Cl--channel is essential for LDL-induced cell proliferation via the activation of Erk1/2 and PI3K/Akt and the upregulation of Egr-1 in human aortic smooth muscle cells

Molecules and Cells

Volumn 26, Issue 5, 2008, Pages 468-473

  Heo, Kyung Sun ^a,b   Ryoo, Sung Woo ^a   Kim, Lila ^a   Nam, Miyoung ^a   Baek, Seung Tae ^a   Lee, Hyemi ^a   Lee, Ah Reum ^a   Park, Song Kyu ^a   Park, Youngwoo ^a   Myung, Chang Seon ^b   Kim, Dong Uk ^a   Hoe, Kwang Lae ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

Cl channel; DIDS; Egr 1; Low density lipoprotein; PI3K

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 2 MORPHOLINO 8 PHENYLCHROMONE; 4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; 5 NITRO 2 (3 PHENYLPROPYLAMINO)BENZOIC ACID; CHLORIDE CHANNEL; CHLORIDE CHANNEL BLOCKING AGENT; CHLORIDE ION; EARLY GROWTH RESPONSE FACTOR 1; FUROSEMIDE; LOW DENSITY LIPOPROTEIN; MITOGEN ACTIVATED PROTEIN KINASE 1; PROTEIN KINASE B;

AORTA MEDIA; ARTICLE; CELL PROLIFERATION; CHANNEL GATING; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVATION; ENZYME INHIBITION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; SMOOTH MUSCLE FIBER; UPREGULATION; WESTERN BLOTTING;

1-PHOSPHATIDYLINOSITOL 3-KINASE; 4,4'-DIISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID; AORTA; CELL PROLIFERATION; CHLORIDE CHANNELS; CHOLESTEROL, LDL; DNA; EARLY GROWTH RESPONSE PROTEIN 1; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; HUMANS; INTRACELLULAR SPACE; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MYOCYTES, SMOOTH MUSCLE; PROTO-ONCOGENE PROTEINS C-AKT; UP-REGULATION;

EID: 58049099099     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Auge, N., Maupas-Schwalm, F., Elbaz, M., Thiers, J.C., Waysbort, A., Itohara, S., Krell, H.W., Salvayre, R., and Negre-Salvayre, A. (2004). Role for matrix metalloproteinase-2 in oxidized low-density lipoprotein-induced activation of the sphingomyelin/ceramide pathway and smooth muscle cell proliferation. Circulation 110, 571-578.
2. Bortner, C.D., and Cidlowski, J.A. (2004). The role of apoptotic volume decrease and ionic homeostasis in the activation and repression of apoptosis. Pflugers Arch. 448, 313-318.
3. Calabro, P., Samudio, I., Willerson, J.T., and Yeh, E.T. (2004). Resistin promotes smooth muscle cell proliferation through activation of extracellular signal-regulated kinase 1/2 and phosphatidylinositol 3-kinase pathways. Circulation 110, 3335-3340.
4. Castelli, W.P. (1984). Epidemiology of coronary heart disease: the Framingham study. Am. J. Med. 76, 4-12.
5. Duan, C., Bauchat, J.R., and Hsieh, T. (2000). Phosphatidylinositol 3-kinase is required for insulin-like growth factor-I-induced vascular smooth muscle cell proliferation and migration. Circ. Res. 86, 15-23.
6. Gervais, M., Dugourd, C., Muller, L., Ardidie, C., Canton, B., Loviconi, L., Corvol, P., Chneiweiss, H., and Monnot, C. (2006). Akt down-regulates ERK1/2 nuclear localization and angiotensin II-induced cell proliferation through PEA-15. Mol. Biol. Cell 17, 3940-3951.
7. Goncharova, E.A., Ammit, A.J., Irani, C., Carroll, R.G., Eszterhas, A.J., Panettieri, R.A., and Krymskaya, V.P. (2002). PI3K is required for proliferation and migration of human pulmonary vascular smooth muscle cells. Am. J. Physiol. Lung Cell Mol. Physiol. 283, L354-363.
8. Guan, Y.Y., Wang, G.L., and Zhou, J.G. (2006). The ClC-3 Cl(-) channel in cell volume regulation, proliferation and apoptosis in vascular smooth muscle cells. Trends Pharmacol. Sci. 27, 290-296.
9. Guillemot, L., Levy, A., Raymondjean, M., and Rothhut, B. (2001). Angiotensin II-induced transcriptional activation of the cyclin D1 gene is mediated by Egr-1 in CHO-AT(1A) cells. J. Biol. Chem. 276, 39394-39403.
10. Heo, K.S., Kim, D.U., Ryoo, S., Nam, M., Baek, S.T., Kim, L., Park, S.K., Myung, C.S., and Hoe, K.L. (2007). PPARγ activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide. Biochem. Biophys. Res. Commun. 359, 1017-1023.
11. II and PKCθ is essential for LDL-induced cell proliferation of human aortic smooth muscle cells via Gi-mediated Erk1/2 activation and Egr-1 upregulation. Biochem. Biophys. Res. Commun. 368, 126-131.
12. Kamimura, M., Bea, F., Akizawa, T., Katus, H.A., Kreuzer, J., and Viedt, C. (2004). Platelet-derived growth factor induces tissue factor expression in vascular smooth muscle cells via activation of Egr-1. Hypertension 44, 944-951.
13. Koncz, C., and Daugirdas, J.T. (1994). Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am. J. Physiol. 267, H2114-2123.
14. Li, R.C., Cindrova-Davies, T., Skepper, J.N., and Sellers, L.A. (2004). Prostacyclin induces apoptosis of vascular smooth muscle cells by a cAMP-mediated inhibition of extracellular signal-regulated kinase activity and can counteract the mitogenic activity of endothelin-1 or basic fibroblast growth factor. Circ. Res. 94, 759-767.
15. Locher, R., Brandes, R.P., Vetter, W., and Barton, M. (2002). Native LDL induces proliferation of human vascular smooth muscle cells via redox-mediated activation of ERK 1/2 mitogen-activated protein kinases. Hypertension 39, 645-650.
16. McCaffrey, T.A., Fu, C., Du, B., Eksinar, S., Kent, K.C., Bush, H., Jr., Kreiger, K., Rosengart, T., Cybulsky, M.I., Silverman, E.S., et al. (2000). High-level expression of Egr-1 and Egr-1-inducible genes in mouse and human atherosclerosis. J. Clin. Invest. 105, 653-662.
17. Nilius, B., and Droogmans, G. (2003). Amazing chloride channels: an overview. Acta Physiol. Scand. 177, 119-147.
18. Rocic, P., Govindarajan, G., Sabri, A., and Lucchesi, P.A. (2001). A role for PYK2 in regulation of ERK1/2 MAP kinases and PI 3-kinase by ANG II in vascular smooth muscle. Am. J. Physiol. Cell. Physiol. 280, C90-99.
19. Ross, R. (1999). Atherosclerosis - an inflammatory disease. N. Engl. J. Med. 340, 115-126.
20. Rouzaire-Dubois, B., Milandri, J.B., Bostel, S., and Dubois, J.M. (2000). Control of cell proliferation by cell volume alterations in rat C6 glioma cells. Pflugers Arch. 440, 881-888.
21. Ryoo, S.W., Kim, D.U., Won, M., Chung, K.S., Jang, Y.J., Oh, G.T., Park, S.K., Maeng, P.J., Yoo, H.S., and Hoe, K.L. (2004). Native LDL induces interleukin-8 expression via H2O2, p38 Kinase, and activator protein-1 in human aortic smooth muscle cells. Cardiovasc. Res. 62, 185-193.
22. Sachinidis, A., Mengden, T., Locher, R., Brunner, C., and Vetter, W. (1990). Novel cellular activities for low density lipoprotein in vascular smooth muscle cells. Hypertension 15, 704-711.
23. Sachinidis, A., Ko, Y., Wieczorek, A., Weisser, B., Locher, R., Vetter, W., and Vetter, H. (1993). Lipoproteins induce expression of the early growth response gene-1 in vascular smooth muscle cells from rat. Biochem. Biophys. Res. Commun. 192, 794-799.
24. Sachinidis, A., Schulte, K.B., Ko, Y., Seul, C., Meyer zu Brickwedde, M.K., Dusing, R., and Vetter, H. (1996). Oligodeoxynucleotides directed to early growth response gene-1 mRNA inhibit DNA synthesis in the smooth muscle cell. Eur. J. Pharmacol. 309, 95-105.
25. Santiago, F.S., Lowe, H.C., Kavurma, M.M., Chesterman, C.N., Baker, A., Atkins, D.G., and Khachigian, L.M. (1999). New DNA enzyme targeting Egr-1 mRNA inhibits vascular smooth muscle proliferation and regrowth after injury. Nat. Med. 5, 1264-1269.
26. Sarosi, G.A., Jr., Jaiswal, K., Herndon, E., Lopez-Guzman, C., Spechler, S.J., and Souza, R.F. (2005). Acid increases MAPK-mediated proliferation in Barrett's esophageal adenocarcinoma cells via intracellular acidification through a Cl-/HCO3- exchanger. Am. J. Physiol. Gastrointest. Liver Physiol. 289, G991-997.
27. Voets, T., Szucs, G., Droogmans, G., and Nilius, B. (1995). Blockers of volume-activated Cl- currents inhibit endothelial cell proliferation. Pflugers Arch. 431, 132-134.
28. Voets, T., Wei, L., De Smet, P., Van Driessche, W., Eggermont, J., Droogmans, G., and Nilius, B. (1997). Downregulation of volume-activated Cl- currents during muscle differentiation. Am. J. Physiol. 272, C667-674.
29. Watanabe, T., Pakala, R., Koba, S., Katagiri, T., and Benedict, C.R. (2001). Lysophosphatidylcholine and reactive oxygen species mediate the synergistic effect of mildly oxidized LDL with serotonin on vascular smooth muscle cell proliferation. Circulation 103, 1440-1445.
30. Wetzker, R., and Bohmer, F.D. (2003). Transactivation joins multiple tracks to the ERK/MAPK cascade. Nat. Rev. Mol. Cell. Biol. 4, 651-657.
31. Wondergem, R., Gong, W., Monen, S.H., Dooley, S.N., Gonce, J.L., Conner, T.D., Houser, M., Ecay, T.W., and Ferslew, K.E. (2001). Blocking swelling-activated chloride current inhibits mouse liver cell proliferation. J. Physiol. 532, 661-672.
32. Xiao, G.N., Guan, Y.Y., and He, H. (2002). Effects of Cl- channel blockers on endothelin-1-induced proliferation of rat vascular smooth muscle cells. Life Sci. 70, 2233-2241.
33. Zhou, J.G., Ren, J.L., Qiu, Q.Y., He, H., and Guan, Y.Y. (2005). Regulation of intracellular Cl- concentration through volume-regulated ClC-3 chloride channels in A10 vascular smooth muscle cells. J. Biol. Chem. 280, 7301-7308.