Reduction of a tetrazolium salt, CTC, by intact HepG2 human hepatoma cells: subcellular localisation of reducing systems

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1451, Issue 1, 1999, Pages 73-81

  Bernas, Tytus ^a   Dobrucki, Jurek ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

CTC; Plasma membrane; Reductase; Tetrazolium salt

Indexed keywords

ARTICLE; CELL STRAIN HEPG2; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; HUMAN; HUMAN CELL; IN VITRO STUDY; NITROBLUE TETRAZOLIUM TEST; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; REACTION ANALYSIS;

ANIMALIA; BACTERIA (MICROORGANISMS);

EID: 0032865951     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4889(99)00071-3     Document Type: Article

References (38)

1. Satoh T., Isobe H., Ayukawa K., Sakai H., Nawata H. The effects of pravastatin, an HMG-CoA reductase inhibitor, on cell viability and DNA production of rat hepatocytes. Life Sci. 59:1996;1103-1108.
2. Shearman M.S. Cellular MTT reduction distinguishes the mechanism of action of beta-amyloid from that of tachykinin receptor peptides. Neuropeptides. 30:1996;125-132.
3. Shearman M.S., Hawtin S.R., Tailor V.J. The intracellular component of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction is specifically inhibited by beta-amyloid peptides. J. Neurochem. 65:1995;218-227.
4. Marshall N.J., Goodwin C.J., Holt S.J. A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth Regul. 5:1995;69-84.
5. Goodwin C.J., Holt S.J., Riley P.A., Downes S., Marshall N.J. Growth hormone-responsive DT-diaphorase-mediated bioreduction of tetrazolium salts. Biochem. Biophys. Res. Commun. 226:1996;935-941.
6. Liu Y., Schubert D. Cytotoxic amyloid peptides inhibit cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction by enhancing MTT formazan exocytosis. J. Neurochem. 69:1997;2285-2293.
7. Prohaska H.J., Santamaria A.B. Quinone reductase from cells cultured in microliter wells: a screening assay for anticarcinogenic enzyme inducers. Anal. Biochem. 169:1988;328-336.
8. Carpenter Deyo L., Reed D.J. Involvement of calcium and iron in Quin 2 toxicity to isolated hepatocytes. J. Pharmacol. Exp. Ther. 258:1991;747-752.
9. Gorog P., Kovacs I.B. Lipid peroxidation by activated platelets: a possible link between thrombosis and atherogenesis. Atherosclerosis. 115:1995;121-128.
10. Benau D.A., Schumacher W.G., McGuire E.J., Fitzpatrick McElligott S., Storey B.T., Roth S. Light microscopic localization of glycosyltransferase activities in cells and tissues. J. Histochem. Cytochem. 38:1990;23-30.
11. Kooij A., Frederiks W.M., Gossrau R., Van Noorden C.J. Localization of xanthine oxidoreductase activity using the tissue protectant polyvinyl alcohol and final electron acceptor Tetranitro BT. J. Histochem. Cytochem. 39:1990;87-93.
12. Faber Zuschratter H., Seidenbecher T., Reymann K., Wolf G. Ultrastructural distribution of NADPH-diaphorase in the normal hippocampus and after long-term potentiation. J. Neural Transm. Gen. Sect. 103:1996;807-817.
13. Stellmach J. Fluorescent redox dyes. 1. Production of fluorescent formazan by unstimulated and phorbol ester- or digitonin-stimulated Erlich ascites tumor cells. Histochemistry. 80:1984;137-143.
14. Stellmach J., Severin E. A fluorescent redox dye. Influence of several substrates and electron carriers on the tetrazolium salt-formazan reaction of Ehrlich ascites tumour cells. Histochem. J. 19:1987;21-26.
15. van Noorden C., Dolbeare F., Aten J. Flow cytofluorimetric analysis of enzyme reactions based on quenching of fluorescence by final reaction product: detection of glucose-6-phosphate dehydrogenase deficiency in human erythrocytes. J. Histochem. Cytochem. 37:1989;1313-1318.
16. Mire-Sluis A.R., Thorpe R. Laboratory protocols for quantitation of cytokines by bioassay using cytokine responsive cell lines. J. Immunol. Methods. 211:1998;199-210.
17. Alley M.C., Scudiero D.A., Monks A.E. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48:1988;589-601.
18. Thayer W.S. Superoxide-dependent and superoxide-independent pathways for reduction of nitroblue tetrazolium in isolated rat cardiac myocytes. Arch. Biochem. Biophys. 276:1990;139-145.
19. Berridge M.V., Tan A.S. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 303:1993;474-482.
20. J.B. Pawley (Ed.), Handbook of Biological Confocal Microscopy, Plenum Press, New York, 1995, pp. 167-173.
21. van Noorden C., Tas J. The role of exogenous electron carriers in NAD(P)-dependent dehydrogenase cytochemistry studied in vitro and with model system of polyacrylamide films. J. Histochem. Cytochem. 30:1982;12-20.
22. London N.J., Contractor H., Lake S.P., Aucott G.C., Bell P.R., James R.F. A microfluorometric viability assay for isolated human and rat islets of Langerhans. Diabetes Res. 12:1989;141-149.
23. Herman B., Gores G.J., Nieminen A.L., Kawanishi T., Harman A., Lemasters J.J. Calcium and pH in anoxic and toxic injury. Crit. Rev. Toxicol. 21:1990;127-148.
24. Rodriguez G.G., Phipps D., Ishiguro K., Ridgway H.F. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1992;1801-1808.
25. Schaule G., Flemming H.C., Ridgway H.F. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59:1993;3850-3857.
26. Altman F.P. Tetrazolium salts and formazans. Prog. Histochem. Cytochem. 9:1976;1-56.
27. Kugler P., Wrobel K.H. Meldola Blue: a new electron carrier for the histochemical demonstration of dehydrogenases (SDH, LDH, G-6-PDH). Histochemistry. 59:1978;97-109.
28. Heo D.S., Park J.G., Hata K., Day R., Herberman R.B., Whiteside T.L. Evaluation of tetrazolium-based semiautomatic colorimetric assay for measurement of human antitumor cytotoxicity. Cancer Res. 50:1990;3681-3690.
29. Crane F.L., Sun I.L., Clark M.G., Grebing C., Low H. Transplasma-membrane redox systems in growth and development. Biochim. Biophys. Acta. 811:1985;233-264.
30. Morre D.J., Brightman A.O. NADH oxidase of plasma membranes. J. Bioenerg. Biomembr. 23:1991;469-489.
31. Meier B., Cross A.R., Hancock J.T., Kaup F.J., Jones O.T. Identification of a superoxide-generating NADPH oxidase system in human fibroblasts. Biochem. J. 275:1991;241-245.
32. Mukherjee S.P., Lynn W.S. Reduced nicothiamide adenine dinucleotide phosphate oxidase and its activation by insulin. Arch. Biochem. Biophys. 184:1977;69-76.
33. Mizukami Y., Matsubara F., Matsukawa S. Cytochemical localization of peroxidase and hydrogen-peroxide-producing NAD(P)H-oxidase in thyroid follicular cells of propylthiouracil-treated rats. Histochemistry. 82:(3):1985;263-268.
34. Mizukami Y., Matsubara F., Matsukawa S., Izumi R. Cytochemical localization of glutaraldehyde-resistant NAD(P)H-oxidase in rat hepatocytes. Histochemistry. 79:(2):1983;259-267.
35. Sun I.L., Navas P., Crane F.L., Morre D.J., Low H. NADH diferric transferrin reductase in liver plasma membrane. J. Biol. Chem. 262:1987;15915-15921.
36. Bernas T., Dobrucki J. Extracellular reduction of Cat1 free radical by transformed human hepatocytes. Biosci. Rep. 18:(6):1999;341-350.
37. B. Halliwell, J.M.C. Gutteridge (Eds.), Free Radicals in Biology and Medicine, Oxford University Press, Oxford, 1985.
38. Bruno M., Brightman A.O., Lawrence J., Werderitsh D., Morre D.M., Morre D.J. Stimulation of NADH oxidase activity from rat liver plasma membranes by growth factors and hormones is decreased or absent with hepatoma plasma membranes. Biochem. J. 284:1992;625-628.