitation of proteins. Biotech. Progr. 4: 241-247.
4. Dissing U and Mattiasson B (1996) Polyelectrolyte complexes as vehicles for affinity precipitation of proteins. J. Biotechnol. 52: 241-247.
5. Fujii M and Taniguchi M (1991) Application of reversibly soluble polymers in bioprocessing. Trends Biotechnol. 9: 191-196.
6. Galaev IYu and Mattiasson B (1993) Thermoreactive water soluble polymers, nonionic surfactants, and hydrogels as reagents in biotechnology. Enzyme Microb. Technol. 15: 354-366.
7. Izumrudov VA, Galaev IYu and Mattiasson B (1999) Polycomplexes - potential for bioseparation. Bioseparation 7: 207-220.
8. Kabanov VA (1994) Basic properties of soluble interpolyelectrolyte complexes applied to bioengineering and cell transformations. In: Dubin PL (ed.), Macromolecular Complexes in Chemistry and Biology, Ch. 10 (pp. 151-174). Springer Verlag, Berlin-Heidelberg.
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
10. Milburn P, Bonnerjea J, Hoare M and Dunnill P (1990) Selective flocculation of nucleic acids, lipids, and colloidal particles from a yeast cell homogenate by polyethyleneimine, and its scale-up. Enzyme Microb. Technol. 12: 527-532.
11. Monji N and Hoffman AS (1987) A novel immunoassay system and bioseparation process based on thermal phase separating polymers. Appl. Biochem. Biotechnol. 14: 107-120.
12. Morris JE, Hoffman AS and Fisher RR (1993) Affinity precipitation of proteins by polyligands. Biotechnol. Bioeng. 41: 991-997.
13. Schneider WC (1957) Determination of nucleic acids in tissues by pentose analysis. In: Colowick SP and Kaplan NO (eds), Methods in Enzymology, Vol. III. (pp. 680-684). Academic Press, New York.
14. Senstad C and Mattiasson M (1989) Affinity-precipitation using chitosan as ligand carrier. Biotechnol. Bioeng. 33: 216-220.
15. Strege MA, Dubin PL, West JS and Flinta CD (1990) Complexation between poly(dimethyldiallylammonium chloride) and globular proteins. In: Hamel J-FP, Hunter JB and Sikdar SK (eds), Downstream Processing and Bioseparation (pp. 158-169). ACS symposium series, Washington, DC.
16. Thompson ST and Stellwagen E (1976) Binding of Cibacron blue F3GA to proteins containing the dinucleotide fold. Proc. Natl. Acad. Sci. USA 73: 361-365.
17. Worthington Enzyme Manual (1977) Tn: Decker LA (ed.) (pp. 7-10). Freehold, New Jersey, USA.