Effects of metoclopramide on mRNA levels of 5α-reductase isozymes in rat brain

NeuroReport

Volumn 20, Issue 1, 2009, Pages 93-96

  Sánchez, Pilar ^a   Torres, Jesús M ^a,b   Vílchez, Pablo ^a   Del Moral, Raimundo G ^a   Ortega, Esperanza ^a,b,c  

^a HOSPITAL UNIVERSITARIO SAN CECILIO   (Spain)

^b UNIVERSITY OF GRANADA   (Spain)

^c UNIVERSITY OF GRANADA   (Spain)

Author keywords

5 reductase isozymes; Adult rat; Hyperprolactinemia; Metoclopramide; mRNA levels; Prefrontal cortex

Indexed keywords

5 ALPHA REDUCTASE; ISOENZYME; MESSENGER RNA; METOCLOPRAMIDE; PROLACTIN; SULPIRIDE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG EFFECT; ENZYME REGULATION; FEMALE; HORMONE BLOOD LEVEL; HORMONE RELEASE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PREFRONTAL CORTEX; PRIORITY JOURNAL; RAT;

ANIMALS; DOPAMINE ANTAGONISTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; INJECTIONS, INTRAPERITONEAL; ISOENZYMES; LINEAR MODELS; MALE; METOCLOPRAMIDE; PREFRONTAL CORTEX; PROLACTIN; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 58149267782     PISSN: 09594965     EISSN: None     Source Type: Journal    
DOI: 10.1097/WNR.0b013e32831ac999     Document Type: Article

References (25)

1. Torner L, Maloumby R, Nava G, Aranda J, Clapp C, Neumann ID. In vivo release and gene upregulation of brain prolactin in response to physiological stimuli. Eur J Neurosci 2004; 19:1601-1608.
2. Bridges RS, DiBiase R, Loundes DD, Doherty PC. Prolactin stimulation of maternal behavior in female rats. Science 1985; 227:782-784.
3. Bridges RS, Ronsheim PM. Prolactin (PRL) regulation of maternal behavior in rats: bromocriptine treatment delays and PRL promotes the rapid onset of behavior. Endocrinology 1990; 126:837-848.
4. Purdy RH, Morrow AL, Moore PH Jr, Paul SM. Stress-induced elevations of gamma-aminobutyric acid type A receptor-active steroids in the rat brain. Proc Natl Acad Sci U S A 1991; 88:4553-4557.
5. Majewska MD. Neurosteroids: endogenous bimodal modulators of the GABAA receptor. Mechanism of action and physiological significance. Prog Neurobiol 1992; 38:379-395.
6. Mellon SH, Griffin LD, Compagnone NA. Biosynthesis and action of neurosteroids. Brain Res Brain Rev 2001; 37:3-12.
7. Sánchez P, Torres JM, Vílchez P, Del Moral RG, Ortega E. Effects of sulpiride on prolactin and mRNA levels of steroid 5 alpha-reductase isozymes in adult rat brain. Neurochem Res 2008; 33:820-825.
8. Debeljuk L, Rozados R, Daskal H, Velez V, Mancini AM. Acute and chronic effects of sulpiride on serum prolactin and gonadotropin levels in castrated male rats (38581). Proc Soc Biol Med 1975; 148:550-552.
9. Słuczanowska-Głabowska S, Laszczyn̄ska M, Głabowski W, Wylot M. Morphology of the epithelial cells and expression of androgen receptor in rat prostate dorsal lobe in experimental hyperprolactinemia. Folia Histochem Cytobiol 2006; 44:25-30.
10. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 2nd ed. New York: Academic Press; 1986.
11. Torres JM, Ortega E. Quantitation of mRNA levels of steroid 5alpha-reductase isozymes: a novel method that combines quantitative RT-PCR and capillary electrophoresis. Int J Biochem Cell B 2004; 36:78-88.
12. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156-159.
13. Yamanaka H, Kirdani RY, Saroff J, Murphy GP, Sandberg AA. Effects of testosterone and prolactin on rat prostatic weight, 5alpha-reductase, and arginase. Am J Physiol 1975; 229:1102-1109.
14. Uzunova V, Sheline Y, Davis JM, Rasmusson A, Uzunov DP, Costa E, et al. Increase in the cerebrospinal fluid content of neurosteroids in patients with unipolar major depression who are receiving fluoxetine or fluvoxamine. Proc Natl Acad Sci 1998; 95:3239-3244.
15. Dong E, Matsumoto K, Uzunova V, Sugaya I, Takahata H, Nomura H, et al. Brain 5α-dihydroprogesterone and allopregnanolone synthesis in a mouse model of protracted social isolation. Proc Natl Acad Sci U S A 2001; 98:2849-2854.
16. Guidotti A, Dong E, Matsumoto K, Pinna G, Rasmusson AM, Costa E. The socially-isolated mouse: a model to study the putative role of allopregnanolone and 5α-dihydroprogesterone in psychiatric disorders. Brain Res Rev 2001; 37:110-115.
17. Van Broekhoven F, Verkes RJ. Neurosteroids in depression: a review. Psychopharmacology 2003; 165:97-110.
18. Marx CE, Stevens RD, Shampine U, Uzunova V, Trost WT, Butterfield MI, et al. Neuroactive steroids are altered in schizophrenia and bipolar disorder: relevance to pathophysiology and therapeutics. Neuropsychopharmacology 2006; 31:1249-1263.
19. Torres JM, Ortega E. Differential regulation of steroid 5alpha-Reductase isozymes expression by androgens in the adult rat brain. FASEB J 2003; 17:1428-1433.
20. Torres JM, Ruiz E, Ortega E. Effects of CRH and ACTH administration on plasma and brain neurosteroid levels. Neurochem Res 2001; 26:555-558.
21. Torres JM, Ortega E. Steroid 5α-Reductase isozymes in the adult female rat brain: central role of dihydrotestosterone. J Mol Endocrinol 2006; 36:239-245.
22. Negri-Cesi P, Poletti A, Celotti F. Metabolism of steroids in the brain: a new insight into the role of 5alpha-Reductase and Aromatase in brain differentiation and functions. J Steroid Biochem Mol Biol 1996; 58:455-466.
23. Sánchez P, Torres JM, Ortega E. Effects of dihydrotestosterone on brain mRNA levels of steroid 5alpha-Reductase isozymes in early postnatal He of rat. Neurochem Res 2005; 30:577-581.
24. Poletti A, Coscarella A, Negri-Cesi P, Colciago A, Celotti F, Martini L. 5 alpha Reductase isozymes in the central nervous system. Steroids 1998; 63:246-251.
25. Torres JM, Ruiz E, Ortega E. Development of a quantitative RT-PCR method to study 5alpha-Reductase mRNA isozymes in rat prostate in different androgen status. Prostate 2003; 56:74-79.