Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface

Acta Biomaterialia

Volumn 5, Issue 2, 2009, Pages 613-620

  Nakazawa, Kohji ^a   Izumi, Yumiko ^a   Mori, Ryuhei ^a  

^a UNIVERSITY OF KITAKYUSHU   (Japan)

Author keywords

FBS; Hydrophilic; Hydrophobic; PDMS; Rat hepatocytes

Indexed keywords

BODY FLUIDS; CELL ADHESION; GENE EXPRESSION; HYDROPHILICITY; MICROCHANNELS; MONOLAYERS; POLYDIMETHYLSILOXANE; RATS; SILICONES; WETTING;

CELLS ADHESION; COATED SURFACE; FBS; HEPATOCYTES; HYDROPHILIC SURFACES; HYDROPHILICS; HYDROPHOBICS; MORPHOLOGICAL BEHAVIOR; RAT HEPATOCYTES; SERUM-FREE MEDIA;

HYDROPHOBICITY;

ALBUMIN; AMMONIA; CADHERIN; CONNEXIN 32; DIMETICONE;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL COUNT; CELL FUNCTION; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; HYDROPHILICITY; HYDROPHOBICITY; LIVER CELL; LIVER CELL CULTURE; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SPHEROID CELL; SURFACE PROPERTY; WETTABILITY;

RATTUS;

EID: 57849141159     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2008.08.011     Document Type: Article

References (34)

1. Bissel M.D., Stamatoglou S.C., Nermut M.V., and Hughes R.C. Interactions of rat hepatocytes with type IV collagen, fibronectin and laminin. Distinct matrix-controlled modes of attachment and spreading. Eur J Cell Biol 40 (1986) 72-78
2. Koide N., Sakaguchi K., Koide Y., Asano K., Kawaguchi M., Matsushita H., et al. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186 (1990) 227-235
3. Tong J.Z., Lagausie P.D., Furlan V., Cresteil T., Bernard O., and Alvarez F. Long-term culture of adult rat hepatocyte spheroids. Exp Cell Res 200 (1992) 326-332
4. Tobe S., Takei Y., Kobayashi K., and Akaike T. Tissue reconstruction in primary cultured rat hepatocytes on a sialoglycoprotein model polymer. Artif Organs 16 (1992) 526-532
5. Powers M.J., Rodriguez R.E., and Griffith L.G. Cell-substratum adhesion strength as a determinant of hepatocyte aggregate morphology. Biotechnol Bioeng 53 (1997) 415-426
6. Ijima H., Matsushita T., Nakazawa K., Fujii Y., and Funatsu K. Hepatocyte spheroids in polyurethane foam: functional analysis and application for a hybrid artificial liver. Tissue Eng 4 (1998) 213-226
7. Fukuda J., and Nakazawa K. Orderly arrangement of hepatocyte spheroids on a microfabricated chip. Tissue Eng 11 (2005) 1254-1262
8. Sia S.K., and Whitesides G.M. Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies. Electrophoresis 24 (2003) 3563-3576
9. Makamba H., Kim J.H., Lim K., Park N., and Hahn J.H. Surface modification of poly(dimethylsiloxane) microchannels. Electrophoresis 24 (2003) 3607-3619
10. Singhvi R., Kumar A., Lopez G.P., Stephanopoulos G.N., Wang D.I.C., Whitesides G.M., et al. Engineering cell shape and function. Science 264 (1994) 696-698
11. Folch A., Jo B.H., Hurtado O., Beebe D.J., and Toner M. Microfabricated elastomeric stencils for micropatterning cell cultures. J Biomed Mater Res 52 (2000) 346-353
12. Krishna Y., Sheridan C.M., Kent D.L., Grierson I., and Williams R.L. Polydimethylsiloxane as a substrate for retinal pigment epithelial cell growth. J Biomed Mater Res 80A (2007) 669-678
13. Kidambi S., Udpa N., Schroeder S.A., Findlan R., Lee I., and Chan C. Cell adhesion on polyelectrolyte multilayer coated polydimethylsiloxane surfaces with varying topographies. Tissue Eng 13 (2007) 2105-2117
14. Prokop A., Prokop Z., Schaffer D., Kozlov E., Wikswo J., Cliffel D., et al. Nano liter bioreactor: long-term mammalian cell culture at nanofabricated scale. Biomed Microdevices 6 (2004) 325-339
15. Nakazawa K., Izumu Y., Fukuda J., and Yasuda T. Hepatocyte spheroid culture on a poly-dimethylsiloxane chip having microcavities. J Biomater Sci Polymer Edn 17 (2006) 859-873
16. Tzoneva R., Faucheux N., and Groth T. Wettability of substrata controls cell-substrate and cell-cell adhesions. Biochim Biophys Acta 1770 (2007) 1538-1547
17. Arima Y., and Iwata H. Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials 28 (2007) 3074-3082
18. Van Wachem P.B., Hogt A.H., Beugeling T., Feijen J., Bantjes A., Detmers J.P., et al. Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge. Biomaterials 8 (1987) 323-328
19. Groth T.H., Altankov G., and Klosz K. Adhesion of human peripheral blood lymphocytes is depending on surface wettability and protein preadsorption. Biomaterials 15 (1994) 423-428
20. Lee J.H., Lee J.W., Khang G., and Lee H.B. Interaction of cells on chargeable functional group gradient surfaces. Biomaterials 18 (1997) 351-358
21. Catapano G., Lorenzo M.C.D., Volpe C.D., Bartoro L., and Migliaresi C. Polymeric membranes for hybrid liver support devices: the effect of membrane surface wettability on hepatocyte viability and functions. J Biomater Sci Polymer Edn 11 (1996) 1017-1027
22. Krasteva N., Groth T.H., Fey-Lamprecht F., and Altankov G. The role of surface wettability on hepatocyte adhesive interactions and function. J Biomater Sci Polymer Edn 12 (2001) 613-627
23. De Bartolo L., Morelli S., Bader A., and Drioli E. Evaluation of cell behavior related to physico-chemical properties of polymeric membranes to be used in bioartificial organ. Biomaterials 23 (2002) 2485-2497
24. Grant M.H., Morgan C., Henderson C., Malsch G., Seifert B., Albrecht W., et al. The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices. J Biomed Mater Res A 73 (2005) 367-375
25. Seglen P.O. Preparation of isolated rat liver cells. Methods Cell Biol 13 (1976) 29-83
26. Ko I.K., Kato K., and Iwatq H. Parallel analysis of multiple surface markers expressed on rat neural stem cells using antibody microarrays. Biomaterials 26 (2005) 4882-4891
27. Williams G.M., Bermudez E., and Scaramuzzino D. Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium. In Vitro 13 (1977) 809-817
28. Tuschl G., and Mueller S.O. Effects of cell culture conditions on primary rat hepatocytes - cell morphology and differential gene expression. Toxicology 218 (2006) 205-215
29. Hou D.X., Arimura M., Fukuda M., Oka T., and Fujii M. Expression of cell adhesion molecule and albumin genes in primary culture of rat hepatocytes. Cell Biol Int 25 (2001) 239-244
30. Knudsen K.A., Frankowski C., Johnson K.R., and Wheelock M.J. A role for cadherins in cellular signaling and differentiation. J Cell Biochem Suppl 30 (1998) 168-176
31. Vleminckx K., and Kemler R. Cadherins and tissue formation: integrating adhesion and signaling. Bioessays 21 (1999) 211-220
32. Shimoyama Y., and Hirohashi S. Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma. Cancer Lett 57 (1991) 131-135
33. Fukuda J., Sakai Y., and Nakazawa K. Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing. Biomaterials 27 (2006) 1061-1070
34. Vinken M., Henkens T., De Rop E., Fraczek J., Vanhaecke T., and Rogiers V. Biology and pathobiology of gap junctional channels in hepatocytes. Hepatology 47 (2008) 1077-1088