High-resolution imaging of Redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein

Science Signaling

Volumn 1, Issue 43, 2008, Pages

  Maulucci, Giuseppe ^a   Labate, Valentina ^a   Mele, Marina ^a   Panieri, Emiliano ^a   Arcovito, Giuseppe ^a   Galeotti, Tommaso ^a   Østergaard, Henrik ^b   Winther, Jakob R ^c   De Spirito, Marco ^a   Pani, Giovambattista ^a  

^a CATHOLIC UNIVERSITY   (Italy)

^b NOVO NORDISK A S   (Denmark)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DIAGNOSTIC AGENT; PHOTOPROTEIN;

ANIMAL; ARTICLE; CELL; CONFOCAL MICROSCOPY; CYTOLOGY; HUMAN; OXIDATION REDUCTION REACTION; SIGNAL TRANSDUCTION; TECHNIQUE;

ANIMALS; CELLS; HUMANS; LUMINESCENT PROTEINS; METHODS; MICROSCOPY, CONFOCAL; OXIDATION-REDUCTION; SIGNAL TRANSDUCTION;

EID: 58149180823     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.143pl3     Document Type: Article

References (13)

1. T. Finkel, Oxidant signals and oxidative stress. Curr. Opin. Cell Biol. 15, 247-254 (2003).
2. G. Pani, B. Bedogni, R. Colavitti, R. Anzevino, S. Borrello, T. Galeotti, Cell compartmentalization in redox signaling. IUBMB Life 52, 7-16 (2001).
3. S. G. Rhee, S. W. Kang, W. Jeong, T. S. Chang, K. S. Yang, H. A. Woo, Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Curr. Opin. Cell Biol. 17, 183-189 (2005).
4. H. Liu, R. Colavitti, I. I. Rovira, T. Finkel, Redox-dependent transcriptional regulation. Circ. Res. 97, 967-974 (2005).
5. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum, New York, ed. 2, 1997).
6. Z. Gryczynski, I. Gryczynski, J. R. Lakowicz, Fluorescence sensing methods. Methods Enzymol. 360, 44-75 (2003).
7. J. Zhang, R. E. Campbell, A. Y. Ting, R. Y. Tsien, Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002).
8. H. Østergaard, A. Henriksen, F. G. Hansen, J. R. Winther, Shedding light on disulfide bond formation: Engineering a redox switch in green fluorescent protein. EMBO J. 20, 5853-5862 (2001).
9. O. Björnberg, H. Ostergaard, J. R. Winther, Measuring intracellular redox conditions using GFP-based sensors. Antioxid. Redox Signal. 8, 354-361 (2006).
10. C. T. Dooley, T. M. Dore, G. T. Hanson, W. C. Jackson, S. J. Remington, R. Y. Tsien, Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J. Biol. Chem. 279, 22284-22293 (2004).
11. G. T. Hanson, R. Aggeler, D. Oglesbee, M. Cannon, R. A. Capaldi, R. Y. Tsien, S. J. Remington, Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. J. Biol. Chem. 279, 13044-13053 (2004).
12. M. Gutscher, A. L. Pauleau, L. Marty, T. Brach, G. H. Wabnitz, Y. Samstag, A. J. Meyer, T. P. Dick, Real-time imaging of the intracellular glutathione redox potential. Nat. Methods 5, 553-559 (2008).
13. O. R. Koch, S. Fusco, S. C. Ranieri, G. Maulucci, P. Palozza, L. M. Larocca, A. A. M. Cravero, S. M. Farre', M. De Spirito, T. Galeotti, G. Pani, Role of the life span determinant P66shcA in ethanol-induced liver damage. Lab. Invest. 88, 750-760 (2008).