Functional and immunological characterization of a natural polymorphic variant of a heat-labile toxin (LT-I) produced by enterotoxigenic Escherichia coli (ETEC): RESEARCH ARTICLE

FEMS Immunology and Medical Microbiology

Volumn 55, Issue 1, 2009, Pages 93-99

  Lasaro, Melissa A ^a,b   Mathias Santos, Camila ^a   Rodrigues, Juliana F ^a   Ferreira, Luís C S ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

ETEC; Heat labile toxin; LT; Mucosal adjuvant

Indexed keywords

BACTERIAL TOXIN; CYCLIC AMP; HEAT LABILE TOXIN 1; HEAT LABILE TOXIN 2; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; COMPARATIVE STUDY; ENTEROTOXIGENIC ESCHERICHIA COLI; FEMALE; IMMUNIZATION; IMMUNOGENICITY; IMMUNOLOGY; IMMUNOMODULATION; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ADMINISTRATION, CUTANEOUS; ADMINISTRATION, ORAL; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, BACTERIAL; ANTITOXINS; BACTERIAL TOXINS; CELL LINE; ENTEROTOXIGENIC ESCHERICHIA COLI; ENTEROTOXINS; ESCHERICHIA COLI PROTEINS; MICE; MUTATION, MISSENSE; NEUTRALIZATION TESTS; POLYMORPHISM, GENETIC;

EID: 58149293359     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2008.00506.x     Document Type: Article

References (26)

1. Clements JD, Hartzog NM Lyon FL (1988) Adjuvant activity of Escherichia coli heat-labile enterotoxin and effect on the induction of oral tolerance in mice to unrelated protein antigens. Vaccine 6 : 269 277.
2. Dallas WS (1983) Conformity between heat-labile toxin genes from human and porcine enterotoxigenic Escherichia coli. Infect Immun 40 : 647 652.
3. Donta ST, Moon HW Whipp SC (1974) Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science 183 : 334 336.
4. Elson CO Ealding W (1984) Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin. J Immunol 132 : 2736 2741.
5. Evans DG, Silver RP, Evans Jr. DJ, Chase DG Gorbach SL (1975) Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infect Immun 12 : 656 667.
6. Faria AM Weiner HL (1999) Oral tolerance: mechanisms and therapeutic applications. Adv Immunol 73 : 153 264.
7. Fraser SA, de Haan L, Hearn AR et al 2003) Mutant Escherichia coli heat-labile toxin B subunit that separates toxoid-mediated signaling and immunomodulatory action from trafficking and delivery functions. Infect Immun 71 : 1527 1537.
8. Glenn GM, Scharton-Kersten T Alving CR (1999) Advances in vaccine delivery: transcutaneous immunisation. Expert Opin Inv Drugs 8 : 797 805.
9. Gothefors L, Ahren C, Stoll B, Barua DK, Orskov F, Salek MA Svennerholm AM (1985) Presence of colonization factor antigens on fresh isolates of fecal Escherichia coli: a prospective study. J Infect Dis 152 : 1128 1133.
10. Guillobel HC, Carinhanha JI, Cardenas L, Clements JD, de Almeida DF Ferreira LC (2000) Adjuvant activity of a nontoxic mutant of Escherichia coli heat-labile enterotoxin on systemic and mucosal immune responses elicited against a heterologous antigen carried by a live Salmonella enterica serovar Typhimurium vaccine strain. Infect Immun 68 : 4349 4353.
11. Holmgren J, Czerkinsky C, Eriksson K Mharandi A (2003) Mucosal immunisation and adjuvants: a brief overview of recent advances and challenges. Vaccine 21 (suppl 2) : S89 S95.
12. Imamura S, Kido N, Kato M, Kawase H, Miyama A Tsuji T (1997) A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA. FEMS Microbiol Lett 146 : 241 245.
13. Lasaro MA, Rodrigues JF, Mathias-Santos C et al 2006) Production and release of heat-labile toxin by wild-type human-derived enterotoxigenic Escherichia coli. FEMS Immunol Med Microbiol 48 : 123 131.
14. Lasaro MA, Rodrigues JF, Mathias-Santos C, Guth BE, Balan A, Sbrogio-Almeida ME Ferreira LC (2008) Genetic diversity of heat-labile toxin expressed by enterotoxigenic Escherichia coli strains isolated from humans. J Bacteriol 190 : 2400 2410.
15. Leong J, Vinal AC Dallas WS (1985) Nucleotide sequence comparison between heat-labile toxin B-subunit cistrons from Escherichia coli of human and porcine origin. Infect Immun 48 : 73 77.
16. Pizza M, Domenighini M, Hol W et al 1994) Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis. Mol Microbiol 14 : 51 60.
17. Qadri F, Khan AI, Faruque AS et al 2005) Enterotoxigenic Escherichia coli and Vibrio cholerae diarrhea, Bangladesh, 2004. Emerg Infect Dis 11 : 1104 1107.
18. Rosa DS, Tzelepis F, Cunha MG, Soares IS Rodrigues MM (2004) The pan HLA DR-binding epitope improves adjuvant-assisted immunization with a recombinant protein containing a malaria vaccine candidate. Immunol Lett 92 : 259 268.
19. Sambrook J, Fritsch EF Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
20. Schweizer HP (2001) Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads. Curr Opin Biotech 12 : 439 445.
21. Simmons CP, Ghaem-Magami M, Petrovska L, Lopes L, Chain BM, Williams NA Dougan G (2001) Immunomodulation using bacterial enterotoxins. Scand J Immunol 53 : 218 226.
22. Spangler BD (1992) Structure and function of cholera toxin and the related Escherichia coli heat-labile enterotoxin. Microbiol Rev 56 : 622 647.
23. Streatfield SJ, Sandkvist M, Sixma TK, Bagdasarian M, Hol WG Hirst TR (1992) Intermolecular interactions between the A and B subunits of heat-labile enterotoxin from Escherichia coli promote holotoxin assembly and stability in vivo. P Natl Acad Sci USA 89 : 12140 12144.
24. Tsuji T, Taga S, Honda T, Takeda Y Miwatani T (1982) Molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli. Infect Immun 38 : 444 448.
25. Walker R Clements JD (1993) Use of heat-labile toxin of enterotoxigenic Escherichia coli to facilitate mucosal immunization. Vaccine Research 2 : 1 10.
26. Yamamoto T, Tamura T Yokota T (1984) Primary structure of heat-labile enterotoxin produced by Escherichia coli pathogenic for humans. J Biol Chem 259 : 5037 5044.