Real-time PCR and subsequent pyrosequencing for screening of penA mosaic alleles and prediction of reduced susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae

APMIS

Volumn 116, Issue 11, 2008, Pages 1004-1008

  Unemo, Magnus ^a   Olcén, Per ^a   Fredlund, Hans ^a   Thulin, Sara ^a  

^a ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

Author keywords

CefI; Cephalosporin resistance; Neisseria gonorrhoeae; penA; Pyrosequencing; Real time PCR

Indexed keywords

AZITHROMYCIN; CEFIXIME; CEFTRIAXONE; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC SENSITIVITY; GENE FREQUENCY; GENE IDENTIFICATION; GENE MUTATION; GENETIC CODE; GONORRHEA; IN VITRO STUDY; LETTER; NEISSERIA GONORRHOEAE; NONHUMAN; PENICILLIN RESISTANCE; PREVALENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PYROSEQUENCING; REAL TIME POLYMERASE CHAIN REACTION;

ALLELES; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; CEPHALOSPORINS; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; GONORRHEA; HUMANS; MOLECULAR SEQUENCE DATA; NEISSERIA GONORRHOEAE; PENICILLIN-BINDING PROTEINS; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 58149145216     PISSN: 09034641     EISSN: 16000463     Source Type: Journal    
DOI: 10.1111/j.1600-0463.2008.01062.x     Document Type: Letter

References (18)

1. CDC. Sexually Transmitted Disease Surveillance 2004 Supplement: Gonococcal Isolate Surveillance Project (GISP) Annual Report - 2004. Atlanta Georgia : U.S. Department of Health and Human Services, 2005.
2. Deguchi T, Yasuda M, Yokoi S, Ishida K, Ito M, Ishihara S, et al. Treatment of uncomplicated gonococcal urethritis by double-dosing of 200 mg cefixime at a 6-h interval. J Infect Chemother 2003 9 : 35 9.
3. Dillon JA, Ruben M, Li H, Borthagaray G, Marquez C, Fiorito S, et al. Challenges in the control of gonorrhoea in South America and the Caribbean: monitoring the development of resistance to antibiotics. Sex Transm Dis 2006 33 : 87 95.
4. Martin IM, Hoffmann S, Ison CA. European Surveillance of Sexually Transmitted Infections (ESSTI). The first combined antimicrobial susceptibility data for Neisseria gonorrhoeae in Western Europe. J Antimicrob Chemother 2006 58 : 587 93.
5. The WHO Western Pacific Gonococcal Antimicrobial Surveillance Programme. Surveillance of antibiotic resistance in Neisseria gonorrhoeae in the WHO Western Pacific Region, 2004. Commun Dis Intell 2006 30 : 129 32.
6. Ameyama S, Onodera S, Takahata M, Minami S, Maki N, Endo K, et al. Mosaic-like structure of penicillin-binding protein 2 gene (penA) in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to cefixime. Antimicrob Agents Chemother 2002 46 : 3744 9.
7. Ito M, Deguchi T, Mizutani KS, Yasuda M, Yokoi S, Ito S, et al. Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in Central Japan. Antimicrob Agents Chemother 2005 49 : 137 43.
8. Lindberg R, Fredlund H, Nicholas R, Unemo M. Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponA. Antimicrob Agents Chemother 2007 51 : 2117 22.
9. Takahata S, Senju N, Osaki Y, Yoshida T, Ida T. Amino acid substitutions of mosaic penicillin-binding protein 2 associated with reduced susceptibility to cefixime in clinical isolates of Neisseria gonorrhoeae. Antimicrob Agents Chemother 2006 50 : 3638 45.
10. Tanaka M, Nakayama H, Huruya K, Konomi I, Irie S, Kanayama A, et al. Analysis of mutations within multiple genes associated with resistance in a clinical isolate of Neisseria gonorrhoeae with reduced ceftriaxone susceptibility that shows a multidrug-resistant phenotype. Int J Antimicrob Agents 2006 27 : 20 6.
11. Wang SA, Lee MV, O'Connor N, Iverson CJ, Ohye RG, Whiticar PM, et al. Multidrug-resistant Neisseria gonorrhoeae with decreased susceptibility to cefixime - Hawaii, 2001. Clin Infect Dis 2003 37 : 849 52.
12. Dowson CG, Jephcott AE, Gough KR, Spratt BG. Penicillin-binding protein 2 genes of non-beta-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae. Mol Microbiol 1989 3 : 35 41.
13. Ochiai S, Sekiguchi S, Hayashi A, Shimadzu M, Ishiko H, Matsushima-Nishiwaki R, et al. Decreased affinity of mosaic-structure recombinant penicillin-binding protein 2 for oral cephalosporins in Neisseria gonorrhoeae. J Antimicrob Chemother 2007 60 : 54 60.
14. Whiley DM, Limnios EA, Ray S, Sloots TP, Tapsall JW. Further questions regarding the role of mosaic penA sequences in conferring reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae. Antimicrob Agents Chemother 2007 51 : 802 3.
15. Whiley DM, Limnios EA, Ray S, Sloots TP, Tapsall JW. Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone. Antimicrob Agents Chemother 2007 51 : 3111 6.
16. Whiley D, Bates J, Limnios A, Nissen MD, Tapsall J, Sloots TP. Use of a novel screening PCR indicates presence of Neisseria gonorrhoeae isolates with a mosaic penA gene sequence in Australia. Pathology 2007 39 : 445 6.
17. Mavroidi A, Tzouvelekis LS, Kyriakis KP, Avgerinou H, Daniilidou M, Tzelepi E. Multidrug-resistant strains of Neisseria gonorrhoeae in Greece. Antimicrob Agents Chemother 2001 45 : 2651 4.
18. Thulin S, Olcén P, Fredlund H, Unemo M. A combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high throughput identification of reduced susceptibility to penicillins in Neisseria meningitidis. Antimicrob Agents Chemother 2007 52 : 753 6.