Continuous spectrofluorometric and spectrophotometric assays for NADPH-cytochrome P450 reductase activity using 5-cyano-2,3-ditolyl tetrazolium chloride

Biotechnology Letters

Volumn 31, Issue 2, 2009, Pages 271-275

  Kim, Dong Hyun ^a   Yim, Sung Kun ^a   Kim, Keon Hee ^a   Ahn, Taeho ^a   Yun, Chul Ho ^a  

^a Chonnam National University   (South Korea)

Author keywords

5 cyano 2,3 ditolyl tetrazolium chloride; Absorbance; Continuous assay; Fluorescence; NADPH cytochrome P450 reductase; Reduction activity

Indexed keywords

ASSAYS; ATOMS; CATALYSTS; CHLORINE COMPOUNDS; ENZYMES; FLUORESCENCE; MAMMALS; PROTEINS;

5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE; ABSORBANCE; CONTINUOUS ASSAY; NADPH-CYTOCHROME P450 REDUCTASE; REDUCTION ACTIVITY;

TEMPERATURE CONTROL;

5 CYANO 2,3 DITOLYLTETRAZOLIUM; 5-CYANO-2,3-DITOLYLTETRAZOLIUM; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; TETRAZOLIUM;

ARTICLE; CHEMISTRY; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYME STABILITY; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY;

ENZYME ACTIVATION; ENZYME STABILITY; NADPH-FERRIHEMOPROTEIN REDUCTASE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; TETRAZOLIUM SALTS;

MAMMALIA;

EID: 58149197763     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9865-4     Document Type: Article

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