STR profiles from DNA samples with "undetected" or low quantifiler™ results

Journal of Forensic Sciences

Volumn 54, Issue 1, 2009, Pages 103-107

  Cupples, Catherine M ^b   Champagne, Jarrod R ^c   Lewis, Kristen E ^d   Cruz, Tracey Dawson ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b LABORATORY CORPORATION OF AMERICA   (United States)

^c Fairfax Identity Laboratories   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

Author keywords

DNA quantitation; DNA typing; Forensic science; Low level DNA; Quantifiler ; Quantitative polymerase chain reaction

Indexed keywords

DNA;

ADULT; ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DILUTION; DNA EXTRACTION; DNA FINGERPRINTING; DNA SCREENING; FEMALE; GENE AMPLIFICATION; GENE LOCUS; HUMAN; HUMAN EXPERIMENT; MALE; ORAL BIOPSY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHORT TANDEM REPEAT;

ADULT; DNA; DNA FINGERPRINTING; ELECTROPHORESIS, CAPILLARY; FEMALE; HUMANS; MALE; POLYMERASE CHAIN REACTION; TANDEM REPEAT SEQUENCES; YOUNG ADULT;

EID: 58149186843     PISSN: 00221198     EISSN: 15564029     Source Type: Journal    
DOI: 10.1111/j.1556-4029.2008.00914.x     Document Type: Article

References (17)

1. Fregeau CJ, Fourney RM. DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification. Biotechniques 1993 15 (1 100 19.
2. Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 2001 46 (3 647 60.
3. Walsh PS, Varlaro J, Reynolds R. A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acids Res 1992 20 (19 5061 5.
4. Mandrekar MN, Erickson AM, Kopp K, Krenke BE, Mandrekar PV, Nelson R, et al. Development of a human DNA quantitation system. Croat Med J 2001 42 (3 336 9.
5. Green RL, Roinestad IC, Boland C, Hennessy LK. Developmental validation of the Quantifiler™ real-time PCR kits for the quantification of human nuclear DNA samples. J Forensic Sci 2005 50 (4 809 25.
6. Kline MC, Duewer DL, Redman JW, Butler JM. Results from the NIST 2004 DNA quantitation study. J Forensic Sci 2005 50 (3 570 8.
7. Butler JM. Forensic DNA typing: biology and technology behind STR markers, 1st edition. San Diego, CA : Academic Press, 2001.
8. Timken MD, Swango KL, Orrego C, Buoncristiani MR. A duplex real-time qPCR assay for the quantification of human nuclear and mitochondrial DNA in forensic samples: implications for quantifying DNA in degraded samples. J Forensic Sci 2005 50 (5 1044 60.
9. Hayn S, Wallace MM, Prinz M, Shaler RC. Evaluation of an automated liquid hybridization method for DNA quantitation. J Forensic Sci 2004 49 (1 87 91.
10. Lewis KE, Sykes KLV, Greenspoon SA, Coy KL, Cupples CM, Rodier DN, et al. Comparative analysis of human-specific DNA quantitation techniques. Proceedings of the 58th Annual Meeting of the American Academy of Forensic Sciences, 2006 Feb. 20-25. Seattle, WA; Colorado Springs, CO : American Academy of Forensic Sciences, 2006.
11. Duewer DL, Kline MC, Redman JW, Newall PJ, Reeder DJ. NIST mixed stain studies #1 and #2: interlaboratory comparison of DNA quantitation practice and short tandem repeat multiplex performance with multiple source samples. J Forensic Sci 2001 46 (5 1199 210.
12. Kline MC, Duewer DL, Redman JW, Butler JM. NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity. Anal Chem 2003 75 (10 2463 9.
13. Coy KL, Lewis KE, Fulmer A, Hudson A, Dawson Cruz T. Obtaining typable DNA from bloodstains that serologically test negative. J Forensic Ident 2005 55 (5 633 42.
14. Tanaka M, Yoshimoto T, Nozawa H, Ohtaki H, Kato Y, Sato K, et al. Usefulness of a toothbrush as a source of evidential DNA for typing. J Forensic Sci 2000 45 (3 674 6.
15. Greenspoon SA, Scarpetta MA, Drayton ML, Turk SA. QIAamp spin columns as a method of DNA isolation for forensic casework. J Forensic Sci 1998 43 (5 1024 30.
16. Applied Biosystems. AmpFlSTR® Profiler Plus™ PCR Amplification Kit: user's manual, part number 4303501C. Foster City, CA : Applied Biosystems, 2000.
17. Applied Biosystems. GeneMapper® ID software versions 3.1 and 3.2 human identification analysis tutorial, part number 4357520 Rev. A. Foster City, CA : Applied Biosystems, 2004.